Throughout transcription initiation, the final transcription issue TFIIH marks RNA polymerase II by phosphorylating Ser5 of the carboxyl-terminal area (CTD) of Rpb1, which is adopted by in depth modifications coupled to transcription elongation, mRNA processing, and histone dynamics. We now have decided a 3.5-Å decision cryo-electron microscopy (cryo-EM) construction of the TFIIH kinase module (TFIIK in yeast), which consists of Kin28, Ccl1, and Tfb3, yeast homologs of CDK7, cyclin H, and MAT1, respectively. The carboxyl-terminal area of Tfb3 was mendacity on the fringe of catalytic cleft of Kin28, the place a conserved Tfb3 helix served to stabilize the activation loop in its lively conformation. By combining the construction of TFIIK with the earlier cryo-EM construction of the preinitiation advanced, we prolong the beforehand proposed mannequin of the CTD path to the lively web site of TFIIK.
The construction of Prp2 sure to RNA and ADP-BeF 3– reveals structural options necessary for RNA unwinding by DEAH-box ATPases
Noncoding intron sequences current in precursor mRNAs have to be eliminated previous to translation, and they’re excised by way of the spliceosome, a multimegadalton molecular machine composed of quite a few protein and RNA parts. The DEAH-box ATPase Prp2 performs an important function throughout pre-mRNA splicing because it ensures the catalytic activation of the spliceosome. Regardless of excessive structural similarity to different spliceosomal DEAH-box helicases, Prp2 doesn’t appear to operate as an RNA helicase, however fairly as an RNA-dependent ribonucleoprotein particle-modifying ATPase. Latest crystal buildings of the spliceosomal DEAH-box ATPases Prp43 and Prp22, in addition to of the associated RNA helicase MLE, in advanced with RNA have contributed to a greater understanding of how RNA binding and processivity may be achieved on this helicase household.
As a way to shed mild onto the divergent method of operate of Prp2, an N-terminally truncated assemble of Chaetomium thermophilum Prp2 was crystallized within the presence of ADP-BeF3– and a poly-U12 RNA. The refined construction revealed a just about equivalent conformation of the helicase core in contrast with the ADP-BeF3–– and RNA-bound construction of Prp43, and solely a minor shift of the C-terminal domains. Nonetheless, Prp2 and Prp43 differ within the hook-loop and a loop of the helix-bundle area, which interacts with the hook-loop and evokes a unique RNA conformation instantly after the three’ stack. On changing these loop residues in Prp43 by the Prp2 sequence, the unwinding exercise of Prp43 was abolished. Moreover, a putative exit tunnel for the γ-phosphate after ATP hydrolysis could possibly be recognized in one of many Prp2 buildings.
Combinatorial optimization of mRNA construction, stability, and translation for RNA-based therapeutics
Therapeutic mRNAs and vaccines are being developed for a broad vary of human illnesses, together with COVID-19. Nonetheless, their optimization is hindered by mRNA instability and inefficient protein expression. Right here, we describe design ideas that overcome these obstacles. We develop a brand new RNA sequencing-based platform known as PERSIST-seq to systematically delineate in-cell mRNA stability, ribosome load, in addition to in-solution stability of a library of various mRNAs.
We discover that, surprisingly, in-cell stability is a higher driver of protein output than excessive ribosome load. We additional introduce a technique known as In-line-seq, utilized to hundreds of various RNAs, that reveals sequence and structure-based guidelines for mitigating hydrolytic degradation. Our findings present that “superfolder” mRNAs might be designed to enhance each stability and expression which are additional enhanced by pseudouridine nucleoside modification. Collectively, our research demonstrates simultaneous enchancment of mRNA stability and protein expression and offers a computational-experimental platform for the enhancement of mRNA medicines.
Small molecule focusing on of biologically related RNA tertiary and quaternary buildings
Preliminary successes in creating small molecule ligands for non-coding RNAs have underscored their potential as therapeutic targets. Extra lately, these successes have been aided by advances in biophysical and structural methods for identification and characterization of extra advanced RNA buildings; these higher-level folds current protein-like binding pockets that provide alternatives to design small molecules that might obtain a level of selectivity typically onerous to acquire on the main and secondary construction degree. Extra particularly, identification and small molecule focusing on of RNA tertiary and quaternary buildings have allowed researchers to probe a number of human illnesses and have resulted in promising scientific candidates.
On this evaluation we spotlight a number of various and thrilling successes and the experimental approaches that led to their discovery. These research embody examples of latest developments in RNA-centric assays and ligands that present perception into the options liable for the affinity and organic consequence of RNA-targeted chemical probes. This report highlights the potential and rising alternatives to selectively goal RNA tertiary and quaternary buildings as a route to raised perceive and, in the end, deal with many illnesses.
RNA sequence and construction determinants of Pol III transcriptional termination in human cells
The exact mechanism of transcription termination of the eukaryotic RNA polymerase III (Pol III) has been a topic of appreciable debate. Though earlier research have clearly proven that a number of uracils on the finish of RNA transcripts are required for Pol III termination, the consequences of upstream RNA secondary construction within the nascent transcript on transcriptional termination remains to be unclear. To handle this, we developed an in cellulo Pol III transcription termination assay utilizing the lately developed Twister-Corn RNA aptamer system to create a Pol III-transcribed RNA that produces a detectable fluorescent sign when transcribed in human cells.
To review the consequences of RNA sequence and construction on Pol III termination, we systematically different the sequence context upstream of the aptamer and recognized sequence traits that improve or diminish termination. For transcription from Pol III sort Three promoters, we discovered that solely poly-U tracts longer than the common size discovered within the human genome effectively terminate Pol III transcription with out RNA secondary construction parts. We noticed that RNA secondary construction parts positioned in proximity to shorter poly-U tracts induced termination, and RNA secondary construction by itself was not ample to induce termination.
 ELISA kit) Monkey Cancer/testis antigen 1(CTAG1A) ELISA kit |
|
E09C2140-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 1(CTAG1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen 1(CTAG1A) ELISA kit |
|
E09C2140-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 1(CTAG1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 ELISA kit) Monkey Cancer/testis antigen 47A(CT47A1) ELISA kit |
|
E09C2138-192T |
BlueGene |
192 tests |
EUR 1524 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen 47A(CT47A1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen 47A(CT47A1) ELISA kit |
|
E09C2138-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen 47A(CT47A1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen 47A(CT47A1) ELISA kit |
|
E09C2138-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen 47A(CT47A1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 ELISA kit) Monkey Cancer/testis antigen 47B(CT47B1) ELISA kit |
|
E09C2139-192T |
BlueGene |
192 tests |
EUR 1524 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen 47B(CT47B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen 47B(CT47B1) ELISA kit |
|
E09C2139-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen 47B(CT47B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen 47B(CT47B1) ELISA kit |
|
E09C2139-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen 47B(CT47B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 MicroRNA Isolation Kit |
|
KS341025 |
Biochain |
1 kit |
EUR 247 |
 ELISA kit) Monkey Testis expressed sequence 10 protein(TEX10) ELISA kit |
|
E09T0680-192T |
BlueGene |
192 tests |
EUR 1524 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Testis expressed sequence 10 protein(TEX10) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Testis expressed sequence 10 protein(TEX10) ELISA kit |
|
E09T0680-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Testis expressed sequence 10 protein(TEX10) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Testis expressed sequence 10 protein(TEX10) ELISA kit |
|
E09T0680-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Testis expressed sequence 10 protein(TEX10) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 Cancer MicroRNA qPCR Array |
|
RA610A-1 |
SBI |
10 profiles |
EUR 1406.4 |
|
|
 ELISA kit) Monkey Bromodomain testis specific protein(BRDT) ELISA kit |
|
E09B0840-192T |
BlueGene |
192 tests |
EUR 1524 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Bromodomain testis specific protein(BRDT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Bromodomain testis specific protein(BRDT) ELISA kit |
|
E09B0840-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Bromodomain testis specific protein(BRDT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Bromodomain testis specific protein(BRDT) ELISA kit |
|
E09B0840-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Bromodomain testis specific protein(BRDT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 (5 slides/pack)) Cynomolgus Monkey: Testis (single section per slide) (5 slides/pack) |
|
CyFPT181 |
TissueArray |
5 slides |
EUR 150 |
 MicroRNA scramble in CD511 - Virus |
|
PMIRH000VA-1 |
SBI |
1x10^6 IFUs |
EUR 715 |
 Stem Cell MicroRNA Profiling Panel |
|
RA620A-1 |
SBI |
10 profiles |
EUR 1406.4 |
|
|
 SanPrep Column microRNA Miniprep Kit |
|
SK8811 |
Bio Basic |
50prep |
EUR 151.32 |
|
|
 ELISA kit) Monkey Cancer/testis antigen family 45 member A1(CT45A1) ELISA kit |
|
E09C2133-192T |
BlueGene |
192 tests |
EUR 1524 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A1(CT45A1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A1(CT45A1) ELISA kit |
|
E09C2133-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A1(CT45A1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A1(CT45A1) ELISA kit |
|
E09C2133-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A1(CT45A1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 ELISA kit) Monkey Cancer/testis antigen family 45 member A2(CT45A2) ELISA kit |
|
E09C2134-192T |
BlueGene |
192 tests |
EUR 1524 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A2(CT45A2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A2(CT45A2) ELISA kit |
|
E09C2134-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A2(CT45A2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A2(CT45A2) ELISA kit |
|
E09C2134-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A2(CT45A2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 ELISA kit) Monkey Cancer/testis antigen family 45 member A3(CT45A3) ELISA kit |
|
E09C2135-192T |
BlueGene |
192 tests |
EUR 1524 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A3(CT45A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A3(CT45A3) ELISA kit |
|
E09C2135-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A3(CT45A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A3(CT45A3) ELISA kit |
|
E09C2135-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A3(CT45A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 ELISA kit) Monkey Cancer/testis antigen family 45 member A5(CT45A5) ELISA kit |
|
E09C2137-192T |
BlueGene |
192 tests |
EUR 1524 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A5(CT45A5) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A5(CT45A5) ELISA kit |
|
E09C2137-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A5(CT45A5) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A5(CT45A5) ELISA kit |
|
E09C2137-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A5(CT45A5) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 (single section per slide) (5 slides/pack)) Cynomolgus Monkey: Testis(deferent duct) (single section per slide) (5 slides/pack) |
|
CyFPT182 |
TissueArray |
5 slides |
EUR 150 |
 ELISA kit) Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) ELISA kit |
|
E09C2136-192T |
BlueGene |
192 tests |
EUR 1524 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) ELISA kit |
|
E09C2136-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) ELISA kit |
|
E09C2136-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 MicroRNA Precursor Pooled Plasmid Library |
|
PMIRHPLPA-1 |
SBI |
1 aliquot plasmid DNA |
EUR 60 |
|
|
 rno-miRNome MicroRNA Profiling Kit [Rat] |
|
RA680B-1 |
SBI |
20 complete profiles |
EUR 2006.4 |
|
|
 hsa-miRNome MicroRNA Profiling Kit [Human] |
|
RA660A-1 |
SBI |
20 profiles |
EUR 2212.8 |
|
|
 mmu-miRNome MicroRNA Profiling Kit [Mouse] |
|
RA670A-1 |
SBI |
20 profiles |
EUR 2046 |
|
|
 ELISA kit) Monkey Fetal and adult testis expressed transcript protein(FATE1) ELISA kit |
|
E09F0361-192T |
BlueGene |
192 tests |
EUR 1524 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Fetal and adult testis expressed transcript protein(FATE1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Fetal and adult testis expressed transcript protein(FATE1) ELISA kit |
|
E09F0361-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Fetal and adult testis expressed transcript protein(FATE1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Fetal and adult testis expressed transcript protein(FATE1) ELISA kit |
|
E09F0361-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Monkey Fetal and adult testis expressed transcript protein(FATE1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 miRZip-1 anti-miR-1 microRNA construct |
|
MZIP1-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-7 anti-miR-7 microRNA construct |
|
MZIP7-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-9 anti-miR-9 microRNA construct |
|
MZIP9-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 OncoMir pooled microRNA precursor virus library |
|
PMIRHOPLVA-1 |
SBI |
1 virus aliquot |
EUR 1429.2 |
|
|
 miRZip-16 anti-miR-16 microRNA construct |
|
MZIP16-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-17 anti-miR-17 microRNA construct |
|
MZIP17-AA-1 |
SBI |
Miniprep DNA |
EUR 620 |
miRZip-17 anti-miR-17 microRNA construct |
|
MZIP17-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-21 anti-miR-21 microRNA construct |
|
MZIP21-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-22 anti-miR-22 microRNA construct |
|
MZIP22-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-24 anti-miR-24 microRNA construct |
|
MZIP24-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-25 anti-miR-25 microRNA construct |
|
MZIP25-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-31 anti-miR-31 microRNA construct |
|
MZIP31-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-32 anti-miR-32 microRNA construct |
|
MZIP32-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-93 anti-miR-93 microRNA construct |
|
MZIP93-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-95 anti-miR-95 microRNA construct |
|
MZIP95-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-96 anti-miR-96 microRNA construct |
|
MZIP96-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-98 anti-miR-98 microRNA construct |
|
MZIP98-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 MicroRNA precursor pooled library, HT prepackaged |
|
PMIRHPLVAHT-1 |
SBI |
10 virus aliquots |
EUR 9763 |
 miRZip-100 anti-miR-100 microRNA construct |
|
MZIP100-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-101 anti-miR-101 microRNA construct |
|
MZIP101-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-103 anti-miR-103 microRNA construct |
|
MZIP103-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-107 anti-miR-107 microRNA construct |
|
MZIP107-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-10a anti-miR-10a microRNA construct |
|
MZIP10a-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-10b anti-miR-10b microRNA construct |
|
MZIP10b-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-122 anti-miR-122 microRNA construct |
|
MZIP122-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-124 anti-miR-124 microRNA construct |
|
MZIP124-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-128 anti-miR-128 microRNA construct |
|
MZIP128-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-132 anti-miR-132 microRNA construct |
|
MZIP132-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-134 anti-miR-134 microRNA construct |
|
MZIP134-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-136 anti-miR-136 microRNA construct |
|
MZIP136-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-137 anti-miR-137 microRNA construct |
|
MZIP137-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-138 anti-miR-138 microRNA construct |
|
MZIP138-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-141 anti-miR-141 microRNA construct |
|
MZIP141-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-143 anti-miR-143 microRNA construct |
|
MZIP143-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-144 anti-miR--144 microRNA construct |
|
MZIP144-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-145 anti-miR-145 microRNA construct |
|
MZIP145-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-147 anti-miR-147 microRNA construct |
|
MZIP147-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-149 anti-miR-149 microRNA construct |
|
MZIP149-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-150 anti-miR-150 microRNA construct |
|
MZIP150-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-152 anti-miR-152 microRNA construct |
|
MZIP152-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-153 anti-miR-153 microRNA construct |
|
MZIP153-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-154 anti-miR-154 microRNA construct |
|
MZIP154-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-155 anti-miR-155 microRNA construct |
|
MZIP155-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-15a anti-miR-15a microRNA construct |
|
MZIP15a-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-15b anti-miR-15b microRNA construct |
|
MZIP15b-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-182 anti-miR-182 microRNA construct |
|
MZIP182-AA-1 |
SBI |
Miniprep DNA |
EUR 620 |
miRZip-182 anti-miR-182 microRNA construct |
|
MZIP182-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-183 anti-miR-183 microRNA construct |
|
MZIP183-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-184 anti-miR-184 microRNA construct |
|
MZIP184-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-185 anti-miR-185 microRNA construct |
|
MZIP185-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-186 anti-miR-186 microRNA construct |
|
MZIP186-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-187 anti-miR-187 microRNA construct |
|
MZIP187-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-18a anti-miR-18a microRNA construct |
|
MZIP18a-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-18b anti-miR-18b microRNA construct |
|
MZIP18b-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-190 anti-miR-190 microRNA construct |
|
MZIP190-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-192 anti-miR-192 microRNA construct |
|
MZIP192-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-194 anti-miR-194 microRNA construct |
|
MZIP194-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-195 anti-miR-195 microRNA construct |
|
MZIP195-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-198 anti-miR-198 microRNA construct |
|
MZIP198-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-19a anti-miR-19a microRNA construct |
|
MZIP19a-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-19b anti-miR-19b microRNA construct |
|
MZIP19b-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-202 anti-miR-202 microRNA construct |
|
MZIP202-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-203 anti-miR-203 microRNA construct |
|
MZIP203-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-204 anti-miR-204 microRNA construct |
|
MZIP204-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-205 anti-miR-205 microRNA construct |
|
MZIP205-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-206 anti-miR-206 microRNA construct |
|
MZIP206-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-20a anti-miR-20a microRNA construct |
|
MZIP20a-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-20b anti-miR-20b microRNA construct |
|
MZIP20b-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-210 anti-miR-210 microRNA construct |
|
MZIP210-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-211 anti-miR-211 microRNA construct |
|
MZIP211-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-214 anti-miR-214 microRNA construct |
|
MZIP214-AA-1 |
SBI |
Miniprep DNA |
EUR 620 |
miRZip-214 anti-miR-214 microRNA construct |
|
MZIP214-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-217 anti-miR-217 microRNA construct |
|
MZIP217-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-218 anti-miR-218 microRNA construct |
|
MZIP218-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-221 anti-miR-221 microRNA construct |
|
MZIP221-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-222 anti-miR-222 microRNA construct |
|
MZIP222-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-223 anti-miR-223 microRNA construct |
|
MZIP223-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-224 anti-miR-224 microRNA construct |
|
MZIP224-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-23a anti-miR-23a microRNA construct |
|
MZIP23a-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-23b anti-miR-23b microRNA construct |
|
MZIP23b-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-26a anti-miR-26a microRNA construct |
|
MZIP26a-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-26b anti-miR-26b microRNA construct |
|
MZIP26b-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-27a anti-miR-27a microRNA construct |
|
MZIP27a-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-27b anti-miR-27b microRNA construct |
|
MZIP27b-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-29a anti-miR-29a microRNA construct |
|
MZIP29a-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-29b anti-miR-29b microRNA construct |
|
MZIP29b-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-29c anti-miR-29c microRNA construct |
|
MZIP29c-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-30a anti-miR-30a microRNA construct |
|
MZIP30a-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-30b anti-miR-30b microRNA construct |
|
MZIP30b-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-30c anti-miR-30c microRNA construct |
|
MZIP30c-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-30d anti-miR-30d microRNA construct |
|
MZIP30d-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
 miRZip-30e anti-miR-30e microRNA construct |
|
MZIP30e-PA-1 |
SBI |
Bacterial Streak |
EUR 620 |
For Pol III sort 2 promoters, we discovered that the shorter poly-U tract lengths of four uracils had been ample to induce termination. These findings reveal a key function for sequence and structural parts inside Pol III-transcribed nascent RNA for environment friendly transcription termination, and reveal a generalizable assay for characterizing Pol III transcription in human cells.
