Throughout transcription initiation, the final transcription issue TFIIH marks RNA polymerase II by phosphorylating Ser5 of the carboxyl-terminal area (CTD) of Rpb1, which is adopted by in depth modifications coupled to transcription elongation, mRNA processing, and histone dynamics. We now have decided a 3.5-Å decision cryo-electron microscopy (cryo-EM) construction of the TFIIH kinase module (TFIIK in yeast), which consists of Kin28, Ccl1, and Tfb3, yeast homologs of CDK7, cyclin H, and MAT1, respectively. The carboxyl-terminal area of Tfb3 was mendacity on the fringe of catalytic cleft of Kin28, the place a conserved Tfb3 helix served to stabilize the activation loop in its lively conformation. By combining the construction of TFIIK with the earlier cryo-EM construction of the preinitiation advanced, we prolong the beforehand proposed mannequin of the CTD path to the lively web site of TFIIK.
The construction of Prp2 sure to RNA and ADP-BeF 3– reveals structural options necessary for RNA unwinding by DEAH-box ATPases
Noncoding intron sequences current in precursor mRNAs have to be eliminated previous to translation, and they’re excised by way of the spliceosome, a multimegadalton molecular machine composed of quite a few protein and RNA parts. The DEAH-box ATPase Prp2 performs an important function throughout pre-mRNA splicing because it ensures the catalytic activation of the spliceosome. Regardless of excessive structural similarity to different spliceosomal DEAH-box helicases, Prp2 doesn’t appear to operate as an RNA helicase, however fairly as an RNA-dependent ribonucleoprotein particle-modifying ATPase. Latest crystal buildings of the spliceosomal DEAH-box ATPases Prp43 and Prp22, in addition to of the associated RNA helicase MLE, in advanced with RNA have contributed to a greater understanding of how RNA binding and processivity may be achieved on this helicase household.
As a way to shed mild onto the divergent method of operate of Prp2, an N-terminally truncated assemble of Chaetomium thermophilum Prp2 was crystallized within the presence of ADP-BeF3– and a poly-U12 RNA. The refined construction revealed a just about equivalent conformation of the helicase core in contrast with the ADP-BeF3–– and RNA-bound construction of Prp43, and solely a minor shift of the C-terminal domains. Nonetheless, Prp2 and Prp43 differ within the hook-loop and a loop of the helix-bundle area, which interacts with the hook-loop and evokes a unique RNA conformation instantly after the three’ stack. On changing these loop residues in Prp43 by the Prp2 sequence, the unwinding exercise of Prp43 was abolished. Moreover, a putative exit tunnel for the γ-phosphate after ATP hydrolysis could possibly be recognized in one of many Prp2 buildings.
Combinatorial optimization of mRNA construction, stability, and translation for RNA-based therapeutics
Therapeutic mRNAs and vaccines are being developed for a broad vary of human illnesses, together with COVID-19. Nonetheless, their optimization is hindered by mRNA instability and inefficient protein expression. Right here, we describe design ideas that overcome these obstacles. We develop a brand new RNA sequencing-based platform known as PERSIST-seq to systematically delineate in-cell mRNA stability, ribosome load, in addition to in-solution stability of a library of various mRNAs.
We discover that, surprisingly, in-cell stability is a higher driver of protein output than excessive ribosome load. We additional introduce a technique known as In-line-seq, utilized to hundreds of various RNAs, that reveals sequence and structure-based guidelines for mitigating hydrolytic degradation. Our findings present that “superfolder” mRNAs might be designed to enhance each stability and expression which are additional enhanced by pseudouridine nucleoside modification. Collectively, our research demonstrates simultaneous enchancment of mRNA stability and protein expression and offers a computational-experimental platform for the enhancement of mRNA medicines.
Small molecule focusing on of biologically related RNA tertiary and quaternary buildings
Preliminary successes in creating small molecule ligands for non-coding RNAs have underscored their potential as therapeutic targets. Extra lately, these successes have been aided by advances in biophysical and structural methods for identification and characterization of extra advanced RNA buildings; these higher-level folds current protein-like binding pockets that provide alternatives to design small molecules that might obtain a level of selectivity typically onerous to acquire on the main and secondary construction degree. Extra particularly, identification and small molecule focusing on of RNA tertiary and quaternary buildings have allowed researchers to probe a number of human illnesses and have resulted in promising scientific candidates.
On this evaluation we spotlight a number of various and thrilling successes and the experimental approaches that led to their discovery. These research embody examples of latest developments in RNA-centric assays and ligands that present perception into the options liable for the affinity and organic consequence of RNA-targeted chemical probes. This report highlights the potential and rising alternatives to selectively goal RNA tertiary and quaternary buildings as a route to raised perceive and, in the end, deal with many illnesses.

RNA sequence and construction determinants of Pol III transcriptional termination in human cells
The exact mechanism of transcription termination of the eukaryotic RNA polymerase III (Pol III) has been a topic of appreciable debate. Though earlier research have clearly proven that a number of uracils on the finish of RNA transcripts are required for Pol III termination, the consequences of upstream RNA secondary construction within the nascent transcript on transcriptional termination remains to be unclear. To handle this, we developed an in cellulo Pol III transcription termination assay utilizing the lately developed Twister-Corn RNA aptamer system to create a Pol III-transcribed RNA that produces a detectable fluorescent sign when transcribed in human cells.
To review the consequences of RNA sequence and construction on Pol III termination, we systematically different the sequence context upstream of the aptamer and recognized sequence traits that improve or diminish termination. For transcription from Pol III sort Three promoters, we discovered that solely poly-U tracts longer than the common size discovered within the human genome effectively terminate Pol III transcription with out RNA secondary construction parts. We noticed that RNA secondary construction parts positioned in proximity to shorter poly-U tracts induced termination, and RNA secondary construction by itself was not ample to induce termination.
Monkey Cancer/testis antigen 47B(CT47B1) ELISA kit |
E09C2139-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen 47B(CT47B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen 47B(CT47B1) ELISA kit |
E09C2139-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen 47B(CT47B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen 1(CTAG1A) ELISA kit |
E09C2140-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 1(CTAG1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen 1(CTAG1A) ELISA kit |
E09C2140-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 1(CTAG1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen 1(CTAG1A) ELISA kit |
E09C2140-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 1(CTAG1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen 2(CTAG2) ELISA kit |
E09C2141-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 2(CTAG2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen 2(CTAG2) ELISA kit |
E09C2141-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 2(CTAG2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen 2(CTAG2) ELISA kit |
E09C2141-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 2(CTAG2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Bromodomain testis specific protein(BRDT) ELISA kit |
E09B0840-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Bromodomain testis specific protein(BRDT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Bromodomain testis specific protein(BRDT) ELISA kit |
E09B0840-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Bromodomain testis specific protein(BRDT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Bromodomain testis specific protein(BRDT) ELISA kit |
E09B0840-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Bromodomain testis specific protein(BRDT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Testis tumor with testis tissue array |
BC23011a |
TissueArray |
each |
EUR 270 |
Description: Testis tumor with testis tissue array, including TNM and clinical stage (reference AJCC 8th version), 24 cases/72 cores, replacing BC23011 |
Testis cancer with testis tissue array |
TE481a |
TissueArray |
each |
EUR 198 |
Description: Testis cancer with testis tissue array, including pathology grade, TNM and cinical stage, 48 cases/48 cores, replacing TE481 |
Testis Lysate |
1416 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Testis tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Mouse ; Testis |
02-715 |
Sceti |
100ng |
EUR 481.2 |
Description: The Mouse ; Testis is available in Europe and for worldwide shipping via Gentaur. |
Testis Lysate |
1476 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Testis tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Monkey Testis expressed sequence 10 protein(TEX10) ELISA kit |
E09T0680-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Testis expressed sequence 10 protein(TEX10) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Testis expressed sequence 10 protein(TEX10) ELISA kit |
E09T0680-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Testis expressed sequence 10 protein(TEX10) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Testis expressed sequence 10 protein(TEX10) ELISA kit |
E09T0680-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Testis expressed sequence 10 protein(TEX10) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Multiple testis tumor and testis tissue array |
TE482a |
TissueArray |
each |
EUR 168 |
Description: Multiple testis tumor and testis tissue array, including TNM and clinical stage, 24 cases/48 cores, replacing TE482 |
Cynomolgus Monkey: Testis (single section per slide) (5 slides/pack) |
CyFPT181 |
TissueArray |
5 slides |
EUR 150 |
Testis tumor with adjacent normal testis tissue array |
TE242 |
TissueArray |
each |
EUR 66 |
Description: Testis tumor with adjacent normal testis tissue array, including TNM and clinical stage, 12 cases/24 cores, replacing TE241 |
Testis Membrane Lysate |
XBL-10957 |
ProSci |
0.1 mg |
EUR 619.8 |
Description: Human testis tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human testis tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated testis tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated testis tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
MicroRNA Isolation Kit |
KS341025 |
Biochain |
1 kit |
EUR 307.2 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
Monkey Fetal and adult testis expressed transcript protein(FATE1) ELISA kit |
E09F0361-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Fetal and adult testis expressed transcript protein(FATE1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Fetal and adult testis expressed transcript protein(FATE1) ELISA kit |
E09F0361-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Fetal and adult testis expressed transcript protein(FATE1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Fetal and adult testis expressed transcript protein(FATE1) ELISA kit |
E09F0361-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Fetal and adult testis expressed transcript protein(FATE1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A1(CT45A1) ELISA kit |
E09C2133-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A1(CT45A1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A1(CT45A1) ELISA kit |
E09C2133-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A1(CT45A1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A1(CT45A1) ELISA kit |
E09C2133-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A1(CT45A1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A2(CT45A2) ELISA kit |
E09C2134-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A2(CT45A2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A2(CT45A2) ELISA kit |
E09C2134-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A2(CT45A2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A2(CT45A2) ELISA kit |
E09C2134-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A2(CT45A2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A3(CT45A3) ELISA kit |
E09C2135-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A3(CT45A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A3(CT45A3) ELISA kit |
E09C2135-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A3(CT45A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A3(CT45A3) ELISA kit |
E09C2135-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A3(CT45A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A5(CT45A5) ELISA kit |
E09C2137-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A5(CT45A5) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A5(CT45A5) ELISA kit |
E09C2137-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A5(CT45A5) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A5(CT45A5) ELISA kit |
E09C2137-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A5(CT45A5) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Testis Tissue Slide (Normal) |
11-701-10um |
ProSci |
10 um |
EUR 241.8 |
Testis Tissue Slide (Normal) |
11-701-4um |
ProSci |
4 um |
EUR 216.6 |
Testis Tissue Slide (Abnormal) |
11-710-10um |
ProSci |
10 um |
EUR 241.8 |
Testis Tissue Slide (Abnormal) |
11-710-4um |
ProSci |
4 um |
EUR 216.6 |
Rat Testis Tissue Lysate |
LYSATE0005 |
BosterBio |
200ug |
EUR 180 |
Description: This cell lysate is prepared from rat testis tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
Mouse Testis Tissue Lysate |
LYSATE0014 |
BosterBio |
200ug |
EUR 180 |
Description: This cell lysate is prepared from mouse testis tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
Testis Liver Cirrhosis Lysate |
XBL-10389 |
ProSci |
0.1 mg |
EUR 796.2 |
Description: Human testis tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human testis tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the testis tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The testis tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Testis Membrane Tumor Lysate |
XBL-10966 |
ProSci |
0.1 mg |
EUR 752.1 |
Description: Human testis tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human testis tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated testis tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated testis tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
Mouse Testis Nuclear Extract |
X12009 |
EpiGentek |
500 µg |
Ask for price |
Testis tumor tissue array |
BC23012 |
TissueArray |
each |
EUR 270 |
Description: Testis tumor tissue array, 23 cases/63 cores |
Testis spectrum tissue array |
TE810 |
TissueArray |
each |
EUR 306 |
Description: Testis spectrum tissue array, including TNM and clinical stage (AJCC 8.0), 81 cases/81 cores (1.5mm), replacingTE803 |
Normal testis tissue array |
TEN241 |
TissueArray |
each |
EUR 198 |
Description: Normal testis tissue array, 24 cases/24 cores |
Normal testis tissue array |
TEN601 |
TissueArray |
each |
EUR 222 |
Description: Normal testis tissue array, 30 cases/ 60 cores |
Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) ELISA kit |
E09C2136-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) ELISA kit |
E09C2136-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) ELISA kit |
E09C2136-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Cynomolgus Monkey: Testis(deferent duct) (single section per slide) (5 slides/pack) |
CyFPT182 |
TissueArray |
5 slides |
EUR 150 |
Testis Tumor Tissue Array - 16 cases of testis tumors and 8 cases of non-tumor testis tissues. |
Z7020096 |
Biochain |
5 slides |
EUR 1657.2 |
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
Cancer MicroRNA qPCR Array |
RA610A-1 |
SBI |
10 profiles |
EUR 1406.4 |
|
Recombinant Human Testis Derived Transcript |
7-06631 |
CHI Scientific |
2µg |
Ask for price |
Recombinant Human Testis Derived Transcript |
7-06632 |
CHI Scientific |
10µg |
Ask for price |
Recombinant Human Testis Derived Transcript |
7-06633 |
CHI Scientific |
1mg |
Ask for price |
Testis Specific 10 (TSGA10) Antibody |
abx145551-100ug |
Abbexa |
100 ug |
EUR 469.2 |
|
Testis Specific 10 (TSGA10) Antibody |
20-abx003819 |
Abbexa |
|
|
|
Testis Expressed 10 (TEX10) Antibody |
20-abx116034 |
Abbexa |
|
|
|
Testis Expressed 11 (TEX11) Antibody |
20-abx116035 |
Abbexa |
|
|
|
Testis Expressed 12 (TEX12) Antibody |
20-abx116036 |
Abbexa |
|
|
|
Testis Expressed 13A (TEX13A) Antibody |
20-abx116037 |
Abbexa |
|
|
|
Testis Expressed 14 (TEX14) Antibody |
20-abx116038 |
Abbexa |
|
|
|
Testis Specific, 10 (TSGA10) Antibody |
20-abx116039 |
Abbexa |
|
|
|
Testis Expressed 12 (TEX12) Antibody |
abx030409-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Testis Expressed 12 (TEX12) Antibody |
abx030409-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Testis Specific 10 (TSGA10) Antibody |
abx031103-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Testis Specific 10 (TSGA10) Antibody |
abx031103-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Testis Specific 13 (TSGA13) Antibody |
20-abx302813 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Testis Specific 10 (TSGA10) Antibody |
20-abx302841 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Testis Expressed 13A (TEX13A) Antibody |
20-abx311757 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Testis Expressed 11 (TEX11) Antibody |
abx433354-200ul |
Abbexa |
200 ul |
EUR 343.2 |
|
Testis Expressed 10 (TEX10) Antibody |
abx238604-100ug |
Abbexa |
100 ug |
EUR 610.8 |
|
Testis Expressed 101 (TEX101) Antibody |
abx238605-100ug |
Abbexa |
100 ug |
EUR 661.2 |
|
Testis Expressed 11 (TEX11) Antibody |
abx238606-100ug |
Abbexa |
100 ug |
EUR 610.8 |
|
Testis Expressed 12 (TEX12) Antibody |
abx238607-100ug |
Abbexa |
100 ug |
EUR 610.8 |
|
Testis Expressed 13A (TEX13A) Antibody |
abx238608-100ug |
Abbexa |
100 ug |
EUR 661.2 |
|
Testis Expressed 264 (TEX264) Antibody |
abx238611-100ug |
Abbexa |
100 ug |
EUR 610.8 |
|
Testis Expressed 38 (TEX38) Antibody |
abx238613-100ug |
Abbexa |
100 ug |
EUR 610.8 |
|
Testis Specific 10 (TSGA10) Antibody |
abx239042-100ug |
Abbexa |
100 ug |
EUR 610.8 |
|
Testis Specific 13 (TSGA13) Antibody |
abx239043-100ug |
Abbexa |
100 ug |
EUR 661.2 |
|
Testis cancer test tissue array |
T233 |
TissueArray |
each |
EUR 66 |
Description: Testis cancer test tissue array, with normal tissue as control, including TNM and clinical stage, 2 serial sections, 6 cases/24 cores |
MicroRNA scramble in CD511 - Virus |
PMIRH000VA-1 |
SBI |
1x10^6 IFUs |
EUR 937.2 |
|
MicroRNA Precursor Pooled Plasmid Library |
PMIRHPLPA-1 |
SBI |
1 aliquot plasmid DNA |
EUR 60 |
|
SanPrep Column microRNA Miniprep Kit |
SK8811 |
Bio Basic |
50prep |
EUR 151.32 |
|
Stem Cell MicroRNA Profiling Panel |
RA620A-1 |
SBI |
10 profiles |
EUR 1406.4 |
|
Multiple Species Tissue Slides - Testis Tissue |
11-700-MSTA |
ProSci |
1 pack |
EUR 241.8 |
Description: The Multiple Species Tissue Array (MSTA) slides were designed to study protein expression patterns in different cells and tissues from multiple species. Tissue slices from three different species are mounted on each MSTA slide which can then be treated as a single histological slide for H&E staining, immunohistochemistry, or in situ hybridization. This format allows a rapid analysis of protein expression and localization across different species. MSTA slides can also be used to quickly determine the species reactivity of a given antibody. |
Human Testis OptiTDS: Tissue Dissociation System |
4-28105 |
CHI Scientific |
1 Kit |
Ask for price |
Human Testis PrimaCell: Normal Sertoli Cells |
2-96105 |
CHI Scientific |
1 Kit |
Ask for price |
Cancer/testis Antigen 2 (CTAG2) Antibody |
20-abx149592 |
Abbexa |
|
|
|
Cancer/Testis Antigen 1B (CTAG1B) Antibody |
20-abx171540 |
Abbexa |
|
|
|
Cancer/Testis Antigen 1B (CTAG1B) Antibody |
20-abx135754 |
Abbexa |
-
EUR 493.20
-
EUR 710.40
-
EUR 218.40
-
EUR 376.80
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
Cancer/Testis Antigen 5.1 (SSX1) Antibody |
abx145140-100ug |
Abbexa |
100 ug |
EUR 469.2 |
|
Testis Specific Kinase 1 (TESK1) Antibody |
20-abx008652 |
Abbexa |
-
EUR 360.00
-
EUR 526.80
-
EUR 226.80
|
|
|
Testis-expressed sequence 12 protein Antibody |
20-abx110259 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Testis Specific Kinase 1 (TESK1) Antibody |
20-abx130977 |
Abbexa |
-
EUR 526.80
-
EUR 159.60
-
EUR 1479.60
-
EUR 710.40
-
EUR 393.60
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Testis Specific Kinase 1 (TESK1) Antibody |
20-abx130978 |
Abbexa |
-
EUR 510.00
-
EUR 159.60
-
EUR 1446.00
-
EUR 693.60
-
EUR 393.60
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Cancer/Testis Antigen 1A (CTAG1A) Antibody |
20-abx126874 |
Abbexa |
|
|
|
Cancer/Testis Antigen 1B (CTAG1B) Antibody |
20-abx128209 |
Abbexa |
-
EUR 510.00
-
EUR 159.60
-
EUR 1446.00
-
EUR 693.60
-
EUR 393.60
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Cancer/Testis Antigen 1A (CTAG1A) Antibody |
20-abx111352 |
Abbexa |
|
|
|
Testis Anion Transporter 1 (TAT1) Antibody |
20-abx129200 |
Abbexa |
-
EUR 510.00
-
EUR 159.60
-
EUR 1446.00
-
EUR 693.60
-
EUR 393.60
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Phosphorylase Kinase, gamma 2 (Testis) Antibody |
20-abx114507 |
Abbexa |
|
|
|
Testis Serine Protease 1 (TESSP1) Antibody |
abx026869-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Testis Serine Protease 1 (TESSP1) Antibody |
abx026869-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Cancer/Testis Antigen 1A (CTAG1A) Antibody |
abx027441-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Cancer/Testis Antigen 1A (CTAG1A) Antibody |
abx027441-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Cancer/testis Antigen 2 (CTAG2) Antibody |
abx027593-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Cancer/testis Antigen 2 (CTAG2) Antibody |
abx027593-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Testis-Expressed Protein 33 (EAN57) Antibody |
abx029059-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Testis-Expressed Protein 33 (EAN57) Antibody |
abx029059-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Testis Specific Kinase 1 (TESK1) Antibody |
abx029069-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Testis Specific Kinase 1 (TESK1) Antibody |
abx029069-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Testis-Expressed Protein 2 (TEX2) Antibody |
abx029879-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Testis-Expressed Protein 2 (TEX2) Antibody |
abx029879-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Testis-Expressed Protein 9 (TEX9) Antibody |
abx025800-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Testis-Expressed Protein 9 (TEX9) Antibody |
abx025800-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Testis-Expressed Protein 13B (TEX13B) Antibody |
abx025974-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Testis-Expressed Protein 13B (TEX13B) Antibody |
abx025974-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Bromodomain Testis-Specific Protein (BRDT) Antibody |
abx025117-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Bromodomain Testis-Specific Protein (BRDT) Antibody |
abx025117-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Testis Specific Kinase 1 (TESK1) Antibody |
abx331203-100ul |
Abbexa |
100 ul |
EUR 510 |
|
Cancer/Testis Antigen 1A (CTAG1A) Antibody |
abx331930-100ul |
Abbexa |
100 ul |
EUR 510 |
|
Testis-Expressed Protein 43 (TEX43) Antibody |
20-abx301458 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Cancer/Testis Antigen 5.1 (SSX1) Antibody |
20-abx302290 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Testis-Expressed Protein 35 (Tex35) Antibody |
20-abx307697 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Cancer/Testis Antigen 5.1 (SSX1) Antibody |
20-abx323587 |
Abbexa |
|
|
|
Testis Expressed 13A (TEX13A) Antibody (HRP) |
20-abx311758 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
For Pol III sort 2 promoters, we discovered that the shorter poly-U tract lengths of four uracils had been ample to induce termination. These findings reveal a key function for sequence and structural parts inside Pol III-transcribed nascent RNA for environment friendly transcription termination, and reveal a generalizable assay for characterizing Pol III transcription in human cells.