The human prototypical SR protein SRSF1 is an oncoprotein that incorporates two RRMs and performs a pivotal function in RNA metabolism. We decided the construction of the RRM1 sure to RNA and located that the area binds preferentially to a CN motif (N is for any nucleotide). Based mostly on this resolution construction, we engineered a protein containing a single glutamate to asparagine mutation (E87N), which good points the power to bind to uridines and thereby prompts SMN exon7 inclusion, a technique that’s used to treatment spinal muscular atrophy.
Lastly, we revealed that the versatile inter-RRM linker of SRSF1 permits RRM1 to bind RNA on each side of RRM2 binding web site. In addition to revealing an sudden bimodal mode of interplay of SRSF1 with RNA, which might be of curiosity to design new therapeutic methods, this examine brings a brand new perspective on the mode of motion of SRSF1 in cells.
Measuring the exercise and construction of purposeful RNAs inside compartments shaped by liquid-liquid part separation
Liquid-liquid part separation (LLPS) has been identified to drive formation of biomolecular compartments, which might encapsulate RNA and proteins amongst different cosolutes. Such compartments, which lack a lipid membrane, have been implicated in origins of life situations as they will simply uptake and focus biomolecules, just like intracellular condensates. Certainly, chemical interactions that drive LLPS in vitro have additionally been proven to result in related sub-cellular compartments in vivo.
Right here we describe strategies to arrange compartments shaped by complicated coacervates, that are pushed by LLPS of oppositely-charged polyions, and to probe the buildings and capabilities of RNAs in them. These strategies might be tailored to check RNA biochemistry in compartments shaped by numerous synthetic and organic macromolecules.
Co-crystal buildings of HIV TAR RNA sure to lab-evolved proteins present key roles for arginine related to the design of cyclic peptide TAR inhibitors
RNA-protein interfaces management key replication occasions through the HIV-1 life cycle. The viral trans-activator of transcription (Tat) protein makes use of an archetypal arginine-rich motif (ARM) to recruit the host optimistic transcription elongation issue b (pTEFb) complicated onto the viral trans-activation response (TAR) RNA, resulting in activation of HIV transcription. Efforts to dam this interplay have stimulated manufacturing of biologics designed to disrupt this important RNA-protein interface. Right here, we current 4 co-crystal buildings of lab-evolved TAR-binding proteins (TBPs) in complicated with HIV-1 TAR. Our outcomes reveal that high-affinity binding requires a definite sequence and spacing of arginines inside a selected β2-β3 hairpin loop that arose throughout choice.
Though loops with as many as 5 arginines had been analyzed, solely three arginines may bind concurrently with major-groove guanines. Amino acids that promote spine interactions inside the β2-β3 loop had been additionally noticed to be vital for high-affinity interactions. Based mostly on structural and affinity analyses, we designed two cyclic peptide mimics of the TAR-binding β2-β3 loop sequences current in two high-affinity TBPs (OkD values of 4.2 ± 0.
Three and three.0 ± 0.Three nm). Our efforts yielded low-molecular weight compounds that bind TAR with low micromolar affinity (OkD values starting from 3.6 to 22 μm). Considerably, one cyclic compound inside this collection blocked binding of the Tat-ARM peptide to TAR in resolution assays, whereas its linear counterpart didn’t. General, this work offers perception into protein-mediated TAR recognition and lays the bottom for the event of cyclic peptide inhibitors of a significant HIV-1 RNA-protein interplay.
Construction of a transcribing RNA polymerase II-U1 snRNP complicated
To provoke cotranscriptional splicing, RNA polymerase II (Pol II) recruits the U1 small nuclear ribonucleoprotein particle (U1 snRNP) to nascent precursor messenger RNA (pre-mRNA). Right here, we report the cryo-electron microscopy construction of a mammalian transcribing Pol II-U1 snRNP complicated. The construction reveals that Pol II and U1 snRNP work together instantly. This interplay positions the pre-mRNA 5′ splice web site close to the RNA exit web site of Pol II.
Extension of pre-mRNA retains the 5′ splice web site, resulting in the formation of a “rising intron loop.” Loop formation could facilitate scanning of nascent pre-mRNA for the three’ splice web site, purposeful pairing of distant intron ends, and prespliceosome meeting. Our outcomes present a place to begin for a mechanistic evaluation of cotranscriptional spliceosome meeting and the biogenesis of mRNA isoforms by different splicing.
Consensus small interfering RNA focused to stem-loops II and III of IRES construction of 5′ UTR successfully inhibits virus replication and translation of HCV sub-genotype 4a isolates from Saudi Arabia
Being probably the most conserved area of all hepatitis C virus (HCV) genotypes and sub-genotypes, the 5′ untranslated area (5′ UTR) of HCV genome signifies it is significance as a possible goal for anti-mRNA based mostly remedy methods like RNA interference. The arrival and approval of first small interference RNA (siRNA) -based remedy of hereditary transthyretin-mediated amyloidosis for scientific use has raised the hopes to check this method towards extremely prone viruses like HCV. We investigated the antiviral potential of consensus siRNAs focused to stem-loops (SLs) II and III nucleotide motifs of inside ribosome entry web site (IRES) construction inside 5′ UTR of HCV sub-genotype 4a isolates from the Saudi inhabitants. siRNA inhibitory results on viral replication and translation of full-length HCV genome had been decided in a reliable, persistent, and reproducible Huh-7 cell tradition system maintained for one month.
Maximal inhibition of RNA transcript ranges of HCV-IRES clones and silencing of viral replication and translation of full-length virus genome was demonstrated by siRNAs focused to SL-III nucleotide motifs of IRES in Huh-7 cells. siRNA Usi-169 decreased 5′ UTR RNA transcript ranges of HCV-IRES clones as much as 75% (P < 0.001) at 24 h post-transfection and 80% (P < 0.001) at 48 h remedy in Huh-7 cells. 5′ UTR-tagged GFP protein expression was considerably decreased from 70 to 80% in Huh-7 cells co-transfected with constructed vectors (i.e. pCR3.1/GFP/5′ UTR) and siRNA Usi-169 at 24 h and 48 h time-span. Viral replication was inhibited by greater than 90% (P < 0.001) and HCV core (C) and hypervariable envelope glycoproteins (E1 and E2) expression was additionally considerably degraded by intracytoplasmic siRNA Usi-169 exercise in persistent Huh-7 cell tradition system.
 Human Troponin C, slow skeletal and cardiac muscles, TNNC1 ELISA |
|
ELI-40072h |
Lifescience Market |
96 Tests |
EUR 824 |
 Bovine Troponin C, slow skeletal and cardiac muscles, TNNC1 ELIS |
|
ELI-17222b |
Lifescience Market |
96 Tests |
EUR 928 |
 ELISA Kit) Human Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit |
|
20-abx153332 |
Abbexa |
-
EUR 7378.00
-
EUR 3933.00
-
EUR 911.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
|
 ELISA Kit) Chicken Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit |
|
abx555347-96tests |
Abbexa |
96 tests |
EUR 911 |
|
|
Human Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit |
|
abx555435-96tests |
Abbexa |
96 tests |
EUR 786 |
|
|
 ELISA Kit) Mouse Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit |
|
abx555608-96tests |
Abbexa |
96 tests |
EUR 739 |
|
|
 ELISA Kit) Pig Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit |
|
abx555672-96tests |
Abbexa |
96 tests |
EUR 911 |
|
|
 ELISA Kit) Cow Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit |
|
abx555769-96tests |
Abbexa |
96 tests |
EUR 911 |
|
|
 Human TNNC1/ Troponin C, slow skeletal and cardiac muscles ELISA Kit |
|
E2558Hu |
Sunlong |
1 Kit |
EUR 605 |
Human Troponin C, Slow Skeletal And Cardiac Muscles (TNNC1) ELISA Kit |
|
abx352187-96tests |
Abbexa |
96 tests |
EUR 786 |
|
|
 ELISA kit for Human Troponin C, slow skeletal and cardiac muscles |
|
EK3670 |
SAB |
96 tests |
EUR 670 |
|
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Troponin C, slow skeletal and cardiac muscles in samples from serum, plasma, tissue homogenates and other biological fluids. |
) ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) |
|
KTE70102-48T |
Abbkine |
48T |
EUR 332 |
|
|
|
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) |
|
KTE70102-5platesof96wells |
Abbkine |
5 plates of 96 wells |
EUR 2115 |
|
|
|
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) |
|
KTE70102-96T |
Abbkine |
96T |
EUR 539 |
|
|
|
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
) ELISA kit for Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) |
|
KTE50026-48T |
Abbkine |
48T |
EUR 425 |
|
Description: Quantitative sandwich ELISA for measuring Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) |
|
KTE50026-5platesof96wells |
Abbkine |
5 plates of 96 wells |
EUR 3026 |
|
Description: Quantitative sandwich ELISA for measuring Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) |
|
KTE50026-96T |
Abbkine |
96T |
EUR 756 |
|
Description: Quantitative sandwich ELISA for measuring Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
) ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) |
|
KTE30009-48T |
Abbkine |
48T |
EUR 354 |
|
|
|
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) |
|
KTE30009-5platesof96wells |
Abbkine |
5 plates of 96 wells |
EUR 2252 |
|
|
|
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) |
|
KTE30009-96T |
Abbkine |
96T |
EUR 572 |
|
|
|
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
) Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol) |
|
RAT-5 |
Alpha Diagnostics |
1 |
EUR 1138 |
) Total Protein Extraction Kit for Muscles (50 tests) |
|
P5A06 |
101Bio |
- |
Ask for price |
 Rat Skeletal Muscle Tissue Lysate |
|
LYSATE0010 |
BosterBio |
200ug |
EUR 150 |
|
Description: This cell lysate is prepared from rat skeletal muscle tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 rno-miRNome MicroRNA Profiling Kit [Rat] |
|
RA680B-1 |
SBI |
20 complete profiles |
EUR 1672 |
|
|
 MicroRNA Isolation Kit |
|
KS341025 |
Biochain |
1 kit |
EUR 256 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
) Myosin antibody (skeletal) |
|
10R-7875 |
Fitzgerald |
100 ug |
EUR 322 |
|
Description: Mouse monoclonal Myosin antibody |
Myosin antibody (skeletal) |
|
10R-7876 |
Fitzgerald |
100 ug |
EUR 322 |
|
Description: Mouse monoclonal Myosin antibody |
 Skeletal Muscle Lysate |
|
1375 |
ProSci |
0.1 mg |
EUR 191 |
|
Description: Skeletal Muscle tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Skeletal Muscle Lysate |
|
1407 |
ProSci |
0.1 mg |
EUR 191 |
|
Description: Skeletal Muscle tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Skeletal Muscle Lysate |
|
1467 |
ProSci |
0.1 mg |
EUR 191 |
|
Description: Skeletal Muscle tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Skeletal Muscle Lysate |
|
21-107 |
ProSci |
0.1 mg |
EUR 285.5 |
|
Description: Bovine skeletal muscle tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The bovine skeletal muscle tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the skeletal muscle tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The skeletal muscle tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Skeletal Muscle Lysate |
|
21-197 |
ProSci |
0.1 mg |
EUR 285.5 |
|
Description: Monkey (Cynomolgus) skeletal muscle tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) skeletal muscle tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the skeletal muscle tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The skeletal muscle tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
 Cancer MicroRNA qPCR Array |
|
RA610A-1 |
SBI |
10 profiles |
EUR 1172 |
|
|
 Rat Muscle PrimaCell1: Normal Skeletal Muscle Cells |
|
2-82573 |
CHI Scientific |
1 Kit |
Ask for price |
 Protein) Rat Ryanodine Receptor 1, Skeletal (RYR1) Protein |
|
20-abx654972 |
Abbexa |
-
EUR 578.00
-
EUR 258.00
-
EUR 1720.00
-
EUR 690.00
-
EUR 425.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
|
 Troponin-I Rat Skeletal Muscle, pure protein |
|
TNIM50-N-25 |
Alpha Diagnostics |
25 ug |
EUR 225 |
 Rat Skeletal muscle Actinin Alpha ELISA kit |
|
E02S0267-192T |
BlueGene |
192 tests |
EUR 1270 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Skeletal muscle Actinin Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Skeletal muscle Actinin Alpha ELISA kit |
|
E02S0267-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Skeletal muscle Actinin Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Skeletal muscle Actinin Alpha ELISA kit |
|
E02S0267-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Skeletal muscle Actinin Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 cDNA from Rat Normal Tissue: Skeletal Muscle |
|
C1434171 |
Biochain |
40 reactions |
EUR 376 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Troponin I Skeletal Protein |
|
abx060758-100ug |
Abbexa |
100 ug |
EUR 1574 |
|
|
 Troponin T Skeletal Protein |
|
abx060767-05mg |
Abbexa |
0.5 mg |
EUR 1713 |
|
|
 anti-fast skeletal Myosin |
|
YF-PA24194 |
Abfrontier |
50 ul |
EUR 334 |
|
Description: Mouse polyclonal to fast skeletal Myosin |
anti-fast skeletal Myosin |
|
YF-PA13308 |
Abfrontier |
50 ug |
EUR 363 |
|
Description: Mouse polyclonal to fast skeletal Myosin |
 Skeletal Muscle Cytoplasmic Lysate |
|
XBL-10851 |
ProSci |
0.1 mg |
EUR 227.75 |
|
Description: human skeletal muscle tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human skeletal muscle tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated Skeletal Muscle tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated Skeletal Muscle tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot. |
 Skeletal Muscle Membrane Lysate |
|
XBL-10852 |
ProSci |
0.1 mg |
EUR 516.5 |
|
Description: human skeletal muscle tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human skeletal muscle tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated Skeletal Muscle tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated Skeletal Muscle tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
 CLIA Kit) Rat Ryanodine Receptor 1, Skeletal (RYR1) CLIA Kit |
|
20-abx493893 |
Abbexa |
-
EUR 8569.00
-
EUR 4560.00
-
EUR 1052.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
|
 ELISA Kit) Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit |
|
20-abx156055 |
Abbexa |
-
EUR 7237.00
-
EUR 3855.00
-
EUR 895.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
|
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit |
|
DLR-RYR1-Ra-48T |
DL Develop |
48T |
EUR 549 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in samples from tissue homogenates, cell lysates or other biological fluids. |
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit |
|
DLR-RYR1-Ra-96T |
DL Develop |
96T |
EUR 718 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in samples from tissue homogenates, cell lysates or other biological fluids. |
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit |
|
RDR-RYR1-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 583 |
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit |
|
RDR-RYR1-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 811 |
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit |
|
RD-RYR1-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 557 |
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit |
|
RD-RYR1-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 775 |
) Rat Ryanodine Receptor 1, Skeletal ELISA Kit (RYR1) |
|
RK03928 |
Abclonal |
96 Tests |
EUR 521 |
 CLIA Kit) Rat Slow Skeletal Troponin I (TNNI1) CLIA Kit |
|
20-abx494152 |
Abbexa |
-
EUR 8569.00
-
EUR 4560.00
-
EUR 1052.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
|
 CLIA Kit) Rat Fast Skeletal Troponin I (TNNI2) CLIA Kit |
|
20-abx494155 |
Abbexa |
-
EUR 8569.00
-
EUR 4560.00
-
EUR 1052.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
|
 CLIA Kit) Rat Slow Skeletal Troponin T (TNNT1) CLIA Kit |
|
20-abx494158 |
Abbexa |
-
EUR 8569.00
-
EUR 4560.00
-
EUR 1052.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
|
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit |
|
SEC773Ra-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 5124.2 |
|
|
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids. |
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit |
|
SEC773Ra-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 509.64 |
|
|
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids. |
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit |
|
SEC773Ra-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 685.2 |
|
|
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids. |
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit |
|
SEC773Ra-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 2783.4 |
|
|
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids. |
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit |
|
4-SEC773Ra |
Cloud-Clone |
-
EUR 5175.00
-
EUR 2734.00
-
EUR 686.00
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. |
 ELISA kit) Rat Actin, Alpha skeletal muscle(ACTA1) ELISA kit |
|
E02A1218-192T |
BlueGene |
192 tests |
EUR 1270 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Actin, Alpha skeletal muscle(ACTA1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Actin, Alpha skeletal muscle(ACTA1) ELISA kit |
|
E02A1218-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Actin, Alpha skeletal muscle(ACTA1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Actin, Alpha skeletal muscle(ACTA1) ELISA kit |
|
E02A1218-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Actin, Alpha skeletal muscle(ACTA1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 Rat Actin Alpha 1, Skeletal Muscle ELISA kit |
|
E02A0391-192T |
BlueGene |
192 tests |
EUR 1270 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Actin Alpha 1, Skeletal Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Actin Alpha 1, Skeletal Muscle ELISA kit |
|
E02A0391-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Actin Alpha 1, Skeletal Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Actin Alpha 1, Skeletal Muscle ELISA kit |
|
E02A0391-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Actin Alpha 1, Skeletal Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 Rat Muscle, skeletal, receptor tyrosine kinase ELISA kit |
|
E02M0028-192T |
BlueGene |
192 tests |
EUR 1270 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Muscle, skeletal, receptor tyrosine kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Muscle, skeletal, receptor tyrosine kinase ELISA kit |
|
E02M0028-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Muscle, skeletal, receptor tyrosine kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Muscle, skeletal, receptor tyrosine kinase ELISA kit |
|
E02M0028-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Rat Muscle, skeletal, receptor tyrosine kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 Genomic DNA from Rat Normal Tissue: Skeletal Muscle |
|
D1434171 |
Biochain |
100 ug |
EUR 282 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Rat Normal Tissue: Skeletal Muscle |
|
R1434171-50 |
Biochain |
50 ug |
EUR 152 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 SanPrep Column microRNA Miniprep Kit |
|
SK8811 |
Bio Basic |
50prep |
EUR 126.1 |
|
|
 Stem Cell MicroRNA Profiling Panel |
|
RA620A-1 |
SBI |
10 profiles |
EUR 1172 |
|
|
 MicroRNA scramble in CD511 - Virus |
|
PMIRH000VA-1 |
SBI |
1x10^6 IFUs |
EUR 781 |
|
|
 MicroRNA Precursor Pooled Plasmid Library |
|
PMIRHPLPA-1 |
SBI |
1 aliquot plasmid DNA |
EUR 50 |
|
|
 Mouse Skeletal Muscle Tissue Lysate |
|
LYSATE0021 |
BosterBio |
200ug |
EUR 150 |
|
Description: This cell lysate is prepared from mouse skeletal muscle tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 ? skeletal muscle actin Monoclonal Antibody |
|
EM1149-100ul |
ELK Biotech |
100ul |
EUR 279 |
|
Description: A Mouse Monoclonal antibody against ? skeletal muscle actin from Human/ Rat/ Mouse. This antibody is tested and validated for WB, ELISA, IHC |
? skeletal muscle actin Monoclonal Antibody |
|
EM1149-50ul |
ELK Biotech |
50ul |
EUR 207 |
|
Description: A Mouse Monoclonal antibody against ? skeletal muscle actin from Human/ Rat/ Mouse. This antibody is tested and validated for WB, ELISA, IHC |
 ?-Skeletal Muscle Actin Polyclonal Antibody |
|
ES1176-100ul |
ELK Biotech |
100ul |
EUR 279 |
|
Description: A Rabbit Polyclonal antibody against ?-Skeletal Muscle Actin from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA |
?-Skeletal Muscle Actin Polyclonal Antibody |
|
ES1176-50ul |
ELK Biotech |
50ul |
EUR 207 |
|
Description: A Rabbit Polyclonal antibody against ?-Skeletal Muscle Actin from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA |
?-Skeletal Muscle Actin Polyclonal Antibody |
|
EA047-100ul |
ELK Biotech |
100ul |
EUR 279 |
|
Description: A Rabbit Polyclonal antibody against ?-Skeletal Muscle Actin from Human/ Rat/ Mouse. This antibody is tested and validated for WB, ELISA, IHC |
?-Skeletal Muscle Actin Polyclonal Antibody |
|
EA047-50ul |
ELK Biotech |
50ul |
EUR 207 |
|
Description: A Rabbit Polyclonal antibody against ?-Skeletal Muscle Actin from Human/ Rat/ Mouse. This antibody is tested and validated for WB, ELISA, IHC |
 Troponin I Skeletal Muscle Antibody |
|
abx020870-1mg |
Abbexa |
1 mg |
EUR 815 |
|
|
Troponin I Skeletal Muscle Antibody |
|
abx020871-1mg |
Abbexa |
1 mg |
EUR 815 |
|
|
 Rabbit Skeletal Muscle Troponin-C |
|
7-06205 |
CHI Scientific |
1mg |
Ask for price |
Rabbit Skeletal Muscle Troponin-C |
|
7-06206 |
CHI Scientific |
5mg |
Ask for price |
Rabbit Skeletal Muscle Troponin-C |
|
7-06207 |
CHI Scientific |
10mg |
Ask for price |
The findings unveil that siRNAs focused to five’ UTR-IRES of HCV sub-genotype 4a Saudi isolates present potent silencing of HCV replication and blocking of viral translation in a persistent in-vitro Huh-7 tissue tradition system. Moreover, we additionally elucidated that siRNA silencing of viral mRNA not solely inhibits viral replication but in addition blocks viral translation. The outcomes recommend that siRNA potent antiviral exercise needs to be thought-about as an efficient anti-mRNA based mostly remedy methods for additional in-vivo investigations towards much less studied and harder-to-treat HCV sub-genotype 4a isolates in Saudi Arabia.
