Structure and Expression of Large (+)RNA Genomes of Viruses of Higher Eukaryotes

Structure and Expression of Large (+)RNA Genomes of Viruses of Higher Eukaryotes
Viral positive-sense RNA genomes evolve quickly because of the excessive mutation charges throughout replication and RNA recombination, which permitting the viruses to accumulate and modify genes for his or her adaptation. The dimensions of RNA genome is proscribed by a number of elements, together with low constancy of RNA polymerases and packaging constraints. Nevertheless, the 12-kb measurement restrict is exceeded within the two teams of eukaryotic (+)RNA viruses – animal nidoviruses and plant closteroviruses. These virus teams have a number of traits in frequent.
Their genomes include 5′-proximal genes which might be expressed by way of ribosomal frameshifting and encode one or two papain-like protease domains, membrane-binding area(s), methyltransferase, RNA helicase, and RNA polymerase. As well as, some nidoviruses (i.e., coronaviruses) include replication-associated domains, equivalent to proofreading exonuclease, putative primase, nucleotidyltransferase, and endonuclease. In each nidoviruses and closteroviruses, the three’-terminal a part of the genome incorporates genes for structural and accent proteins expressed by way of a nested set of coterminal subgenomic RNAs.
Coronaviruses and closteroviruses have developed to kind flexuous helically symmetrical nucleocapsids as a imply to resolve packaging constraints. Since phylogenetic reconstructions of the RNA polymerase domains point out solely a marginal relationship between the nidoviruses and closteroviruses, their comparable properties seemingly have developed convergently, together with the rise within the genome measurement.

Construction and meeting of double-stranded RNA mycoviruses

Mycoviruses are a various group that features ssRNA, dsRNA, and ssDNA viruses, with or and not using a protein capsid, in addition to with a posh envelope. Most mycoviruses are transmitted by cytoplasmic interchange and are thought to lack an extracellular section of their an infection cycle. Structural evaluation has centered on dsRNA mycoviruses, which normally bundle their genome in a 120-subunit T=1 icosahedral capsid, with a capsid protein (CP) dimer because the uneven unit.
The atomic construction is out there for 4 dsRNA mycovirus from completely different households: Saccharomyces cerevisiae virus L-A (ScV-L-A), Penicillium chrysogenum virus (PcV), Penicillium stoloniferum virus F (PsV-F), and Rosellinia necatrix quadrivirus 1 (RnQV1). Their capsids present structural variations of the identical framework, with uneven or symmetric CP dimers respectively for ScV-L-A and PsV-F, dimers of comparable domains of a single CP for PcV, or of two completely different proteins for RnQV1. The CP dimer is the constructing block, and meeting proceeds via dimers of dimers or pentamers of dimers, during which the genome is packed as ssRNA by interplay with CP and/or viral polymerase.
These capsids stay structurally undisturbed all through the viral cycle. The T=1 capsid participates in RNA synthesis, organizing the viral polymerase (1-2 copies) and a single loosely packaged genome phase. It additionally acts as a molecular sieve, to permit the passage of viral transcripts and nucleotides, however to forestall triggering of host protection mechanisms. Because of the shut mycovirus-host relationship, CP developed to allocate peptide insertions with enzyme exercise, as mirrored in a tough outer capsid floor.

Dynamically probing ATP-dependent RHA-assisted RNA construction conversion utilizing single molecule fluorescence resonance power switch

RNA helicase A (RHA) as a member of DExH-box subgroup of helicase superfamily II, participates in numerous organic processes concerned in RNA metabolism in organisms, and these RNA-mediated organic processes depend on RNA construction conversion. Nevertheless, how RHA regulate the RNA construction conversion was nonetheless unknown. To be able to unveil the mechanism of RNA construction conversion mediated by RHA, single molecule fluorescence resonance power switch (smFRET) was adopted to in our assay, and substrates RNA had been from inner ribosome entry web site (IRES) of foot-and-mouth illness virus (FMDV) genome. We first discovered that the RNA construction conversion by RHA towards thermodynamic equilibrium in vitro, and the method of dsRNA YZ transformed to dsRNA XY via a tripartite intermediate state.
As well as, the speed of the RNA construction conversion and the distribution of dsRNA YZ and XY had been affected by ATP concentrations. Our research supplies real-time perception into ATP-dependent RHA-assisted RNA construction conversion on the single molecule stage, the mechanism displayed by RHA could assist in perceive how RHA contributes to many organic features, and the essential mechanistic options illustrated in our work additionally underlay extra complicated protein-assisted RNA construction conversions. This text is protected by copyright. All rights reserved.

Modeling and Predicting RNA Three-Dimensional Buildings

Modeling the three-dimensional construction of RNAs is a milestone towards higher understanding and prediction of nucleic acids molecular features. Physics-based approaches and molecular dynamics simulations are usually not tractable on giant molecules with all-atom fashions. To handle this challenge, coarse-grained fashions of RNA three-dimensional buildings have been developed. On this chapter, we describe a graphical modeling primarily based on the Leontis-Westhof prolonged base pair classification. This illustration of RNA buildings permits us to determine extremely conserved structural motifs with complicated nucleotide interactions in construction databases.
Structure and Expression of Large (+)RNA Genomes of Viruses of Higher Eukaryotes
We present how one can make the most of this information to shortly predict three-dimensional buildings of enormous RNA molecules and current the RNA-MoIP net server (http://rnamoip.cs.mcgill.ca) that streamlines the computational and visualization processes. Lastly, we present current advances within the prediction of native 3D motifs from sequence information with the BayesPairing software program and focus on its affect towards full 3D construction prediction.

Profiling of RNA Construction at Single-Nucleotide Decision Utilizing nextPARS

RNA molecules play essential roles in nearly each mobile course of, and their features are mediated by their sequence and construction. Figuring out the secondary construction of RNAs is central to understanding RNA perform and evolution. RNA construction probing methods coupled to high-throughput sequencing enable figuring out structural options of RNA molecules at transcriptome-wide scales.

Immortalized Mouse Mammary Epithelial Cells (iMMEC)

T0451 1x106 cells / 1.0 ml Ask for price

Salivary Gland Lysate

20-103 0.1 mg
EUR 663.9
Description: Human salivary gland tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human salivary gland tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the salivary gland tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The salivary gland tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Mouse mammary carcinoma Marker-CA153 ELISA Kit

CSB-E08272m-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Mouse mammary carcinoma Marker-CA153 in samples from serum, plasma, homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse mammary carcinoma Marker-CA153 ELISA Kit

1-CSB-E08272m
  • EUR 1135.20
  • EUR 6938.40
  • EUR 3672.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Mouse mammary carcinoma Marker-CA153 in samples from serum, plasma, homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse mammary caFAinoma Marker-CA153 ELISA Kit

CN-02754M1 96T
EUR 549.6

Mouse mammary caFAinoma Marker-CA153 ELISA Kit

CN-02754M2 48T
EUR 368.4

Mouse Mammary carcinoma Aarker-CA153 ELISA Kit

EMM0228 96Tests
EUR 625.2

Mouse mammary carcinoma Marker-CA153 ELISA Kit

GA-E0541MS-48T 48T
EUR 403.2

Mouse mammary carcinoma Marker-CA153 ELISA Kit

GA-E0541MS-96T 96T
EUR 640.8

Mouse mammary carcinoma Marker-CA153)ELISA Kit

QY-E20963 96T
EUR 433.2

Salivary Gland Membrane Lysate

XBL-10846 0.1 mg
EUR 884.4
Description: Human salivary gland tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human salivary gland tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated salivary gland tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated salivary gland tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Immortalized Human Mammary Fibroblasts (HMF3A)

T0153 1x106 cells / 1.0 ml Ask for price

Immortalized Human Mammary Fibroblasts (HMF3S)

T0154 1x106 cells / 1.0 ml Ask for price

Immortalized Human Mammary Epithelial Cells

T0450 1x106 cells / 1.0 ml
EUR 1243.2

Mammary Dissociation System 1 (Adipocytes), Mouse and Rat

4-20321 ea Ask for price

Mammary Dissociation System 2 (Epithelial), Mouse balb/c

4-20322 ea Ask for price

Mammary Dissociation System 4 (Epithelial, Swiss 3T3), Mouse

4-20324 ea Ask for price

Mammary Dissociation System 5 (Epitheliall), Mouse primary tumor

4-20325 ea Ask for price

Mouse Breast PrimaCell: Normal Mammary Epithelial Primary Cells

2-82021 1 Kit Ask for price

Immortalized Mouse Prolactin-Responsive Mammary Epithelial Cells (HC11)

T0672 1x106 cells / 1.0 ml Ask for price

Mouse Submandibular gland protein C, Muc19 ELISA KIT

ELI-35955m 96 Tests
EUR 1038

Immortalized Adult Mouse Cogulating Gland Cells (INK4 -/- CG)

T0656 1x106 cells / 1.0 ml Ask for price

Salivary Gland Tissue Slide (Abnormal)

12-128-10um 10 um
EUR 241.8

Salivary Gland Tissue Slide (Abnormal)

12-128-4um 4 um
EUR 216.6

Pituitary gland, 25 cases (1.1mm)

PIT501 1
EUR 354
Description: Normal pituitary gland tissue array, 25 cases. In most of the cases, two cores were taken, one from anterior lobe and the other from posterior lobe.

Ground Sleeve Gland St20/2

QST20/2 EACH
EUR 118.51

mmu-miRNome MicroRNA Profiling Kit [Mouse]

RA670A-1 20 profiles
EUR 2046

Mouse Mammary carcinoma Marker/Carbohydrate antigen15 3 ELISA kit

E03C0670-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Mammary carcinoma Marker/Carbohydrate antigen15 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Mammary carcinoma Marker/Carbohydrate antigen15 3 ELISA kit

E03C0670-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Mammary carcinoma Marker/Carbohydrate antigen15 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Mammary carcinoma Marker/Carbohydrate antigen15 3 ELISA kit

E03C0670-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Mammary carcinoma Marker/Carbohydrate antigen15 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse FibrOut 1, for adipose, fat, eye, lung, mammary

4-21501 1 ml Ask for price

Mouse FibrOut 1, for adipose, fat, eye, lung, mammary

4-21502 5 x 1 ml Ask for price

Human Mammary carcinoma Marker ELISA kit

E01M0221-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Mammary carcinoma Marker ELISA kit

E01M0221-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Human Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Mammary carcinoma Marker ELISA kit

E01M0221-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Human Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Mammary carcinoma Marker ELISA kit

E06M0221-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Mammary carcinoma Marker ELISA kit

E06M0221-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Goat Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Mammary carcinoma Marker ELISA kit

E06M0221-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Goat Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Mammary carcinoma Marker ELISA kit

E08M0221-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Mammary carcinoma Marker ELISA kit

E08M0221-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Mammary carcinoma Marker ELISA kit

E08M0221-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Mammary carcinoma Marker ELISA kit

E02M0221-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Mammary carcinoma Marker ELISA kit

E02M0221-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Mammary carcinoma Marker ELISA kit

E02M0221-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Mammary carcinoma Marker ELISA kit

E04M0221-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rabbit Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Mammary carcinoma Marker ELISA kit

E04M0221-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rabbit Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Mammary carcinoma Marker ELISA kit

E04M0221-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rabbit Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Mammary carcinoma Marker ELISA kit

E07M0221-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Porcine Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Mammary carcinoma Marker ELISA kit

E07M0221-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Porcine Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Mammary carcinoma Marker ELISA kit

E07M0221-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Porcine Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mammary Dissociation System 6 (Epithelial), Rat

4-20326 ea Ask for price

Breast Tissue Slide (Invasive Mammary Carcinoma)

10-009-10um 10 um
EUR 241.8

Breast Tissue Slide (Invasive Mammary Carcinoma)

10-009-4um 4 um
EUR 216.6

Mammary Serine Protease Inhibitor (Maspin) Antibody

20-abx173450
  • EUR 410.40
  • EUR 1028.40
  • EUR 526.80
  • EUR 184.80
  • EUR 309.60
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Mammary Serine Protease Inhibitor (Maspin) Antibody

20-abx128086
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mammary Serine Protease Inhibitor (SERPINB5) Antibody

20-abx212477
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Mammary Serine Protease Inhibitor (SERPINB5) Antibody

20-abx212546
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Mammary Serine Protease Inhibitor (SERPINB5) Antibody

20-abx329431
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Human Internal Mammary Artery Endothelial Cells

HEC17 500,000+ Cells
EUR 1296

Monkey Mammary carcinoma Marker ELISA kit

E09M0221-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Monkey Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Mammary carcinoma Marker ELISA kit

E09M0221-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Monkey Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Mammary carcinoma Marker ELISA kit

E09M0221-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Monkey Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Recombinant Mammary Serine Protease Inhibitor (Maspin)

4-RPD386Hu01
  • EUR 593.09
  • EUR 282.00
  • EUR 1894.08
  • EUR 711.36
  • EUR 1302.72
  • EUR 472.80
  • EUR 4555.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Mammary Serine Protease Inhibitor expressed in: E.coli

MicroRNA Isolation Kit

KS341025 1 kit
EUR 307.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Mouse Endocrine gland vascular endothelial growth factor ELISA kit

E03E0130-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Mouse Endocrine gland vascular endothelial growth factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Endocrine gland vascular endothelial growth factor ELISA kit

E03E0130-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Mouse Endocrine gland vascular endothelial growth factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Endocrine gland vascular endothelial growth factor ELISA kit

E03E0130-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Mouse Endocrine gland vascular endothelial growth factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse 16.5 kDa submandibular gland glycoprotein, Spt1 ELISA KIT

ELI-29687m 96 Tests
EUR 1038

Mouse Endocrine gland vascular endothelial growth factor, EG- VE

ELA-E0024m 96 Tests
EUR 1038

Mouse mammary tumor virus receptor homolog 1 (MTVR1) polyclonal antibody

ABP-PAB-10111 100 ug Ask for price

Mouse Breast PrimaCell: Normal Mammary Epithelial Primary Cells Growth Medium

9-32021 5 x 100 ml Ask for price

Mouse Breast Tissue Preparation Buffer: Normal Mammary Epithelial Primary Cells

9-80135 1 x 100 ml Ask for price

Pituitary gland adenoma, 45 cases (1.5mm)

PIT481 1
EUR 406.8
Description: Pituitary gland adenoma tissue array, 45 cases including a single core from 42 cases of various types of adenoma and two cores (one from anterior lobe and the other from posterior lobe) from each of three control normal pituitary glands.

Gland With Pulley St 4/2F

QST4/2F EACH
EUR 212.04

Mouse pre-microRNA Expression Construct mir-100

MMIR-100-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-101a

MMIR-101a-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-101b

MMIR-101b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-101c

MMIR-101c-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-105

MMIR-105-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-106a

MMIR-106a-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-106b

MMIR-106b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-107

MMIR-107-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-10a

MMIR-10a-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-10b

MMIR-10b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-122

MMIR-122-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-125a

MMIR-125a-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-126

MMIR-126-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-127

MMIR-127-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-130a

MMIR-130a-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-130b

MMIR-130b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-132

MMIR-132-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-133b

MMIR-133b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-134

MMIR-134-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-135b

MMIR-135b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-136

MMIR-136-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-139

MMIR-139-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-140

MMIR-140-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-141

MMIR-141-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-142

MMIR-142-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-143

MMIR-143-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-145

MMIR-145-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-146a

MMIR-146a-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-146b

MMIR-146b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-148a

MMIR-148a-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-149

MMIR-149-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-150

MMIR-150-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-151

MMIR-151-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-152

MMIR-152-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-153

MMIR-153-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-154

MMIR-154-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-155

MMIR-155-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-181c

MMIR-181c-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-181d

MMIR-181d-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-182

MMIR-182-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-183

MMIR-183-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-184

MMIR-184-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-185

MMIR-185-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-186

MMIR-186-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-187

MMIR-187-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-188

MMIR-188-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-18b

MMIR-18b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-190

MMIR-190-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-190b

MMIR-190b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-191

MMIR-191-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-192

MMIR-192-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-193

MMIR-193-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-193b

MMIR-193b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-195

MMIR-195-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-196b

MMIR-196b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-1b

MMIR-1b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-200a

MMIR-200a-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-200b

MMIR-200b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-200c

MMIR-200c-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-201

MMIR-201-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-202

MMIR-202-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-203

MMIR-203-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-204

MMIR-204-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-205

MMIR-205-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-206

MMIR-206-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-207

MMIR-207-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-208a

MMIR-208a-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-208b

MMIR-208b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-20b

MMIR-20b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-21

MMIR-21-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-210

MMIR-210-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-211

MMIR-211-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-212

MMIR-212-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-214

MMIR-214-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-215

MMIR-215-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-216a

MMIR-216a-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-216b

MMIR-216b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-217

MMIR-217-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-22

MMIR-22-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-221

MMIR-221-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-222

MMIR-222-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-223

MMIR-223-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-224

MMIR-224-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-23b

MMIR-23b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-26b

MMIR-26b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-27b

MMIR-27b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-28

MMIR-28-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-2861

MMIR-2861-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-28b

MMIR-28b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-28c

MMIR-28c-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-291b

MMIR-291b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-296

MMIR-296-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-297b

MMIR-297b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-297c

MMIR-297c-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-298

MMIR-298-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-299

MMIR-299-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-29a

MMIR-29a-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-29c

MMIR-29c-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-300

MMIR-300-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-301a

MMIR-301a-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-301b

MMIR-301b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-302b

MMIR-302b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-30a

MMIR-30a-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-30b

MMIR-30b-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-30d

MMIR-30d-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-30e

MMIR-30e-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-31

MMIR-31-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-32

MMIR-32-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-320

MMIR-320-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-322

MMIR-322-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-323

MMIR-323-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-324

MMIR-324-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-325

MMIR-325-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-326

MMIR-326-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-327

MMIR-327-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-328

MMIR-328-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-329

MMIR-329-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-33

MMIR-33-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-330

MMIR-330-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-331

MMIR-331-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-335

MMIR-335-PA-1 Bacterial Streak
EUR 820.8

Mouse pre-microRNA Expression Construct mir-337

MMIR-337-PA-1 Bacterial Streak
EUR 820.8
Our group lately developed a novel Illumina-based implementation of in vitro parallel probing of RNA buildings referred to as nextPARS.Right here, we describe a protocol for the computation of the nextPARS scores and their use to acquire the structural profile (single- or double-stranded state) of an RNA sequence at single-nucleotide decision.

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