Structure and Expression of Large (+)RNA Genomes of Viruses of Higher Eukaryotes

Structure and Expression of Large (+)RNA Genomes of Viruses of Higher Eukaryotes
Viral positive-sense RNA genomes evolve quickly because of the excessive mutation charges throughout replication and RNA recombination, which permitting the viruses to accumulate and modify genes for his or her adaptation. The dimensions of RNA genome is proscribed by a number of elements, together with low constancy of RNA polymerases and packaging constraints. Nevertheless, the 12-kb measurement restrict is exceeded within the two teams of eukaryotic (+)RNA viruses – animal nidoviruses and plant closteroviruses. These virus teams have a number of traits in frequent.
Their genomes include 5′-proximal genes which might be expressed by way of ribosomal frameshifting and encode one or two papain-like protease domains, membrane-binding area(s), methyltransferase, RNA helicase, and RNA polymerase. As well as, some nidoviruses (i.e., coronaviruses) include replication-associated domains, equivalent to proofreading exonuclease, putative primase, nucleotidyltransferase, and endonuclease. In each nidoviruses and closteroviruses, the three’-terminal a part of the genome incorporates genes for structural and accent proteins expressed by way of a nested set of coterminal subgenomic RNAs.
Coronaviruses and closteroviruses have developed to kind flexuous helically symmetrical nucleocapsids as a imply to resolve packaging constraints. Since phylogenetic reconstructions of the RNA polymerase domains point out solely a marginal relationship between the nidoviruses and closteroviruses, their comparable properties seemingly have developed convergently, together with the rise within the genome measurement.

Construction and meeting of double-stranded RNA mycoviruses

Mycoviruses are a various group that features ssRNA, dsRNA, and ssDNA viruses, with or and not using a protein capsid, in addition to with a posh envelope. Most mycoviruses are transmitted by cytoplasmic interchange and are thought to lack an extracellular section of their an infection cycle. Structural evaluation has centered on dsRNA mycoviruses, which normally bundle their genome in a 120-subunit T=1 icosahedral capsid, with a capsid protein (CP) dimer because the uneven unit.
The atomic construction is out there for 4 dsRNA mycovirus from completely different households: Saccharomyces cerevisiae virus L-A (ScV-L-A), Penicillium chrysogenum virus (PcV), Penicillium stoloniferum virus F (PsV-F), and Rosellinia necatrix quadrivirus 1 (RnQV1). Their capsids present structural variations of the identical framework, with uneven or symmetric CP dimers respectively for ScV-L-A and PsV-F, dimers of comparable domains of a single CP for PcV, or of two completely different proteins for RnQV1. The CP dimer is the constructing block, and meeting proceeds via dimers of dimers or pentamers of dimers, during which the genome is packed as ssRNA by interplay with CP and/or viral polymerase.
These capsids stay structurally undisturbed all through the viral cycle. The T=1 capsid participates in RNA synthesis, organizing the viral polymerase (1-2 copies) and a single loosely packaged genome phase. It additionally acts as a molecular sieve, to permit the passage of viral transcripts and nucleotides, however to forestall triggering of host protection mechanisms. Because of the shut mycovirus-host relationship, CP developed to allocate peptide insertions with enzyme exercise, as mirrored in a tough outer capsid floor.

Dynamically probing ATP-dependent RHA-assisted RNA construction conversion utilizing single molecule fluorescence resonance power switch

RNA helicase A (RHA) as a member of DExH-box subgroup of helicase superfamily II, participates in numerous organic processes concerned in RNA metabolism in organisms, and these RNA-mediated organic processes depend on RNA construction conversion. Nevertheless, how RHA regulate the RNA construction conversion was nonetheless unknown. To be able to unveil the mechanism of RNA construction conversion mediated by RHA, single molecule fluorescence resonance power switch (smFRET) was adopted to in our assay, and substrates RNA had been from inner ribosome entry web site (IRES) of foot-and-mouth illness virus (FMDV) genome. We first discovered that the RNA construction conversion by RHA towards thermodynamic equilibrium in vitro, and the method of dsRNA YZ transformed to dsRNA XY via a tripartite intermediate state.
As well as, the speed of the RNA construction conversion and the distribution of dsRNA YZ and XY had been affected by ATP concentrations. Our research supplies real-time perception into ATP-dependent RHA-assisted RNA construction conversion on the single molecule stage, the mechanism displayed by RHA could assist in perceive how RHA contributes to many organic features, and the essential mechanistic options illustrated in our work additionally underlay extra complicated protein-assisted RNA construction conversions. This text is protected by copyright. All rights reserved.

Modeling and Predicting RNA Three-Dimensional Buildings

Modeling the three-dimensional construction of RNAs is a milestone towards higher understanding and prediction of nucleic acids molecular features. Physics-based approaches and molecular dynamics simulations are usually not tractable on giant molecules with all-atom fashions. To handle this challenge, coarse-grained fashions of RNA three-dimensional buildings have been developed. On this chapter, we describe a graphical modeling primarily based on the Leontis-Westhof prolonged base pair classification. This illustration of RNA buildings permits us to determine extremely conserved structural motifs with complicated nucleotide interactions in construction databases.
Structure and Expression of Large (+)RNA Genomes of Viruses of Higher Eukaryotes
We present how one can make the most of this information to shortly predict three-dimensional buildings of enormous RNA molecules and current the RNA-MoIP net server (http://rnamoip.cs.mcgill.ca) that streamlines the computational and visualization processes. Lastly, we present current advances within the prediction of native 3D motifs from sequence information with the BayesPairing software program and focus on its affect towards full 3D construction prediction.

Profiling of RNA Construction at Single-Nucleotide Decision Utilizing nextPARS

RNA molecules play essential roles in nearly each mobile course of, and their features are mediated by their sequence and construction. Figuring out the secondary construction of RNAs is central to understanding RNA perform and evolution. RNA construction probing methods coupled to high-throughput sequencing enable figuring out structural options of RNA molecules at transcriptome-wide scales.

Mouse C57 Mammary Gland, E14 cDNA

MD-414-14-C57 30 reactions
EUR 280

Mouse CD1 Mammary Gland, E15 cDNA

MD-414-15 30 reactions
EUR 243

Mouse C57 Mammary Gland, E15 cDNA

MD-414-15-C57 30 reactions
EUR 280

Mouse CD1 Mammary Gland, E16 cDNA

MD-414-16 30 reactions
EUR 243

Mouse C57 Mammary Gland, E16 cDNA

MD-414-16-C57 30 reactions
EUR 280

Mouse CD1 Mammary Gland, E17 cDNA

MD-414-17 30 reactions
EUR 243

Mouse C57 Mammary Gland, E17 cDNA

MD-414-17-C57 30 reactions
EUR 280

Mouse CD1 Mammary Gland, E18 cDNA

MD-414-18 30 reactions
EUR 243

Mouse C57 Mammary Gland, E18 cDNA

MD-414-18-C57 30 reactions
EUR 280

Mouse CD1 Mammary Gland Western Blot

MW-414 1 Blot
EUR 668

Mouse C57 Mammary Gland Western Blot

MW-414-C57 1 Blot
EUR 789

Mouse CD1 Mammary Gland, E11 Total RNA

MR-414-11 0.05mg
EUR 160

Mouse C57 Mammary Gland, E11 Total RNA

MR-414-11-C57 0.025mg
EUR 180

Mouse CD1 Mammary Gland, E12 Total RNA

MR-414-12 0.05mg
EUR 160

Mouse C57 Mammary Gland, E12 Total RNA

MR-414-12-C57 0.025mg
EUR 180

Mouse CD1 Mammary Gland, E13 Total RNA

MR-414-13 0.05mg
EUR 160

Mouse C57 Mammary Gland, E13 Total RNA

MR-414-13-C57 0.025mg
EUR 180

Mouse CD1 Mammary Gland, E14 Total RNA

MR-414-14 0.05mg
EUR 160

Mouse C57 Mammary Gland, E14 Total RNA

MR-414-14-C57 0.05mg
EUR 180

Mouse CD1 Mammary Gland, E15 Total RNA

MR-414-15 0.05mg
EUR 160

Mouse C57 Mammary Gland, E15 Total RNA

MR-414-15-C57 0.05mg
EUR 180

Mouse CD1 Mammary Gland, E16 Total RNA

MR-414-16 0.05mg
EUR 160

Mouse C57 Mammary Gland, E16 Total RNA

MR-414-16-C57 0.05mg
EUR 180

Mouse CD1 Mammary Gland, E17 Total RNA

MR-414-17 0.05mg
EUR 160

Mouse C57 Mammary Gland, E17 Total RNA

MR-414-17-C57 0.05mg
EUR 180

Mouse CD1 Mammary Gland, E18 Total RNA

MR-414-18 0.05mg
EUR 160

Mouse C57 Mammary Gland, E18 Total RNA

MR-414-18-C57 0.05mg
EUR 180

Mouse Mammary Gland Developmental Western Blot

MW-414-D 1 Blot
EUR 910

Mouse CD1 Mammary Gland, E11 Total Protein

MT-414-11 0.5mg
EUR 153

Mouse C57 Mammary Gland, E11 Total Protein

MT-414-11-C57 0.5mg
EUR 180

Mouse CD1 Mammary Gland, E12 Total Protein

MT-414-12 0.5mg
EUR 153

Mouse C57 Mammary Gland, E12 Total Protein

MT-414-12-C57 0.5mg
EUR 180

Mouse CD1 Mammary Gland, E13 Total Protein

MT-414-13 0.5mg
EUR 153

Mouse C57 Mammary Gland, E13 Total Protein

MT-414-13-C57 0.5mg
EUR 180

Mouse CD1 Mammary Gland, E14 Total Protein

MT-414-14 0.5mg
EUR 153

Mouse C57 Mammary Gland, E14 Total Protein

MT-414-14-C57 0.5mg
EUR 180

Mouse CD1 Mammary Gland, E15 Total Protein

MT-414-15 0.5mg
EUR 153

Mouse C57 Mammary Gland, E15 Total Protein

MT-414-15-C57 0.5mg
EUR 180

Mouse CD1 Mammary Gland, E16 Total Protein

MT-414-16 0.5mg
EUR 153

Mouse C57 Mammary Gland, E16 Total Protein

MT-414-16-C57 0.5mg
EUR 180

Mouse CD1 Mammary Gland, E17 Total Protein

MT-414-17 0.5mg
EUR 153

Mouse C57 Mammary Gland, E17 Total Protein

MT-414-17-C57 0.5mg
EUR 180

Mouse CD1 Mammary Gland, E18 Total Protein

MT-414-18 0.5mg
EUR 153

Mouse C57 Mammary Gland, E18 Total Protein

MT-414-18-C57 0.5mg
EUR 180

Mouse CD1 Mammary Gland, non pregnant cDNA

MD-414 30 reactions
EUR 243

Mouse CD1 Mammary Gland, non pregnant cDNA

MD-414-1 30 reactions
EUR 243

Mouse BLC Mammary Gland, non pregnant cDNA

MD-414-BLC 30 reactions
EUR 280

Mouse C57 Mammary Gland, non pregnant cDNA

MD-414-C57 30 reactions
EUR 280

Mouse C57 Mammary Gland, non pregnant cDNA

MD-414-C57-1 30 reactions
EUR 280

Mouse C57 Mammary Gland Frozen Sections, E11

MF-414-11-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Frozen Sections, E12

MF-414-12 10 slides
EUR 253

Mouse C57 Mammary Gland Frozen Sections, E12

MF-414-12-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Frozen Sections, E11

MF-414-11 10 slides
EUR 253

Mouse CD1 Mammary Gland Frozen Sections, E13

MF-414-13 10 slides
EUR 253

Mouse C57 Mammary Gland Frozen Sections, E13

MF-414-13-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Frozen Sections, E14

MF-414-14 10 slides
EUR 253

Mouse C57 Mammary Gland Frozen Sections, E14

MF-414-14-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Frozen Sections, E15

MF-414-15 10 slides
EUR 253

Mouse C57 Mammary Gland Frozen Sections, E15

MF-414-15-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Frozen Sections, E16

MF-414-16 10 slides
EUR 253

Mouse C57 Mammary Gland Frozen Sections, E16

MF-414-16-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Frozen Sections, E17

MF-414-17 10 slides
EUR 253

Mouse C57 Mammary Gland Frozen Sections, E17

MF-414-17-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Frozen Sections, E18

MF-414-18 10 slides
EUR 253

Mouse C57 Mammary Gland Frozen Sections, E18

MF-414-18-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, E11

MP-414-11 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, E11

MP-414-11-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, E12

MP-414-12 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, E12

MP-414-12-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, E13

MP-414-13 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, E13

MP-414-13-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, E14

MP-414-14 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, E14

MP-414-14-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, E15

MP-414-15 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, E15

MP-414-15-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, E16

MP-414-16 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, E16

MP-414-16-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, E17

MP-414-17 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, E17

MP-414-17-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, E18

MP-414-18 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, E18

MP-414-18-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Premade Northern Blot

MN-414 1 Blot
EUR 668

Mouse C57 Mammary Gland Premade Northern Blot

MN-414-C57 1 Blot
EUR 903

Mouse CD1 Mammary Gland Frozen Sections, Virgin

MF-414-V 10 slides
EUR 253

Mouse C57 Mammary Gland Frozen Sections, Virgin

MF-414-V-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, Virgin

MP-414-V 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, Virgin

MP-414-V-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland, non pregnant Total RNA

MR-414 0.05mg
EUR 160

Mouse C57 Mammary Gland, non pregnant Total RNA

MR-414-C57 0.025mg
EUR 180

Rabbit Mammary Gland cDNA

TD-414 30 reactions
EUR 243

Mouse Balbc Mammary Gland, non pregnant Total RNA

MR-414-BLC 0.05mg
EUR 180

Mouse CD1 Mammary Gland, non pregnant Total Protein

MT-414 0.5mg
EUR 153

Mouse BLC Mammary Gland, non pregnant Total Protein

MT-414-BLC 0.5mg
EUR 180

Mouse C57 Mammary Gland, non pregnant Total Protein

MT-414-C57 0.5mg
EUR 180

Mouse CD1 Mammary Gland, Day 1 of Lactation cDNA

MD-414-L1 30 reactions
EUR 243

Mouse C57 Mammary Gland, Day 1 of Lactation cDNA

MD-414-L1-C57 30 reactions
EUR 280

Mouse CD1 Mammary Gland, Day 3 of Lactation cDNA

MD-414-L3 30 reactions
EUR 243

Mouse C57 Mammary Gland, Day 3 of Lactation cDNA

MD-414-L3-C57 30 reactions
EUR 280

Mouse CD1 Mammary Gland, Day 7 of Lactation cDNA

MD-414-L7 30 reactions
EUR 243

Mouse C57 Mammary Gland, Day 7 of Lactation cDNA

MD-414-L7-C57 30 reactions
EUR 280

Mouse CD1 Mammary Gland Frozen Sections, Lactation D7

MF-414-L7 10 slides
EUR 253

Mouse C57 Mammary Gland Frozen Sections, Lactation D7

MF-414-L7-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Frozen Sections, Lactation D1

MF-414-L1 10 slides
EUR 253

Mouse C57 Mammary Gland Frozen Sections, Lactation D1

MF-414-L1-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Frozen Sections, Lactation D3

MF-414-L3 10 slides
EUR 253

Mouse C57 Mammary Gland Frozen Sections, Lactation D3

MF-414-L3-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland, Day 1 of Involution cDNA

MD-414-N1 30 reactions
EUR 243

Mouse C57 Mammary Gland, Day 1 of Involution cDNA

MD-414-N1-C57 30 reactions
EUR 280

Mouse CD1 Mammary Gland, Day 7 of Involution cDNA

MD-414-N7 30 reactions
EUR 243

Mouse C57 Mammary Gland, Day 7 of Involution cDNA

MD-414-N7-C57 30 reactions
EUR 280

Mouse CD1 Mammary Gland Frozen Sections, Involution D1

MF-414-N1 10 slides
EUR 253

Mouse C57 Mammary Gland Frozen Sections, Involution D1

MF-414-N1-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Frozen Sections, Involution D7

MF-414-N7 10 slides
EUR 253

Mouse C57 Mammary Gland Frozen Sections, Involution D7

MF-414-N7-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, Lactation D1

MP-414-L1 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, Lactation D1

MP-414-L1-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, Lactation D3

MP-414-L3 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, Lactation D3

MP-414-L3-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, Lactation D7

MP-414-L7 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, Lactation D7

MP-414-L7-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Frozen Sections, Non-Pregnant

MF-414 10 slides
EUR 266

Mouse BLC Mammary Gland Frozen Sections, Non-Pregnant

MF-414-BLC 10 slides
EUR 291

Mouse C57 Mammary Gland Frozen Sections, Non-Pregnant

MF-414-C57 10 slides
EUR 277

Rat Mammary Gland, E12 cDNA

RD-414-12 30 reactions
EUR 243

Rat Mammary Gland, E13 cDNA

RD-414-13 30 reactions
EUR 243

Rat Mammary Gland, E14 cDNA

RD-414-14 30 reactions
EUR 243

Rat Mammary Gland, E15 cDNA

RD-414-15 30 reactions
EUR 243

Rat Mammary Gland, E16 cDNA

RD-414-16 30 reactions
EUR 243

Rat Mammary Gland, E17 cDNA

RD-414-17 30 reactions
EUR 243

Rat Mammary Gland, E18 cDNA

RD-414-18 30 reactions
EUR 243

Rat Mammary Gland, E19 cDNA

RD-414-19 30 reactions
EUR 243

Rat Mammary Gland, E20 cDNA

RD-414-20 30 reactions
EUR 243

Mouse CD1 Mammary Gland Paraffin Sections, Involution D1

MP-414-N1 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, Involution D1

MP-414-N1-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, Involution D7

MP-414-N7 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, Involution D7

MP-414-N7-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, Non-Pregnant

MP-414 10 slides
EUR 266

Mouse BLC Mammary Gland Paraffin Sections, Non-Pregnant

MP-414-BLC 10 slides
EUR 291

Mouse C57 Mammary Gland Paraffin Sections, Non-Pregnant

MP-414-C57 10 slides
EUR 291

Rat Mammary Gland Western Blot

RW-414 1 Blot
EUR 668

Rabbit Mammary Gland Total RNA

TR-414 0.05mg
EUR 160

Mouse CD1 Mammary Gland, Day 1 of Lactation Total RNA

MR-414-L1 0.05mg
EUR 160

Mouse C57 Mammary Gland, Day 1 of Lactation Total RNA

MR-414-L1-C57 0.05mg
EUR 180

Mouse CD1 Mammary Gland, Day 3 of Lactation Total RNA

MR-414-L3 0.05mg
EUR 160

Mouse C57 Mammary Gland, Day 3 of Lactation Total RNA

MR-414-L3-C57 0.05mg
EUR 180

Mouse CD1 Mammary Gland, Day 7 of Lactation Total RNA

MR-414-L7 0.05mg
EUR 160

Mouse C57 Mammary Gland, Day 7 of Lactation Total RNA

MR-414-L7-C57 0.05mg
EUR 180

Mouse C57 Mammary Gland, non pregnant cDNA-Random Primer

MD-414-C57-RH 30 reactions
EUR 280

Mouse CD1 Mammary Gland, non pregnant cDNA-Random Primer

MD-414-HR 30 reactions
EUR 243

Mouse CD1 Mammary Gland, Day 1 of involution Total RNA

MR-414-N1 0.05mg
EUR 160

Mouse C57 Mammary Gland, Day 1 of involution Total RNA

MR-414-N1-C57 0.05mg
EUR 180

Mouse CD1 Mammary Gland, Day 7 of involution Total RNA

MR-414-N7 0.05mg
EUR 160

Mouse C57 Mammary Gland, Day 7 of involution Total RNA

MR-414-N7-C57 0.05mg
EUR 180

Mouse CD1 Mammary Gland, Day 1 of Lactation Total Protein

MT-414-L1 0.5mg
EUR 153

Mouse C57 Mammary Gland, Day 1 of Lactation Total Protein

MT-414-L1-C57 0.5mg
EUR 180

Mouse CD1 Mammary Gland, Day 3 of Lactation Total Protein

MT-414-L3 0.5mg
EUR 153

Mouse C57 Mammary Gland, Day 3 of Lactation Total Protein

MT-414-L3-C57 0.5mg
EUR 180

Mouse CD1 Mammary Gland, Day 7 of Lactation Total Protein

MT-414-L7 0.5mg
EUR 153

Mouse C57 Mammary Gland, Day 7 of Lactation Total Protein

MT-414-L7-C57 0.5mg
EUR 180

Mouse CD1 Mammary Gland, Day 1 of Involution Total Protein

MT-414-N1 0.5mg
EUR 153

Mouse C57 Mammary Gland, Day 1 of Involution Total Protein

MT-414-N1-C57 0.5mg
EUR 180

Mouse CD1 Mammary Gland, Day 7 of Involution Total Protein

MT-414-N7 0.5mg
EUR 153

Mouse C57 Mammary Gland, Day 7 of Involution Total Protein

MT-414-N7-C57 0.5mg
EUR 180

Rat Mammary Gland, E12 Total RNA

RR-414-12 0.05mg
EUR 160

Rat Mammary Gland, E13 Total RNA

RR-414-13 0.05mg
EUR 160

Rat Mammary Gland, E14 Total RNA

RR-414-14 0.05mg
EUR 160

Rat Mammary Gland, E15 Total RNA

RR-414-15 0.05mg
EUR 160

Rat Mammary Gland, E16 Total RNA

RR-414-16 0.05mg
EUR 160

Rat Mammary Gland, E17 Total RNA

RR-414-17 0.05mg
EUR 160

Rat Mammary Gland, E18 Total RNA

RR-414-18 0.05mg
EUR 160

Rat Mammary Gland, E19 Total RNA

RR-414-19 0.05mg
EUR 160

Rat Mammary Gland, E20 Total RNA

RR-414-20 0.05mg
EUR 160

Mouse CD1 Mammary Gland cDNA Panel, set of any 5 Tissues

MD-414-005 5x10 reactions
EUR 867

Mouse C57 Mammary Gland cDNA Panel, set of any 5 Tissues

MD-414-005-C57 5x10 reactions
EUR 1100

Mouse CD1 Mammary Gland cDNA Panel, set of any 10 Tissues

MD-414-010 10x10 reactions
EUR 1158

Mouse C57 Mammary Gland cDNA Panel, set of any 10 Tissues

MD-414-010-C57 10x10 reactions
EUR 1434

Mouse CD1 Mammary Gland cDNA Panel, set of any 14 Tissues

MD-414-014 14x10reactions
EUR 1449

Mouse C57 Mammary Gland cDNA Panel, set of any 14 Tissues

MD-414-014-C57 14x10reactions
EUR 1731

Mouse CD1 mammary gland Frozen Sections Panel, any 15 stages

MF-414-015 15X4slides
EUR 987

Mouse C57 mammary gland Frozen Sections Panel, any 15 stages

MF-414-015-C57 15X4slides
EUR 1109

Mouse CD1 mammary gland Frozen Sections Panel, any 8 tissues

MF-414-008 8X4 slides
EUR 710

Mouse C57 mammary gland Frozen Sections Panel, any 8 tissues

MF-414-008-C57 8X4 slides
EUR 818

Mouse CD1 Mammary Gland Paraffin Sections Panel, any 8 Stages

MP-414-008 8X4 slides
EUR 744

Mouse C57 mammary gland Paraffin Sections Panel, any 8 stages

MP-414-008-C57 8X4 slides
EUR 818

Mouse CD1 Mammary Gland Paraffin Sections Panel, any 15 Stages

MP-414-015 15X4slides
EUR 1035

Mouse C57 mammary gland Paraffin Sections Panel, any 15 stages

MP-414-015-C57 15X4slides
EUR 1109

Guinea Pig Mammary Gland-E20 cDNA

GD-414-20 30 reactions
EUR 280

Guinea Pig Mammary Gland-E25 cDNA

GD-414-25 30 reactions
EUR 280

Guinea Pig Mammary Gland-E35 cDNA

GD-414-35 30 reactions
EUR 280

Guinea Pig Mammary Gland-E40 cDNA

GD-414-40 30 reactions
EUR 280

Rat Mammary Gland, E12 Total Protein

RT-414-12 0.5mg
EUR 153

Rat Mammary Gland, E13 Total Protein

RT-414-13 0.5mg
EUR 153

Rat Mammary Gland, E14 Total Protein

RT-414-14 0.5mg
EUR 153

Rat Mammary Gland, E15 Total Protein

RT-414-15 0.5mg
EUR 153

Rat Mammary Gland, E16 Total Protein

RT-414-16 0.5mg
EUR 153

Rat Mammary Gland, E17 Total Protein

RT-414-17 0.5mg
EUR 153
Our group lately developed a novel Illumina-based implementation of in vitro parallel probing of RNA buildings referred to as nextPARS.Right here, we describe a protocol for the computation of the nextPARS scores and their use to acquire the structural profile (single- or double-stranded state) of an RNA sequence at single-nucleotide decision.

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