Viral positive-sense RNA genomes evolve quickly because of the excessive mutation charges throughout replication and RNA recombination, which permitting the viruses to accumulate and modify genes for his or her adaptation. The dimensions of RNA genome is proscribed by a number of elements, together with low constancy of RNA polymerases and packaging constraints. Nevertheless, the 12-kb measurement restrict is exceeded within the two teams of eukaryotic (+)RNA viruses – animal nidoviruses and plant closteroviruses. These virus teams have a number of traits in frequent.
Their genomes include 5′-proximal genes which might be expressed by way of ribosomal frameshifting and encode one or two papain-like protease domains, membrane-binding area(s), methyltransferase, RNA helicase, and RNA polymerase. As well as, some nidoviruses (i.e., coronaviruses) include replication-associated domains, equivalent to proofreading exonuclease, putative primase, nucleotidyltransferase, and endonuclease. In each nidoviruses and closteroviruses, the three’-terminal a part of the genome incorporates genes for structural and accent proteins expressed by way of a nested set of coterminal subgenomic RNAs.
Coronaviruses and closteroviruses have developed to kind flexuous helically symmetrical nucleocapsids as a imply to resolve packaging constraints. Since phylogenetic reconstructions of the RNA polymerase domains point out solely a marginal relationship between the nidoviruses and closteroviruses, their comparable properties seemingly have developed convergently, together with the rise within the genome measurement.
Construction and meeting of double-stranded RNA mycoviruses
Mycoviruses are a various group that features ssRNA, dsRNA, and ssDNA viruses, with or and not using a protein capsid, in addition to with a posh envelope. Most mycoviruses are transmitted by cytoplasmic interchange and are thought to lack an extracellular section of their an infection cycle. Structural evaluation has centered on dsRNA mycoviruses, which normally bundle their genome in a 120-subunit T=1 icosahedral capsid, with a capsid protein (CP) dimer because the uneven unit.
The atomic construction is out there for 4 dsRNA mycovirus from completely different households: Saccharomyces cerevisiae virus L-A (ScV-L-A), Penicillium chrysogenum virus (PcV), Penicillium stoloniferum virus F (PsV-F), and Rosellinia necatrix quadrivirus 1 (RnQV1). Their capsids present structural variations of the identical framework, with uneven or symmetric CP dimers respectively for ScV-L-A and PsV-F, dimers of comparable domains of a single CP for PcV, or of two completely different proteins for RnQV1. The CP dimer is the constructing block, and meeting proceeds via dimers of dimers or pentamers of dimers, during which the genome is packed as ssRNA by interplay with CP and/or viral polymerase.
These capsids stay structurally undisturbed all through the viral cycle. The T=1 capsid participates in RNA synthesis, organizing the viral polymerase (1-2 copies) and a single loosely packaged genome phase. It additionally acts as a molecular sieve, to permit the passage of viral transcripts and nucleotides, however to forestall triggering of host protection mechanisms. Because of the shut mycovirus-host relationship, CP developed to allocate peptide insertions with enzyme exercise, as mirrored in a tough outer capsid floor.
Dynamically probing ATP-dependent RHA-assisted RNA construction conversion utilizing single molecule fluorescence resonance power switch
RNA helicase A (RHA) as a member of DExH-box subgroup of helicase superfamily II, participates in numerous organic processes concerned in RNA metabolism in organisms, and these RNA-mediated organic processes depend on RNA construction conversion. Nevertheless, how RHA regulate the RNA construction conversion was nonetheless unknown. To be able to unveil the mechanism of RNA construction conversion mediated by RHA, single molecule fluorescence resonance power switch (smFRET) was adopted to in our assay, and substrates RNA had been from inner ribosome entry web site (IRES) of foot-and-mouth illness virus (FMDV) genome. We first discovered that the RNA construction conversion by RHA towards thermodynamic equilibrium in vitro, and the method of dsRNA YZ transformed to dsRNA XY via a tripartite intermediate state.
As well as, the speed of the RNA construction conversion and the distribution of dsRNA YZ and XY had been affected by ATP concentrations. Our research supplies real-time perception into ATP-dependent RHA-assisted RNA construction conversion on the single molecule stage, the mechanism displayed by RHA could assist in perceive how RHA contributes to many organic features, and the essential mechanistic options illustrated in our work additionally underlay extra complicated protein-assisted RNA construction conversions. This text is protected by copyright. All rights reserved.
Modeling and Predicting RNA Three-Dimensional Buildings
Modeling the three-dimensional construction of RNAs is a milestone towards higher understanding and prediction of nucleic acids molecular features. Physics-based approaches and molecular dynamics simulations are usually not tractable on giant molecules with all-atom fashions. To handle this challenge, coarse-grained fashions of RNA three-dimensional buildings have been developed. On this chapter, we describe a graphical modeling primarily based on the Leontis-Westhof prolonged base pair classification. This illustration of RNA buildings permits us to determine extremely conserved structural motifs with complicated nucleotide interactions in construction databases.
We present how one can make the most of this information to shortly predict three-dimensional buildings of enormous RNA molecules and current the RNA-MoIP net server (http://rnamoip.cs.mcgill.ca) that streamlines the computational and visualization processes. Lastly, we present current advances within the prediction of native 3D motifs from sequence information with the BayesPairing software program and focus on its affect towards full 3D construction prediction.
Profiling of RNA Construction at Single-Nucleotide Decision Utilizing nextPARS
RNA molecules play essential roles in nearly each mobile course of, and their features are mediated by their sequence and construction. Figuring out the secondary construction of RNAs is central to understanding RNA perform and evolution. RNA construction probing methods coupled to high-throughput sequencing enable figuring out structural options of RNA molecules at transcriptome-wide scales.
 Mouse C57 Mammary Gland, E14 cDNA |
|
MD-414-14-C57 |
Zyagen |
30 reactions |
EUR 280 |
 Mouse CD1 Mammary Gland, E15 cDNA |
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MD-414-15 |
Zyagen |
30 reactions |
EUR 243 |
 Mouse C57 Mammary Gland, E15 cDNA |
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MD-414-15-C57 |
Zyagen |
30 reactions |
EUR 280 |
 Mouse CD1 Mammary Gland, E16 cDNA |
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MD-414-16 |
Zyagen |
30 reactions |
EUR 243 |
 Mouse C57 Mammary Gland, E16 cDNA |
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MD-414-16-C57 |
Zyagen |
30 reactions |
EUR 280 |
 Mouse CD1 Mammary Gland, E17 cDNA |
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MD-414-17 |
Zyagen |
30 reactions |
EUR 243 |
 Mouse C57 Mammary Gland, E17 cDNA |
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MD-414-17-C57 |
Zyagen |
30 reactions |
EUR 280 |
 Mouse CD1 Mammary Gland, E18 cDNA |
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MD-414-18 |
Zyagen |
30 reactions |
EUR 243 |
 Mouse C57 Mammary Gland, E18 cDNA |
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MD-414-18-C57 |
Zyagen |
30 reactions |
EUR 280 |
 Mouse CD1 Mammary Gland Western Blot |
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MW-414 |
Zyagen |
1 Blot |
EUR 668 |
 Mouse C57 Mammary Gland Western Blot |
|
MW-414-C57 |
Zyagen |
1 Blot |
EUR 789 |
 Mouse CD1 Mammary Gland, E11 Total RNA |
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MR-414-11 |
Zyagen |
0.05mg |
EUR 160 |
 Mouse C57 Mammary Gland, E11 Total RNA |
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MR-414-11-C57 |
Zyagen |
0.025mg |
EUR 180 |
 Mouse CD1 Mammary Gland, E12 Total RNA |
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MR-414-12 |
Zyagen |
0.05mg |
EUR 160 |
 Mouse C57 Mammary Gland, E12 Total RNA |
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MR-414-12-C57 |
Zyagen |
0.025mg |
EUR 180 |
 Mouse CD1 Mammary Gland, E13 Total RNA |
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MR-414-13 |
Zyagen |
0.05mg |
EUR 160 |
 Mouse C57 Mammary Gland, E13 Total RNA |
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MR-414-13-C57 |
Zyagen |
0.025mg |
EUR 180 |
 Mouse CD1 Mammary Gland, E14 Total RNA |
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MR-414-14 |
Zyagen |
0.05mg |
EUR 160 |
 Mouse C57 Mammary Gland, E14 Total RNA |
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MR-414-14-C57 |
Zyagen |
0.05mg |
EUR 180 |
 Mouse CD1 Mammary Gland, E15 Total RNA |
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MR-414-15 |
Zyagen |
0.05mg |
EUR 160 |
 Mouse C57 Mammary Gland, E15 Total RNA |
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MR-414-15-C57 |
Zyagen |
0.05mg |
EUR 180 |
 Mouse CD1 Mammary Gland, E16 Total RNA |
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MR-414-16 |
Zyagen |
0.05mg |
EUR 160 |
 Mouse C57 Mammary Gland, E16 Total RNA |
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MR-414-16-C57 |
Zyagen |
0.05mg |
EUR 180 |
 Mouse CD1 Mammary Gland, E17 Total RNA |
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MR-414-17 |
Zyagen |
0.05mg |
EUR 160 |
 Mouse C57 Mammary Gland, E17 Total RNA |
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MR-414-17-C57 |
Zyagen |
0.05mg |
EUR 180 |
 Mouse CD1 Mammary Gland, E18 Total RNA |
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MR-414-18 |
Zyagen |
0.05mg |
EUR 160 |
 Mouse C57 Mammary Gland, E18 Total RNA |
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MR-414-18-C57 |
Zyagen |
0.05mg |
EUR 180 |
 Mouse Mammary Gland Developmental Western Blot |
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MW-414-D |
Zyagen |
1 Blot |
EUR 910 |
 Mouse CD1 Mammary Gland, non pregnant cDNA |
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MD-414 |
Zyagen |
30 reactions |
EUR 243 |
Mouse CD1 Mammary Gland, non pregnant cDNA |
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MD-414-1 |
Zyagen |
30 reactions |
EUR 243 |
 Mouse BLC Mammary Gland, non pregnant cDNA |
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MD-414-BLC |
Zyagen |
30 reactions |
EUR 280 |
 Mouse C57 Mammary Gland, non pregnant cDNA |
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MD-414-C57 |
Zyagen |
30 reactions |
EUR 280 |
Mouse C57 Mammary Gland, non pregnant cDNA |
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MD-414-C57-1 |
Zyagen |
30 reactions |
EUR 280 |
 Mouse CD1 Mammary Gland, E11 Total Protein |
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MT-414-11 |
Zyagen |
0.5mg |
EUR 153 |
 Mouse C57 Mammary Gland, E11 Total Protein |
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MT-414-11-C57 |
Zyagen |
0.5mg |
EUR 180 |
 Mouse CD1 Mammary Gland, E12 Total Protein |
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MT-414-12 |
Zyagen |
0.5mg |
EUR 153 |
 Mouse C57 Mammary Gland, E12 Total Protein |
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MT-414-12-C57 |
Zyagen |
0.5mg |
EUR 180 |
 Mouse CD1 Mammary Gland, E13 Total Protein |
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MT-414-13 |
Zyagen |
0.5mg |
EUR 153 |
 Mouse C57 Mammary Gland, E13 Total Protein |
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MT-414-13-C57 |
Zyagen |
0.5mg |
EUR 180 |
 Mouse CD1 Mammary Gland, E14 Total Protein |
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MT-414-14 |
Zyagen |
0.5mg |
EUR 153 |
 Mouse C57 Mammary Gland, E14 Total Protein |
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MT-414-14-C57 |
Zyagen |
0.5mg |
EUR 180 |
 Mouse CD1 Mammary Gland, E15 Total Protein |
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MT-414-15 |
Zyagen |
0.5mg |
EUR 153 |
 Mouse C57 Mammary Gland, E15 Total Protein |
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MT-414-15-C57 |
Zyagen |
0.5mg |
EUR 180 |
 Mouse CD1 Mammary Gland, E16 Total Protein |
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MT-414-16 |
Zyagen |
0.5mg |
EUR 153 |
 Mouse C57 Mammary Gland, E16 Total Protein |
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MT-414-16-C57 |
Zyagen |
0.5mg |
EUR 180 |
 Mouse CD1 Mammary Gland, E17 Total Protein |
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MT-414-17 |
Zyagen |
0.5mg |
EUR 153 |
 Mouse C57 Mammary Gland, E17 Total Protein |
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MT-414-17-C57 |
Zyagen |
0.5mg |
EUR 180 |
 Mouse CD1 Mammary Gland, E18 Total Protein |
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MT-414-18 |
Zyagen |
0.5mg |
EUR 153 |
 Mouse C57 Mammary Gland, E18 Total Protein |
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MT-414-18-C57 |
Zyagen |
0.5mg |
EUR 180 |
 Mouse CD1 Mammary Gland Frozen Sections, E11 |
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MF-414-11 |
Zyagen |
10 slides |
EUR 253 |
 Mouse C57 Mammary Gland Frozen Sections, E11 |
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MF-414-11-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Frozen Sections, E12 |
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MF-414-12 |
Zyagen |
10 slides |
EUR 253 |
 Mouse C57 Mammary Gland Frozen Sections, E12 |
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MF-414-12-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Frozen Sections, E13 |
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MF-414-13 |
Zyagen |
10 slides |
EUR 253 |
 Mouse C57 Mammary Gland Frozen Sections, E13 |
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MF-414-13-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Frozen Sections, E14 |
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MF-414-14 |
Zyagen |
10 slides |
EUR 253 |
 Mouse C57 Mammary Gland Frozen Sections, E14 |
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MF-414-14-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Frozen Sections, E15 |
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MF-414-15 |
Zyagen |
10 slides |
EUR 253 |
 Mouse C57 Mammary Gland Frozen Sections, E15 |
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MF-414-15-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Frozen Sections, E16 |
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MF-414-16 |
Zyagen |
10 slides |
EUR 253 |
 Mouse C57 Mammary Gland Frozen Sections, E16 |
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MF-414-16-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Frozen Sections, E17 |
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MF-414-17 |
Zyagen |
10 slides |
EUR 253 |
 Mouse C57 Mammary Gland Frozen Sections, E17 |
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MF-414-17-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Frozen Sections, E18 |
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MF-414-18 |
Zyagen |
10 slides |
EUR 253 |
 Mouse C57 Mammary Gland Frozen Sections, E18 |
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MF-414-18-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Paraffin Sections, E11 |
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MP-414-11 |
Zyagen |
10 slides |
EUR 266 |
 Mouse C57 Mammary Gland Paraffin Sections, E11 |
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MP-414-11-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Paraffin Sections, E12 |
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MP-414-12 |
Zyagen |
10 slides |
EUR 266 |
 Mouse C57 Mammary Gland Paraffin Sections, E12 |
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MP-414-12-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Paraffin Sections, E13 |
|
MP-414-13 |
Zyagen |
10 slides |
EUR 266 |
 Mouse C57 Mammary Gland Paraffin Sections, E13 |
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MP-414-13-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Paraffin Sections, E14 |
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MP-414-14 |
Zyagen |
10 slides |
EUR 266 |
 Mouse C57 Mammary Gland Paraffin Sections, E14 |
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MP-414-14-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Paraffin Sections, E15 |
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MP-414-15 |
Zyagen |
10 slides |
EUR 266 |
 Mouse C57 Mammary Gland Paraffin Sections, E15 |
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MP-414-15-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Paraffin Sections, E16 |
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MP-414-16 |
Zyagen |
10 slides |
EUR 266 |
 Mouse C57 Mammary Gland Paraffin Sections, E16 |
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MP-414-16-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Paraffin Sections, E17 |
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MP-414-17 |
Zyagen |
10 slides |
EUR 266 |
 Mouse C57 Mammary Gland Paraffin Sections, E17 |
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MP-414-17-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Paraffin Sections, E18 |
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MP-414-18 |
Zyagen |
10 slides |
EUR 266 |
 Mouse C57 Mammary Gland Paraffin Sections, E18 |
|
MP-414-18-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Premade Northern Blot |
|
MN-414 |
Zyagen |
1 Blot |
EUR 668 |
 Mouse C57 Mammary Gland Premade Northern Blot |
|
MN-414-C57 |
Zyagen |
1 Blot |
EUR 903 |
 Mouse CD1 Mammary Gland Frozen Sections, Virgin |
|
MF-414-V |
Zyagen |
10 slides |
EUR 253 |
 Mouse C57 Mammary Gland Frozen Sections, Virgin |
|
MF-414-V-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Paraffin Sections, Virgin |
|
MP-414-V |
Zyagen |
10 slides |
EUR 266 |
 Mouse C57 Mammary Gland Paraffin Sections, Virgin |
|
MP-414-V-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland, non pregnant Total RNA |
|
MR-414 |
Zyagen |
0.05mg |
EUR 160 |
 Mouse C57 Mammary Gland, non pregnant Total RNA |
|
MR-414-C57 |
Zyagen |
0.025mg |
EUR 180 |
 Rabbit Mammary Gland cDNA |
|
TD-414 |
Zyagen |
30 reactions |
EUR 243 |
 Mouse Balbc Mammary Gland, non pregnant Total RNA |
|
MR-414-BLC |
Zyagen |
0.05mg |
EUR 180 |
 Mouse CD1 Mammary Gland, non pregnant Total Protein |
|
MT-414 |
Zyagen |
0.5mg |
EUR 153 |
 Mouse BLC Mammary Gland, non pregnant Total Protein |
|
MT-414-BLC |
Zyagen |
0.5mg |
EUR 180 |
 Mouse C57 Mammary Gland, non pregnant Total Protein |
|
MT-414-C57 |
Zyagen |
0.5mg |
EUR 180 |
 Mouse CD1 Mammary Gland, Day 1 of Lactation cDNA |
|
MD-414-L1 |
Zyagen |
30 reactions |
EUR 243 |
 Mouse C57 Mammary Gland, Day 1 of Lactation cDNA |
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MD-414-L1-C57 |
Zyagen |
30 reactions |
EUR 280 |
 Mouse CD1 Mammary Gland, Day 3 of Lactation cDNA |
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MD-414-L3 |
Zyagen |
30 reactions |
EUR 243 |
 Mouse C57 Mammary Gland, Day 3 of Lactation cDNA |
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MD-414-L3-C57 |
Zyagen |
30 reactions |
EUR 280 |
 Mouse CD1 Mammary Gland, Day 7 of Lactation cDNA |
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MD-414-L7 |
Zyagen |
30 reactions |
EUR 243 |
 Mouse C57 Mammary Gland, Day 7 of Lactation cDNA |
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MD-414-L7-C57 |
Zyagen |
30 reactions |
EUR 280 |
 Mouse CD1 Mammary Gland Frozen Sections, Lactation D1 |
|
MF-414-L1 |
Zyagen |
10 slides |
EUR 253 |
 Mouse C57 Mammary Gland Frozen Sections, Lactation D1 |
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MF-414-L1-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Frozen Sections, Lactation D3 |
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MF-414-L3 |
Zyagen |
10 slides |
EUR 253 |
 Mouse C57 Mammary Gland Frozen Sections, Lactation D3 |
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MF-414-L3-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Frozen Sections, Lactation D7 |
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MF-414-L7 |
Zyagen |
10 slides |
EUR 253 |
 Mouse C57 Mammary Gland Frozen Sections, Lactation D7 |
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MF-414-L7-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland, Day 1 of Involution cDNA |
|
MD-414-N1 |
Zyagen |
30 reactions |
EUR 243 |
 Mouse C57 Mammary Gland, Day 1 of Involution cDNA |
|
MD-414-N1-C57 |
Zyagen |
30 reactions |
EUR 280 |
 Mouse CD1 Mammary Gland, Day 7 of Involution cDNA |
|
MD-414-N7 |
Zyagen |
30 reactions |
EUR 243 |
 Mouse C57 Mammary Gland, Day 7 of Involution cDNA |
|
MD-414-N7-C57 |
Zyagen |
30 reactions |
EUR 280 |
 Mouse CD1 Mammary Gland Frozen Sections, Involution D1 |
|
MF-414-N1 |
Zyagen |
10 slides |
EUR 253 |
 Mouse C57 Mammary Gland Frozen Sections, Involution D1 |
|
MF-414-N1-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Frozen Sections, Involution D7 |
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MF-414-N7 |
Zyagen |
10 slides |
EUR 253 |
 Mouse C57 Mammary Gland Frozen Sections, Involution D7 |
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MF-414-N7-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Frozen Sections, Non-Pregnant |
|
MF-414 |
Zyagen |
10 slides |
EUR 266 |
 Mouse BLC Mammary Gland Frozen Sections, Non-Pregnant |
|
MF-414-BLC |
Zyagen |
10 slides |
EUR 291 |
 Mouse C57 Mammary Gland Frozen Sections, Non-Pregnant |
|
MF-414-C57 |
Zyagen |
10 slides |
EUR 277 |
 Mouse CD1 Mammary Gland Paraffin Sections, Lactation D1 |
|
MP-414-L1 |
Zyagen |
10 slides |
EUR 266 |
 Mouse C57 Mammary Gland Paraffin Sections, Lactation D1 |
|
MP-414-L1-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Paraffin Sections, Lactation D3 |
|
MP-414-L3 |
Zyagen |
10 slides |
EUR 266 |
 Mouse C57 Mammary Gland Paraffin Sections, Lactation D3 |
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MP-414-L3-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Paraffin Sections, Lactation D7 |
|
MP-414-L7 |
Zyagen |
10 slides |
EUR 266 |
 Mouse C57 Mammary Gland Paraffin Sections, Lactation D7 |
|
MP-414-L7-C57 |
Zyagen |
10 slides |
EUR 291 |
 Rat Mammary Gland, E12 cDNA |
|
RD-414-12 |
Zyagen |
30 reactions |
EUR 243 |
 Rat Mammary Gland, E13 cDNA |
|
RD-414-13 |
Zyagen |
30 reactions |
EUR 243 |
 Rat Mammary Gland, E14 cDNA |
|
RD-414-14 |
Zyagen |
30 reactions |
EUR 243 |
 Rat Mammary Gland, E15 cDNA |
|
RD-414-15 |
Zyagen |
30 reactions |
EUR 243 |
 Rat Mammary Gland, E16 cDNA |
|
RD-414-16 |
Zyagen |
30 reactions |
EUR 243 |
 Rat Mammary Gland, E17 cDNA |
|
RD-414-17 |
Zyagen |
30 reactions |
EUR 243 |
 Rat Mammary Gland, E18 cDNA |
|
RD-414-18 |
Zyagen |
30 reactions |
EUR 243 |
 Rat Mammary Gland, E19 cDNA |
|
RD-414-19 |
Zyagen |
30 reactions |
EUR 243 |
 Rat Mammary Gland, E20 cDNA |
|
RD-414-20 |
Zyagen |
30 reactions |
EUR 243 |
 Mouse CD1 Mammary Gland Paraffin Sections, Involution D1 |
|
MP-414-N1 |
Zyagen |
10 slides |
EUR 266 |
 Mouse C57 Mammary Gland Paraffin Sections, Involution D1 |
|
MP-414-N1-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Paraffin Sections, Involution D7 |
|
MP-414-N7 |
Zyagen |
10 slides |
EUR 266 |
 Mouse C57 Mammary Gland Paraffin Sections, Involution D7 |
|
MP-414-N7-C57 |
Zyagen |
10 slides |
EUR 291 |
 Mouse CD1 Mammary Gland Paraffin Sections, Non-Pregnant |
|
MP-414 |
Zyagen |
10 slides |
EUR 266 |
 Mouse BLC Mammary Gland Paraffin Sections, Non-Pregnant |
|
MP-414-BLC |
Zyagen |
10 slides |
EUR 291 |
 Mouse C57 Mammary Gland Paraffin Sections, Non-Pregnant |
|
MP-414-C57 |
Zyagen |
10 slides |
EUR 291 |
 Rat Mammary Gland Western Blot |
|
RW-414 |
Zyagen |
1 Blot |
EUR 668 |
 Rabbit Mammary Gland Total RNA |
|
TR-414 |
Zyagen |
0.05mg |
EUR 160 |
 Mouse CD1 Mammary Gland, Day 1 of Lactation Total RNA |
|
MR-414-L1 |
Zyagen |
0.05mg |
EUR 160 |
 Mouse C57 Mammary Gland, Day 1 of Lactation Total RNA |
|
MR-414-L1-C57 |
Zyagen |
0.05mg |
EUR 180 |
 Mouse CD1 Mammary Gland, Day 3 of Lactation Total RNA |
|
MR-414-L3 |
Zyagen |
0.05mg |
EUR 160 |
 Mouse C57 Mammary Gland, Day 3 of Lactation Total RNA |
|
MR-414-L3-C57 |
Zyagen |
0.05mg |
EUR 180 |
 Mouse CD1 Mammary Gland, Day 7 of Lactation Total RNA |
|
MR-414-L7 |
Zyagen |
0.05mg |
EUR 160 |
 Mouse C57 Mammary Gland, Day 7 of Lactation Total RNA |
|
MR-414-L7-C57 |
Zyagen |
0.05mg |
EUR 180 |
 Mouse CD1 Mammary Gland, Day 1 of involution Total RNA |
|
MR-414-N1 |
Zyagen |
0.05mg |
EUR 160 |
 Mouse C57 Mammary Gland, Day 1 of involution Total RNA |
|
MR-414-N1-C57 |
Zyagen |
0.05mg |
EUR 180 |
 Mouse CD1 Mammary Gland, Day 7 of involution Total RNA |
|
MR-414-N7 |
Zyagen |
0.05mg |
EUR 160 |
 Mouse C57 Mammary Gland, Day 7 of involution Total RNA |
|
MR-414-N7-C57 |
Zyagen |
0.05mg |
EUR 180 |
 Mouse C57 Mammary Gland, non pregnant cDNA-Random Primer |
|
MD-414-C57-RH |
Zyagen |
30 reactions |
EUR 280 |
 Mouse CD1 Mammary Gland, non pregnant cDNA-Random Primer |
|
MD-414-HR |
Zyagen |
30 reactions |
EUR 243 |
 Mouse CD1 Mammary Gland, Day 1 of Lactation Total Protein |
|
MT-414-L1 |
Zyagen |
0.5mg |
EUR 153 |
 Mouse C57 Mammary Gland, Day 1 of Lactation Total Protein |
|
MT-414-L1-C57 |
Zyagen |
0.5mg |
EUR 180 |
 Mouse CD1 Mammary Gland, Day 3 of Lactation Total Protein |
|
MT-414-L3 |
Zyagen |
0.5mg |
EUR 153 |
 Mouse C57 Mammary Gland, Day 3 of Lactation Total Protein |
|
MT-414-L3-C57 |
Zyagen |
0.5mg |
EUR 180 |
 Mouse CD1 Mammary Gland, Day 7 of Lactation Total Protein |
|
MT-414-L7 |
Zyagen |
0.5mg |
EUR 153 |
 Mouse C57 Mammary Gland, Day 7 of Lactation Total Protein |
|
MT-414-L7-C57 |
Zyagen |
0.5mg |
EUR 180 |
 Rat Mammary Gland, E12 Total RNA |
|
RR-414-12 |
Zyagen |
0.05mg |
EUR 160 |
 Rat Mammary Gland, E13 Total RNA |
|
RR-414-13 |
Zyagen |
0.05mg |
EUR 160 |
 Rat Mammary Gland, E14 Total RNA |
|
RR-414-14 |
Zyagen |
0.05mg |
EUR 160 |
 Rat Mammary Gland, E15 Total RNA |
|
RR-414-15 |
Zyagen |
0.05mg |
EUR 160 |
 Rat Mammary Gland, E16 Total RNA |
|
RR-414-16 |
Zyagen |
0.05mg |
EUR 160 |
 Rat Mammary Gland, E17 Total RNA |
|
RR-414-17 |
Zyagen |
0.05mg |
EUR 160 |
 Rat Mammary Gland, E18 Total RNA |
|
RR-414-18 |
Zyagen |
0.05mg |
EUR 160 |
 Rat Mammary Gland, E19 Total RNA |
|
RR-414-19 |
Zyagen |
0.05mg |
EUR 160 |
 Rat Mammary Gland, E20 Total RNA |
|
RR-414-20 |
Zyagen |
0.05mg |
EUR 160 |
 Mouse CD1 Mammary Gland, Day 1 of Involution Total Protein |
|
MT-414-N1 |
Zyagen |
0.5mg |
EUR 153 |
 Mouse C57 Mammary Gland, Day 1 of Involution Total Protein |
|
MT-414-N1-C57 |
Zyagen |
0.5mg |
EUR 180 |
 Mouse CD1 Mammary Gland, Day 7 of Involution Total Protein |
|
MT-414-N7 |
Zyagen |
0.5mg |
EUR 153 |
 Mouse C57 Mammary Gland, Day 7 of Involution Total Protein |
|
MT-414-N7-C57 |
Zyagen |
0.5mg |
EUR 180 |
 Mouse CD1 Mammary Gland cDNA Panel, set of any 5 Tissues |
|
MD-414-005 |
Zyagen |
5x10 reactions |
EUR 867 |
 Mouse C57 Mammary Gland cDNA Panel, set of any 5 Tissues |
|
MD-414-005-C57 |
Zyagen |
5x10 reactions |
EUR 1100 |
 Mouse CD1 Mammary Gland cDNA Panel, set of any 10 Tissues |
|
MD-414-010 |
Zyagen |
10x10 reactions |
EUR 1158 |
 Mouse C57 Mammary Gland cDNA Panel, set of any 10 Tissues |
|
MD-414-010-C57 |
Zyagen |
10x10 reactions |
EUR 1434 |
 Mouse CD1 Mammary Gland cDNA Panel, set of any 14 Tissues |
|
MD-414-014 |
Zyagen |
14x10reactions |
EUR 1449 |
 Mouse C57 Mammary Gland cDNA Panel, set of any 14 Tissues |
|
MD-414-014-C57 |
Zyagen |
14x10reactions |
EUR 1731 |
 Mouse CD1 mammary gland Frozen Sections Panel, any 15 stages |
|
MF-414-015 |
Zyagen |
15X4slides |
EUR 987 |
 Mouse C57 mammary gland Frozen Sections Panel, any 15 stages |
|
MF-414-015-C57 |
Zyagen |
15X4slides |
EUR 1109 |
 Mouse CD1 mammary gland Frozen Sections Panel, any 8 tissues |
|
MF-414-008 |
Zyagen |
8X4 slides |
EUR 710 |
 Mouse C57 mammary gland Frozen Sections Panel, any 8 tissues |
|
MF-414-008-C57 |
Zyagen |
8X4 slides |
EUR 818 |
 Mouse CD1 Mammary Gland Paraffin Sections Panel, any 8 Stages |
|
MP-414-008 |
Zyagen |
8X4 slides |
EUR 744 |
 Mouse C57 mammary gland Paraffin Sections Panel, any 8 stages |
|
MP-414-008-C57 |
Zyagen |
8X4 slides |
EUR 818 |
 Mouse CD1 Mammary Gland Paraffin Sections Panel, any 15 Stages |
|
MP-414-015 |
Zyagen |
15X4slides |
EUR 1035 |
 Mouse C57 mammary gland Paraffin Sections Panel, any 15 stages |
|
MP-414-015-C57 |
Zyagen |
15X4slides |
EUR 1109 |
 Guinea Pig Mammary Gland-E20 cDNA |
|
GD-414-20 |
Zyagen |
30 reactions |
EUR 280 |
 Guinea Pig Mammary Gland-E25 cDNA |
|
GD-414-25 |
Zyagen |
30 reactions |
EUR 280 |
 Guinea Pig Mammary Gland-E35 cDNA |
|
GD-414-35 |
Zyagen |
30 reactions |
EUR 280 |
 Guinea Pig Mammary Gland-E40 cDNA |
|
GD-414-40 |
Zyagen |
30 reactions |
EUR 280 |
 Rat Mammary Gland, E12 Total Protein |
|
RT-414-12 |
Zyagen |
0.5mg |
EUR 153 |
 Rat Mammary Gland, E13 Total Protein |
|
RT-414-13 |
Zyagen |
0.5mg |
EUR 153 |
 Rat Mammary Gland, E14 Total Protein |
|
RT-414-14 |
Zyagen |
0.5mg |
EUR 153 |
 Rat Mammary Gland, E15 Total Protein |
|
RT-414-15 |
Zyagen |
0.5mg |
EUR 153 |
 Rat Mammary Gland, E16 Total Protein |
|
RT-414-16 |
Zyagen |
0.5mg |
EUR 153 |
 Rat Mammary Gland, E17 Total Protein |
|
RT-414-17 |
Zyagen |
0.5mg |
EUR 153 |
Our group lately developed a novel Illumina-based implementation of in vitro parallel probing of RNA buildings referred to as nextPARS.Right here, we describe a protocol for the computation of the nextPARS scores and their use to acquire the structural profile (single- or double-stranded state) of an RNA sequence at single-nucleotide decision.
