The human prototypical SR protein SRSF1 is an oncoprotein that incorporates two RRMs and performs a pivotal function in RNA metabolism. We decided the construction of the RRM1 sure to RNA and located that the area binds preferentially to a CN motif (N is for any nucleotide). Based mostly on this resolution construction, we engineered a protein containing a single glutamate to asparagine mutation (E87N), which good points the power to bind to uridines and thereby prompts SMN exon7 inclusion, a technique that’s used to treatment spinal muscular atrophy.
Lastly, we revealed that the versatile inter-RRM linker of SRSF1 permits RRM1 to bind RNA on each side of RRM2 binding web site. In addition to revealing an sudden bimodal mode of interplay of SRSF1 with RNA, which might be of curiosity to design new therapeutic methods, this examine brings a brand new perspective on the mode of motion of SRSF1 in cells.
Measuring the exercise and construction of purposeful RNAs inside compartments shaped by liquid-liquid part separation
Liquid-liquid part separation (LLPS) has been identified to drive formation of biomolecular compartments, which might encapsulate RNA and proteins amongst different cosolutes. Such compartments, which lack a lipid membrane, have been implicated in origins of life situations as they will simply uptake and focus biomolecules, just like intracellular condensates. Certainly, chemical interactions that drive LLPS in vitro have additionally been proven to result in related sub-cellular compartments in vivo.
Right here we describe strategies to arrange compartments shaped by complicated coacervates, that are pushed by LLPS of oppositely-charged polyions, and to probe the buildings and capabilities of RNAs in them. These strategies might be tailored to check RNA biochemistry in compartments shaped by numerous synthetic and organic macromolecules.
Co-crystal buildings of HIV TAR RNA sure to lab-evolved proteins present key roles for arginine related to the design of cyclic peptide TAR inhibitors
RNA-protein interfaces management key replication occasions through the HIV-1 life cycle. The viral trans-activator of transcription (Tat) protein makes use of an archetypal arginine-rich motif (ARM) to recruit the host optimistic transcription elongation issue b (pTEFb) complicated onto the viral trans-activation response (TAR) RNA, resulting in activation of HIV transcription. Efforts to dam this interplay have stimulated manufacturing of biologics designed to disrupt this important RNA-protein interface. Right here, we current 4 co-crystal buildings of lab-evolved TAR-binding proteins (TBPs) in complicated with HIV-1 TAR. Our outcomes reveal that high-affinity binding requires a definite sequence and spacing of arginines inside a selected β2-β3 hairpin loop that arose throughout choice.
Though loops with as many as 5 arginines had been analyzed, solely three arginines may bind concurrently with major-groove guanines. Amino acids that promote spine interactions inside the β2-β3 loop had been additionally noticed to be vital for high-affinity interactions. Based mostly on structural and affinity analyses, we designed two cyclic peptide mimics of the TAR-binding β2-β3 loop sequences current in two high-affinity TBPs (OkD values of 4.2 ± 0.
Three and three.0 ± 0.Three nm). Our efforts yielded low-molecular weight compounds that bind TAR with low micromolar affinity (OkD values starting from 3.6 to 22 μm). Considerably, one cyclic compound inside this collection blocked binding of the Tat-ARM peptide to TAR in resolution assays, whereas its linear counterpart didn’t. General, this work offers perception into protein-mediated TAR recognition and lays the bottom for the event of cyclic peptide inhibitors of a significant HIV-1 RNA-protein interplay.
Construction of a transcribing RNA polymerase II-U1 snRNP complicated
To provoke cotranscriptional splicing, RNA polymerase II (Pol II) recruits the U1 small nuclear ribonucleoprotein particle (U1 snRNP) to nascent precursor messenger RNA (pre-mRNA). Right here, we report the cryo-electron microscopy construction of a mammalian transcribing Pol II-U1 snRNP complicated. The construction reveals that Pol II and U1 snRNP work together instantly. This interplay positions the pre-mRNA 5′ splice web site close to the RNA exit web site of Pol II.
Extension of pre-mRNA retains the 5′ splice web site, resulting in the formation of a “rising intron loop.” Loop formation could facilitate scanning of nascent pre-mRNA for the three’ splice web site, purposeful pairing of distant intron ends, and prespliceosome meeting. Our outcomes present a place to begin for a mechanistic evaluation of cotranscriptional spliceosome meeting and the biogenesis of mRNA isoforms by different splicing.
Consensus small interfering RNA focused to stem-loops II and III of IRES construction of 5′ UTR successfully inhibits virus replication and translation of HCV sub-genotype 4a isolates from Saudi Arabia
Being probably the most conserved area of all hepatitis C virus (HCV) genotypes and sub-genotypes, the 5′ untranslated area (5′ UTR) of HCV genome signifies it is significance as a possible goal for anti-mRNA based mostly remedy methods like RNA interference. The arrival and approval of first small interference RNA (siRNA) -based remedy of hereditary transthyretin-mediated amyloidosis for scientific use has raised the hopes to check this method towards extremely prone viruses like HCV. We investigated the antiviral potential of consensus siRNAs focused to stem-loops (SLs) II and III nucleotide motifs of inside ribosome entry web site (IRES) construction inside 5′ UTR of HCV sub-genotype 4a isolates from the Saudi inhabitants. siRNA inhibitory results on viral replication and translation of full-length HCV genome had been decided in a reliable, persistent, and reproducible Huh-7 cell tradition system maintained for one month.
Maximal inhibition of RNA transcript ranges of HCV-IRES clones and silencing of viral replication and translation of full-length virus genome was demonstrated by siRNAs focused to SL-III nucleotide motifs of IRES in Huh-7 cells. siRNA Usi-169 decreased 5′ UTR RNA transcript ranges of HCV-IRES clones as much as 75% (P < 0.001) at 24 h post-transfection and 80% (P < 0.001) at 48 h remedy in Huh-7 cells. 5′ UTR-tagged GFP protein expression was considerably decreased from 70 to 80% in Huh-7 cells co-transfected with constructed vectors (i.e. pCR3.1/GFP/5′ UTR) and siRNA Usi-169 at 24 h and 48 h time-span. Viral replication was inhibited by greater than 90% (P < 0.001) and HCV core (C) and hypervariable envelope glycoproteins (E1 and E2) expression was additionally considerably degraded by intracytoplasmic siRNA Usi-169 exercise in persistent Huh-7 cell tradition system.
 Human Troponin C, slow skeletal and cardiac muscles, TNNC1 ELISA |
|
ELI-40072h |
Lifescience Market |
96 Tests |
EUR 988.8 |
 Bovine Troponin C, slow skeletal and cardiac muscles, TNNC1 ELIS |
|
ELI-17222b |
Lifescience Market |
96 Tests |
EUR 1113.6 |
 ELISA Kit) Pig Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit |
|
abx555672-96tests |
Abbexa |
96 tests |
EUR 1093.2 |
|
|
 ELISA Kit) Cow Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit |
|
abx555769-96tests |
Abbexa |
96 tests |
EUR 1093.2 |
|
|
 ELISA kit for Human Troponin C, slow skeletal and cardiac muscles |
|
EK3670 |
SAB |
96 tests |
EUR 804 |
|
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Troponin C, slow skeletal and cardiac muscles in samples from serum, plasma, tissue homogenates and other biological fluids. |
 ELISA Kit) Human Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit |
|
abx352187-96tests |
Abbexa |
96 tests |
EUR 943.2 |
|
|
Human Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit |
|
abx555435-96tests |
Abbexa |
96 tests |
EUR 943.2 |
|
|
 ELISA Kit) Mouse Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit |
|
abx555608-96tests |
Abbexa |
96 tests |
EUR 886.8 |
|
|
Human Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit |
|
20-abx153332 |
Abbexa |
-
EUR 8853.60
-
EUR 4719.60
-
EUR 1093.20
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
|
 Human TNNC1/ Troponin C, slow skeletal and cardiac muscles ELISA Kit |
|
E2558Hu |
Sunlong |
1 Kit |
EUR 726 |
 ELISA Kit) Chicken Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit |
|
abx555347-96tests |
Abbexa |
96 tests |
EUR 1093.2 |
|
|
) ELISA kit for Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) |
|
KTE50026-48T |
Abbkine |
48T |
EUR 510 |
|
Description: Quantitative sandwich ELISA for measuring Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) |
|
KTE50026-5platesof96wells |
Abbkine |
5 plates of 96 wells |
EUR 3631.2 |
|
Description: Quantitative sandwich ELISA for measuring Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) |
|
KTE50026-96T |
Abbkine |
96T |
EUR 907.2 |
|
Description: Quantitative sandwich ELISA for measuring Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
) ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) |
|
KTE70102-48T |
Abbkine |
48T |
EUR 398.4 |
|
|
|
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) |
|
KTE70102-5platesof96wells |
Abbkine |
5 plates of 96 wells |
EUR 2538 |
|
|
|
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) |
|
KTE70102-96T |
Abbkine |
96T |
EUR 646.8 |
|
|
|
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
) ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) |
|
KTE30009-48T |
Abbkine |
48T |
EUR 424.8 |
|
|
|
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) |
|
KTE30009-5platesof96wells |
Abbkine |
5 plates of 96 wells |
EUR 2702.4 |
|
|
|
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) |
|
KTE30009-96T |
Abbkine |
96T |
EUR 686.4 |
|
|
|
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
 Rat Skeletal Muscle Tissue Lysate |
|
LYSATE0010 |
BosterBio |
200ug |
EUR 180 |
|
Description: This cell lysate is prepared from rat skeletal muscle tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 cDNA - Rat Normal Tissue: Skeletal Muscle |
|
C1434171 |
Biochain |
40 reactions |
EUR 385 |
 Skeletal Muscle Lysate |
|
1467 |
ProSci |
0.1 mg |
EUR 229.2 |
|
Description: Skeletal Muscle tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Skeletal Muscle Lysate |
|
21-107 |
ProSci |
0.1 mg |
EUR 342.6 |
|
Description: Bovine skeletal muscle tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The bovine skeletal muscle tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the skeletal muscle tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The skeletal muscle tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Skeletal Muscle Lysate |
|
21-197 |
ProSci |
0.1 mg |
EUR 342.6 |
|
Description: Monkey (Cynomolgus) skeletal muscle tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) skeletal muscle tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the skeletal muscle tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The skeletal muscle tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
 Rat Muscle PrimaCell1: Normal Skeletal Muscle Cells |
|
2-82573 |
CHI Scientific |
1 Kit |
Ask for price |
 Rat Skeletal muscle Actinin Alpha ELISA kit |
|
E02S0267-192T |
BlueGene |
192 tests |
EUR 1524 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Skeletal muscle Actinin Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Skeletal muscle Actinin Alpha ELISA kit |
|
E02S0267-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Skeletal muscle Actinin Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Skeletal muscle Actinin Alpha ELISA kit |
|
E02S0267-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Skeletal muscle Actinin Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 Total RNA - Rat Normal Tissue: Skeletal Muscle |
|
R1434171-50 |
Biochain |
50 ug |
EUR 130 |
 Troponin-I Rat Skeletal Muscle, pure protein |
|
TNIM50-N-25 |
Alpha Diagnostics |
25 ug |
EUR 270 |
 Rat Actin Alpha 1, Skeletal Muscle ELISA kit |
|
E02A0391-192T |
BlueGene |
192 tests |
EUR 1524 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Actin Alpha 1, Skeletal Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Actin Alpha 1, Skeletal Muscle ELISA kit |
|
E02A0391-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Actin Alpha 1, Skeletal Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Actin Alpha 1, Skeletal Muscle ELISA kit |
|
E02A0391-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Actin Alpha 1, Skeletal Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 Genomic DNA - Rat Normal Tissue: Skeletal Muscle |
|
D1434171 |
Biochain |
100 ug |
EUR 286 |
 Skeletal Muscle Actin Antibody |
|
F40313-0.4ML |
NSJ Bioreagents |
0.4 ml |
EUR 330.65 |
|
Description: The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with a-actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an a-actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. |
 ELISA kit) Rat Actin, Alpha skeletal muscle(ACTA1) ELISA kit |
|
E02A1218-192T |
BlueGene |
192 tests |
EUR 1524 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Actin, Alpha skeletal muscle(ACTA1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Actin, Alpha skeletal muscle(ACTA1) ELISA kit |
|
E02A1218-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Actin, Alpha skeletal muscle(ACTA1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Actin, Alpha skeletal muscle(ACTA1) ELISA kit |
|
E02A1218-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Actin, Alpha skeletal muscle(ACTA1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 Skeletal Muscle Membrane Lysate |
|
XBL-10852 |
ProSci |
0.1 mg |
EUR 619.8 |
|
Description: human skeletal muscle tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human skeletal muscle tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated Skeletal Muscle tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated Skeletal Muscle tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
 ELISA Kit) Rat Actin Alpha 1, Skeletal Muscle (ACTA1) ELISA Kit |
|
abx571081-96tests |
Abbexa |
96 tests |
EUR 943.2 |
|
|
Rat Actin Alpha 1, Skeletal Muscle (ACTA1) ELISA Kit |
|
abx354231-96tests |
Abbexa |
96 tests |
EUR 943.2 |
|
|
 ELISA Kit) Rat Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit |
|
DLR-ACTa1-Ra-48T |
DL Develop |
48T |
EUR 633.6 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Actin Alpha 1, Skeletal Muscle (ACTa1) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Rat Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit |
|
DLR-ACTa1-Ra-96T |
DL Develop |
96T |
EUR 828 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Actin Alpha 1, Skeletal Muscle (ACTa1) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
)ELISA Kit) Rat Actin Alpha 1(Skeletal Muscle(ACTa1))ELISA Kit |
|
GA-E0664RT-48T |
GenAsia Biotech |
48T |
EUR 424.8 |
Rat Actin Alpha 1(Skeletal Muscle(ACTa1))ELISA Kit |
|
GA-E0664RT-96T |
GenAsia Biotech |
96T |
EUR 685.2 |
 ELISA kit for Rat Troponin T, fast skeletal muscle |
|
EK3859 |
SAB |
96 tests |
EUR 663.6 |
|
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Troponin T, fast skeletal muscle in samples from serum, plasma, tissue homogenates and other biological fluids. |
 ELISA kit for Rat Troponin T, slow skeletal muscle |
|
EK3993 |
SAB |
96 tests |
EUR 663.6 |
|
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Troponin T, slow skeletal muscle in samples from serum, plasma, tissue homogenates and other biological fluids. |
 ELISA kit for Rat Troponin I, slow skeletal muscle |
|
EK4331 |
SAB |
96 tests |
EUR 582 |
|
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Troponin I, slow skeletal muscle in samples from serum, plasma, tissue homogenates and other biological fluids. |
Rat Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit |
|
RD-ACTa1-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 640.8 |
Rat Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit |
|
RD-ACTa1-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 890.4 |
Rat Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit |
|
RDR-ACTa1-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 669.6 |
Rat Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit |
|
RDR-ACTa1-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 931.2 |
Rat Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit |
|
RD-ACTa1-Ra-48T |
Reddot Biotech |
48T |
EUR 465.5 |
|
|
|
Description: tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. |
Rat Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit |
|
RD-ACTa1-Ra-96T |
Reddot Biotech |
96T |
EUR 665 |
|
|
|
Description: tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. |
Rat Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit |
|
RDR-ACTa1-Ra-48T |
Reddot Biotech |
48T |
EUR 488.78 |
|
|
|
Description: tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. |
Rat Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit |
|
RDR-ACTa1-Ra-96T |
Reddot Biotech |
96T |
EUR 698.25 |
|
|
|
Description: tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. |
 (Mouse)) alpha Actin (Skeletal Muscle) (Mouse) |
|
30R-3408 |
Fitzgerald |
500 ug |
EUR 1701.6 |
|
Description: Recombinant Mouse Actin, alpha skeletal muscle(Acta1) |
 Skeletal Muscle Cytoplasmic Lysate |
|
XBL-10851 |
ProSci |
0.1 mg |
EUR 273.3 |
|
Description: human skeletal muscle tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human skeletal muscle tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated Skeletal Muscle tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated Skeletal Muscle tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot. |
 Rat Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Medium |
|
9-25073 |
CHI Scientific |
5 x 100 ml |
Ask for price |
 (Rabbit)) alpha Actin (Skeletal Muscle) (Rabbit) |
|
30R-3107 |
Fitzgerald |
1 mg |
EUR 308.4 |
|
Description: Purified native alpha Actin (Skeletal Muscle) (Rabbit) |
 ELISA Kit) Rat Troponin I, Fast Skeletal Muscle (TNNI2) ELISA Kit |
|
abx256153-96tests |
Abbexa |
96 tests |
EUR 895.2 |
|
|
 ELISA Kit) Rat Troponin I, Slow Skeletal Muscle (TNNI1) ELISA Kit |
|
abx256154-96tests |
Abbexa |
96 tests |
EUR 895.2 |
|
|
 ELISA Kit) Rat Troponin T, Fast Skeletal Muscle (TNNT3) ELISA Kit |
|
abx256554-96tests |
Abbexa |
96 tests |
EUR 848.4 |
|
|
 ELISA Kit) Rat Troponin T, Slow Skeletal Muscle (TNNT1) ELISA Kit |
|
abx256573-96tests |
Abbexa |
96 tests |
EUR 848.4 |
|
|
Rat Troponin T, Slow Skeletal Muscle (TNNT1) ELISA Kit |
|
abx575017-96tests |
Abbexa |
96 tests |
EUR 801.6 |
|
|
Rat Troponin I, Fast Skeletal Muscle (TNNI2) ELISA Kit |
|
abx575078-96tests |
Abbexa |
96 tests |
EUR 801.6 |
|
|
Rat Troponin I, Slow Skeletal Muscle (TNNI1) ELISA Kit |
|
abx575636-96tests |
Abbexa |
96 tests |
EUR 801.6 |
|
|
Rat Troponin T, Fast Skeletal Muscle (TNNT3) ELISA Kit |
|
abx518123-96tests |
Abbexa |
96 tests |
EUR 848.4 |
|
|
 ELISA kit) Rat Troponin I, slow skeletal muscle(TNNI1) ELISA kit |
|
CSB-EL024011RA-24T |
Cusabio |
1 plate of 24 wells |
EUR 198 |
|
|
|
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin I, slow skeletal muscle (TNNI1) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Rat Troponin I, slow skeletal muscle(TNNI1) ELISA kit |
|
1-CSB-EL024011RA |
Cusabio |
-
EUR 843.60
-
EUR 5811.60
-
EUR 3084.00
|
- 1 plate of 96 wells
- 10 plates of 96 wells each
- 5 plates of 96 wells each
|
|
|
|
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin I, slow skeletal muscle(TNNI1) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
 ELISA kit) Rat Troponin I, fast skeletal muscle(TNNI2) ELISA kit |
|
CSB-EL024012RA-24T |
Cusabio |
1 plate of 24 wells |
EUR 198 |
|
|
|
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin I, fast skeletal muscle (TNNI2) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Rat Troponin I, fast skeletal muscle(TNNI2) ELISA kit |
|
1-CSB-EL024012RA |
Cusabio |
-
EUR 964.80
-
EUR 6118.80
-
EUR 3244.80
|
- 1 plate of 96 wells
- 10 plates of 96 wells each
- 5 plates of 96 wells each
|
|
|
|
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin I, fast skeletal muscle(TNNI2) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
 ELISA kit) Rat Troponin T, slow skeletal muscle(TNNT1) ELISA kit |
|
CSB-EL024015RA-24T |
Cusabio |
1 plate of 24 wells |
EUR 198 |
|
|
|
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin T, slow skeletal muscle (TNNT1) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Rat Troponin T, slow skeletal muscle(TNNT1) ELISA kit |
|
1-CSB-EL024015RA |
Cusabio |
-
EUR 964.80
-
EUR 6118.80
-
EUR 3244.80
|
- 1 plate of 96 wells
- 10 plates of 96 wells each
- 5 plates of 96 wells each
|
|
|
|
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin T, slow skeletal muscle(TNNT1) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
 ELISA kit) Rat Troponin T, fast skeletal muscle(TNNT3) ELISA kit |
|
CSB-EL024017RA-24T |
Cusabio |
1 plate of 24 wells |
EUR 198 |
|
|
|
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin T, fast skeletal muscle (TNNT3) in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Rat Troponin T, fast skeletal muscle(TNNT3) ELISA kit |
|
1-CSB-EL024017RA |
Cusabio |
-
EUR 964.80
-
EUR 6118.80
-
EUR 3244.80
|
- 1 plate of 96 wells
- 10 plates of 96 wells each
- 5 plates of 96 wells each
|
|
|
|
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin T, fast skeletal muscle(TNNT3) in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
Rat Troponin I, Slow Skeletal Muscle (TNNI1) ELISA Kit |
|
20-abx156169 |
Abbexa |
-
EUR 8684.40
-
EUR 4626.00
-
EUR 1074.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
|
Rat Troponin I, Fast Skeletal Muscle (TNNI2) ELISA Kit |
|
20-abx156170 |
Abbexa |
-
EUR 8684.40
-
EUR 4626.00
-
EUR 1074.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
|
Rat Troponin T, Slow Skeletal Muscle (TNNT1) ELISA Kit |
|
20-abx156172 |
Abbexa |
-
EUR 8684.40
-
EUR 4626.00
-
EUR 1074.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
|
 ELISA Kit) Rat Tnni2(Troponin I, fast skeletal muscle) ELISA Kit |
|
ER0168 |
FN Test |
96T |
EUR 681.12 |
|
|
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 18.75pg/ml |
 ELISA Kit) Rat Tnni1(Troponin I, slow skeletal muscle) ELISA Kit |
|
ER0169 |
FN Test |
96T |
EUR 681.12 |
|
|
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 18.75pg/ml |
 ELISA Kit) Rat Tnnt3(Troponin T, fast skeletal muscle) ELISA Kit |
|
ER0579 |
FN Test |
96T |
EUR 628.92 |
|
|
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 18.75pg/ml |
 ELISA Kit) Rat Tnnt1(Troponin T, slow skeletal muscle) ELISA Kit |
|
ER0598 |
FN Test |
96T |
EUR 628.92 |
|
|
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 9.375pg/ml |
 Rat Tnni1/ Troponin I, slow skeletal muscle ELISA Kit |
|
E0992Ra |
Sunlong |
1 Kit |
EUR 666 |
 Rat Tnni2/ Troponin I, fast skeletal muscle ELISA Kit |
|
E0993Ra |
Sunlong |
1 Kit |
EUR 666 |
 Rat Tnnt1/ Troponin T, slow skeletal muscle ELISA Kit |
|
E0995Ra |
Sunlong |
1 Kit |
EUR 685.2 |
 Rat Tnnt3/ Troponin T, fast skeletal muscle ELISA Kit |
|
E0997Ra |
Sunlong |
1 Kit |
EUR 685.2 |
Rat Troponin I, fast skeletal muscle(TNNI2) ELISA kit |
|
E02T0719-192T |
BlueGene |
192 tests |
EUR 1524 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Troponin I, fast skeletal muscle(TNNI2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Troponin I, fast skeletal muscle(TNNI2) ELISA kit |
|
E02T0719-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Troponin I, fast skeletal muscle(TNNI2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Troponin I, fast skeletal muscle(TNNI2) ELISA kit |
|
E02T0719-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Troponin I, fast skeletal muscle(TNNI2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Troponin T, fast skeletal muscle(TNNT3) ELISA kit |
|
E02T0722-192T |
BlueGene |
192 tests |
EUR 1524 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Troponin T, fast skeletal muscle(TNNT3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Troponin T, fast skeletal muscle(TNNT3) ELISA kit |
|
E02T0722-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Troponin T, fast skeletal muscle(TNNT3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Troponin T, fast skeletal muscle(TNNT3) ELISA kit |
|
E02T0722-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Troponin T, fast skeletal muscle(TNNT3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 ELISA Kit) Rat Troponin T, slow skeletal muscle(TNNT1) ELISA Kit |
|
YLA1210RA-48T |
Shanghai YL Biotech |
48T |
EUR 465 |
Rat Troponin T, slow skeletal muscle(TNNT1) ELISA Kit |
|
YLA1210RA-96T |
Shanghai YL Biotech |
96T |
EUR 600 |
 ELISA Kit) Rat Troponin T, slow skeletal muscle(TNNT3) ELISA Kit |
|
YLA1211RA-48T |
Shanghai YL Biotech |
48T |
EUR 465 |
Rat Troponin T, slow skeletal muscle(TNNT3) ELISA Kit |
|
YLA1211RA-96T |
Shanghai YL Biotech |
96T |
EUR 600 |
 ELISA Kit) Rat Troponin I, fast skeletal muscle(TNNI2) ELISA Kit |
|
YLA1212RA-48T |
Shanghai YL Biotech |
48T |
EUR 465 |
Rat Troponin I, fast skeletal muscle(TNNI2) ELISA Kit |
|
YLA1212RA-96T |
Shanghai YL Biotech |
96T |
EUR 600 |
) Troponin T protein (Skeletal Muscle) |
|
30R-AT042 |
Fitzgerald |
100 ug |
EUR 969.6 |
|
Description: Purified native Human Troponin T protein (Skeletal Muscle) |
) Troponin I protein (Skeletal Muscle) |
|
30R-AT060 |
Fitzgerald |
100 ug |
EUR 1412.4 |
|
Description: Purified native Troponin I protein (Skeletal Muscle) |
) Troponin I protein (Skeletal muscle) |
|
30-AT48 |
Fitzgerald |
100 ug |
EUR 706.8 |
|
Description: Purified native Human Troponin I protein (Skeletal muscle) |
 Rabbit Skeletal Muscle Troponin-C |
|
7-06205 |
CHI Scientific |
1mg |
Ask for price |
Rabbit Skeletal Muscle Troponin-C |
|
7-06206 |
CHI Scientific |
5mg |
Ask for price |
Rabbit Skeletal Muscle Troponin-C |
|
7-06207 |
CHI Scientific |
10mg |
Ask for price |
 Mouse Skeletal Muscle Tissue Lysate |
|
LYSATE0021 |
BosterBio |
200ug |
EUR 180 |
|
Description: This cell lysate is prepared from mouse skeletal muscle tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 Rat Troponin I Type 1,Slow Skeletal muscle ELISA kit |
|
E02T0878-192T |
BlueGene |
192 tests |
EUR 1524 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Rat Troponin I Type 1,Slow Skeletal muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Troponin I Type 1,Slow Skeletal muscle ELISA kit |
|
E02T0878-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Rat Troponin I Type 1,Slow Skeletal muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Troponin I Type 1,Slow Skeletal muscle ELISA kit |
|
E02T0878-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Rat Troponin I Type 1,Slow Skeletal muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 Rat skeletal muscle Actinin-α,sm Actinin-α ELISA Kit |
|
CN-01902R1 |
ChemNorm |
96T |
EUR 586.8 |
Rat skeletal muscle Actinin-α,sm Actinin-α ELISA Kit |
|
CN-01902R2 |
ChemNorm |
48T |
EUR 405.6 |
ELISA Kit) Rat skeletal muscle Actinin-α(sm Actinin-α)ELISA Kit |
|
GA-E0300RT-48T |
GenAsia Biotech |
48T |
EUR 380.4 |
Rat skeletal muscle Actinin-α(sm Actinin-α)ELISA Kit |
|
GA-E0300RT-96T |
GenAsia Biotech |
96T |
EUR 595.2 |
Rat skeletal muscle Actinin-α(sm Actinin-α)ELISA Kit |
|
QY-E11352 |
Qayee Biotechnology |
96T |
EUR 433.2 |
Rat skeletal muscle Actinin-α,sm Actinin-α ELISA Kit |
|
YLA0895RA-48T |
Shanghai YL Biotech |
48T |
EUR 465 |
Rat skeletal muscle Actinin-α,sm Actinin-α ELISA Kit |
|
YLA0895RA-96T |
Shanghai YL Biotech |
96T |
EUR 600 |
) Troponin I antibody (Skeletal Muscle) |
|
10-T81B |
Fitzgerald |
1 mg |
EUR 603.6 |
|
Description: Mouse monoclonal Troponin I antibody (Skeletal Muscle) |
Troponin I antibody (Skeletal Muscle) |
|
10-T126C |
Fitzgerald |
1 mg |
EUR 562.8 |
|
Description: Mouse monoclonal Troponin I antibody (Skeletal Muscle) |
Troponin I antibody (Skeletal Muscle) |
|
10R-T126a |
Fitzgerald |
1 mg |
EUR 681.6 |
|
Description: Mouse monoclonal Troponin I antibody (Skeletal Muscle) |
Troponin I antibody (Skeletal Muscle) |
|
10R-T126b |
Fitzgerald |
1 mg |
EUR 681.6 |
|
Description: Mouse monoclonal Troponin I antibody (Skeletal Muscle) |
Troponin I antibody (Skeletal Muscle) |
|
10R-T126c |
Fitzgerald |
1 mg |
EUR 681.6 |
|
Description: Mouse monoclonal Troponin I antibody (Skeletal Muscle) |
The findings unveil that siRNAs focused to five’ UTR-IRES of HCV sub-genotype 4a Saudi isolates present potent silencing of HCV replication and blocking of viral translation in a persistent in-vitro Huh-7 tissue tradition system. Moreover, we additionally elucidated that siRNA silencing of viral mRNA not solely inhibits viral replication but in addition blocks viral translation. The outcomes recommend that siRNA potent antiviral exercise needs to be thought-about as an efficient anti-mRNA based mostly remedy methods for additional in-vivo investigations towards much less studied and harder-to-treat HCV sub-genotype 4a isolates in Saudi Arabia.
