Epigenetics Unit of the Human Genetics Foundation (HuGeF)

CIRS-seq: Chemical Inference of RNA Structure

Structure of TFIIK for phosphorylation of CTD of RNA polymerase II

By Jade Pig
Structure of TFIIK for phosphorylation of CTD of RNA polymerase II
Throughout transcription initiation, the final transcription issue TFIIH marks RNA polymerase II by phosphorylating Ser5 of the carboxyl-terminal area (CTD) of Rpb1, which is adopted by in depth modifications coupled to transcription elongation, mRNA processing, and histone dynamics. We now have decided a 3.5-Å decision cryo-electron microscopy (cryo-EM) construction of the TFIIH kinase module (TFIIK in yeast), which consists of Kin28, Ccl1, and Tfb3, yeast homologs of CDK7, cyclin H, and MAT1, respectively. The carboxyl-terminal area of Tfb3 was mendacity on the fringe of catalytic cleft of Kin28, the place a conserved Tfb3 helix served to stabilize the activation loop in its lively conformation. By combining the construction of TFIIK with the earlier cryo-EM construction of the preinitiation advanced, we prolong the beforehand proposed mannequin of the CTD path to the lively web site of TFIIK.

The construction of Prp2 sure to RNA and ADP-BeF 3– reveals structural options necessary for RNA unwinding by DEAH-box ATPases

Noncoding intron sequences current in precursor mRNAs have to be eliminated previous to translation, and they’re excised by way of the spliceosome, a multimegadalton molecular machine composed of quite a few protein and RNA parts. The DEAH-box ATPase Prp2 performs an important function throughout pre-mRNA splicing because it ensures the catalytic activation of the spliceosome. Regardless of excessive structural similarity to different spliceosomal DEAH-box helicases, Prp2 doesn’t appear to operate as an RNA helicase, however fairly as an RNA-dependent ribonucleoprotein particle-modifying ATPase. Latest crystal buildings of the spliceosomal DEAH-box ATPases Prp43 and Prp22, in addition to of the associated RNA helicase MLE, in advanced with RNA have contributed to a greater understanding of how RNA binding and processivity may be achieved on this helicase household.
As a way to shed mild onto the divergent method of operate of Prp2, an N-terminally truncated assemble of Chaetomium thermophilum Prp2 was crystallized within the presence of ADP-BeF3– and a poly-U12 RNA. The refined construction revealed a just about equivalent conformation of the helicase core in contrast with the ADP-BeF3–– and RNA-bound construction of Prp43, and solely a minor shift of the C-terminal domains. Nonetheless, Prp2 and Prp43 differ within the hook-loop and a loop of the helix-bundle area, which interacts with the hook-loop and evokes a unique RNA conformation instantly after the three’ stack. On changing these loop residues in Prp43 by the Prp2 sequence, the unwinding exercise of Prp43 was abolished. Moreover, a putative exit tunnel for the γ-phosphate after ATP hydrolysis could possibly be recognized in one of many Prp2 buildings.

Combinatorial optimization of mRNA construction, stability, and translation for RNA-based therapeutics

Therapeutic mRNAs and vaccines are being developed for a broad vary of human illnesses, together with COVID-19. Nonetheless, their optimization is hindered by mRNA instability and inefficient protein expression. Right here, we describe design ideas that overcome these obstacles. We develop a brand new RNA sequencing-based platform known as PERSIST-seq to systematically delineate in-cell mRNA stability, ribosome load, in addition to in-solution stability of a library of various mRNAs.
We discover that, surprisingly, in-cell stability is a higher driver of protein output than excessive ribosome load. We additional introduce a technique known as In-line-seq, utilized to hundreds of various RNAs, that reveals sequence and structure-based guidelines for mitigating hydrolytic degradation. Our findings present that “superfolder” mRNAs might be designed to enhance each stability and expression which are additional enhanced by pseudouridine nucleoside modification. Collectively, our research demonstrates simultaneous enchancment of mRNA stability and protein expression and offers a computational-experimental platform for the enhancement of mRNA medicines.

Small molecule focusing on of biologically related RNA tertiary and quaternary buildings

Preliminary successes in creating small molecule ligands for non-coding RNAs have underscored their potential as therapeutic targets. Extra lately, these successes have been aided by advances in biophysical and structural methods for identification and characterization of extra advanced RNA buildings; these higher-level folds current protein-like binding pockets that provide alternatives to design small molecules that might obtain a level of selectivity typically onerous to acquire on the main and secondary construction degree. Extra particularly, identification and small molecule focusing on of RNA tertiary and quaternary buildings have allowed researchers to probe a number of human illnesses and have resulted in promising scientific candidates.
On this evaluation we spotlight a number of various and thrilling successes and the experimental approaches that led to their discovery. These research embody examples of latest developments in RNA-centric assays and ligands that present perception into the options liable for the affinity and organic consequence of RNA-targeted chemical probes. This report highlights the potential and rising alternatives to selectively goal RNA tertiary and quaternary buildings as a route to raised perceive and, in the end, deal with many illnesses.
Structure of TFIIK for phosphorylation of CTD of RNA polymerase II

RNA sequence and construction determinants of Pol III transcriptional termination in human cells

The exact mechanism of transcription termination of the eukaryotic RNA polymerase III (Pol III) has been a topic of appreciable debate. Though earlier research have clearly proven that a number of uracils on the finish of RNA transcripts are required for Pol III termination, the consequences of upstream RNA secondary construction within the nascent transcript on transcriptional termination remains to be unclear. To handle this, we developed an in cellulo Pol III transcription termination assay utilizing the lately developed Twister-Corn RNA aptamer system to create a Pol III-transcribed RNA that produces a detectable fluorescent sign when transcribed in human cells.
To review the consequences of RNA sequence and construction on Pol III termination, we systematically different the sequence context upstream of the aptamer and recognized sequence traits that improve or diminish termination. For transcription from Pol III sort Three promoters, we discovered that solely poly-U tracts longer than the common size discovered within the human genome effectively terminate Pol III transcription with out RNA secondary construction parts. We noticed that RNA secondary construction parts positioned in proximity to shorter poly-U tracts induced termination, and RNA secondary construction by itself was not ample to induce termination.

Monkey Cancer/testis antigen 47B(CT47B1) ELISA kit
E09C2139-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen 47B(CT47B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cancer/testis antigen 47B(CT47B1) ELISA kit
E09C2139-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen 47B(CT47B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cancer/testis antigen 1(CTAG1A) ELISA kit
E09C2140-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 1(CTAG1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cancer/testis antigen 1(CTAG1A) ELISA kit
E09C2140-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 1(CTAG1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cancer/testis antigen 1(CTAG1A) ELISA kit
E09C2140-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 1(CTAG1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cancer/testis antigen 2(CTAG2) ELISA kit
E09C2141-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 2(CTAG2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cancer/testis antigen 2(CTAG2) ELISA kit
E09C2141-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 2(CTAG2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cancer/testis antigen 2(CTAG2) ELISA kit
E09C2141-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 2(CTAG2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Bromodomain testis specific protein(BRDT) ELISA kit
E09B0840-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Monkey Bromodomain testis specific protein(BRDT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Bromodomain testis specific protein(BRDT) ELISA kit
E09B0840-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Monkey Bromodomain testis specific protein(BRDT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Bromodomain testis specific protein(BRDT) ELISA kit
E09B0840-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Monkey Bromodomain testis specific protein(BRDT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse ; Testis
02-715 100ng
EUR 401
Description: The Mouse ; Testis is available in Europe and for worldwide shipping via Gentaur.

Testis Lysate
1416 0.1 mg
EUR 191
Description: Testis tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Testis Lysate
1476 0.1 mg
EUR 191
Description: Testis tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Testis Lysate
1313 0.1 mg
EUR 191
Description: Testis tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Monkey Testis expressed sequence 10 protein(TEX10) ELISA kit
E09T0680-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Monkey Testis expressed sequence 10 protein(TEX10) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Testis expressed sequence 10 protein(TEX10) ELISA kit
E09T0680-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Monkey Testis expressed sequence 10 protein(TEX10) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Testis expressed sequence 10 protein(TEX10) ELISA kit
E09T0680-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Monkey Testis expressed sequence 10 protein(TEX10) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Testis Membrane Lysate
XBL-10957 0.1 mg
EUR 516.5
Description: Human testis tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human testis tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated testis tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated testis tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Testis Tumor Lysate
1381 0.1 mg
EUR 254
Description: Testis tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Monkey Cancer/testis antigen family 45 member A1(CT45A1) ELISA kit
E09C2133-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A1(CT45A1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cancer/testis antigen family 45 member A1(CT45A1) ELISA kit
E09C2133-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A1(CT45A1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cancer/testis antigen family 45 member A1(CT45A1) ELISA kit
E09C2133-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A1(CT45A1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cancer/testis antigen family 45 member A2(CT45A2) ELISA kit
E09C2134-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A2(CT45A2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cancer/testis antigen family 45 member A2(CT45A2) ELISA kit
E09C2134-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A2(CT45A2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cancer/testis antigen family 45 member A2(CT45A2) ELISA kit
E09C2134-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A2(CT45A2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cancer/testis antigen family 45 member A3(CT45A3) ELISA kit
E09C2135-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A3(CT45A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cancer/testis antigen family 45 member A3(CT45A3) ELISA kit
E09C2135-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A3(CT45A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cancer/testis antigen family 45 member A3(CT45A3) ELISA kit
E09C2135-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A3(CT45A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cancer/testis antigen family 45 member A5(CT45A5) ELISA kit
E09C2137-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A5(CT45A5) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cancer/testis antigen family 45 member A5(CT45A5) ELISA kit
E09C2137-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A5(CT45A5) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cancer/testis antigen family 45 member A5(CT45A5) ELISA kit
E09C2137-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A5(CT45A5) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Fetal and adult testis expressed transcript protein(FATE1) ELISA kit
E09F0361-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Monkey Fetal and adult testis expressed transcript protein(FATE1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Fetal and adult testis expressed transcript protein(FATE1) ELISA kit
E09F0361-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Monkey Fetal and adult testis expressed transcript protein(FATE1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Fetal and adult testis expressed transcript protein(FATE1) ELISA kit
E09F0361-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Monkey Fetal and adult testis expressed transcript protein(FATE1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Testis Tissue Lysate
LYSATE0005 200ug
EUR 150
Description: This cell lysate is prepared from rat testis tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Mouse Testis Tissue Lysate
LYSATE0014 200ug
EUR 150
Description: This cell lysate is prepared from mouse testis tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Mouse Testis Nuclear Extract
X12009 500 µg Ask for price
Description: kits suitable for this type of research

Human Testis Tumor lysate
HTL-1381 1 mg
EUR 773

Testis Tissue Slide (Normal)
11-701-10um 10 um
EUR 201.5

Testis Tissue Slide (Normal)
11-701-4um 4 um
EUR 180.5

Testis Tissue Slide (Abnormal)
11-710-10um 10 um
EUR 201.5

Testis Tissue Slide (Abnormal)
11-710-4um 4 um
EUR 180.5

Testis Liver Cirrhosis Lysate
XBL-10389 0.1 mg
EUR 663.5
Description: Human testis tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human testis tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the testis tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The testis tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Testis Membrane Tumor Lysate
XBL-10966 0.1 mg
EUR 626.75
Description: Human testis tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human testis tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated testis tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated testis tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) ELISA kit
E09C2136-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) ELISA kit
E09C2136-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) ELISA kit
E09C2136-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Testis Tumor Tissue Array - 16 cases of testis tumors and 8 cases of non-tumor testis tissues.
Z7020096 5 slides
EUR 1381
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

MicroRNA Isolation Kit
KS341025 1 kit
EUR 256
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Testis Expressed 10 (TEX10) Antibody
20-abx116034
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Testis Expressed 11 (TEX11) Antibody
20-abx116035
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Testis Expressed 12 (TEX12) Antibody
20-abx116036
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Testis Expressed 13A (TEX13A) Antibody
20-abx116037
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Testis Expressed 14 (TEX14) Antibody
20-abx116038
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Testis Specific, 10 (TSGA10) Antibody
20-abx116039
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Testis Expressed 13A (TEX13A) Antibody
20-abx311757
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Testis Specific 10 (TSGA10) Antibody
20-abx003819
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul

Recombinant Human Testis Derived Transcript
7-06631 2µg Ask for price

Recombinant Human Testis Derived Transcript
7-06632 10µg Ask for price

Recombinant Human Testis Derived Transcript
7-06633 1mg Ask for price

Testis Specific 10 (TSGA10) Antibody
abx145551-100ug 100 ug
EUR 391

Testis Expressed 12 (TEX12) Antibody
abx030409-400ul 400 ul
EUR 523

Testis Expressed 12 (TEX12) Antibody
abx030409-80l 80 µl
EUR 286

Testis Specific 10 (TSGA10) Antibody
abx031103-400ul 400 ul
EUR 523

Testis Specific 10 (TSGA10) Antibody
abx031103-80l 80 µl
EUR 286

Testis Expressed 11 (TEX11) Antibody
abx433354-200ul 200 ul
EUR 286

Testis Expressed 10 (TEX10) Antibody
abx238604-100ug 100 ug
EUR 509

Testis Expressed 101 (TEX101) Antibody
abx238605-100ug 100 ug
EUR 551

Testis Expressed 11 (TEX11) Antibody
abx238606-100ug 100 ug
EUR 509

Testis Expressed 12 (TEX12) Antibody
abx238607-100ug 100 ug
EUR 509

Testis Expressed 13A (TEX13A) Antibody
abx238608-100ug 100 ug
EUR 551

Testis Expressed 264 (TEX264) Antibody
abx238611-100ug 100 ug
EUR 509

Testis Expressed 38 (TEX38) Antibody
abx238613-100ug 100 ug
EUR 509

Testis Specific 10 (TSGA10) Antibody
abx239042-100ug 100 ug
EUR 509

Testis Specific 13 (TSGA13) Antibody
abx239043-100ug 100 ug
EUR 551

Testis Specific 13 (TSGA13) Antibody
20-abx302813
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Testis Specific 10 (TSGA10) Antibody
20-abx302841
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Rat Testis Whole tissue lysate
RAL-1476 1 mg
EUR 524

Mouse Testis Whole tissue lysate
MAL-1416 1 mg
EUR 524

Human Adult cDNA Tissue: Testis
HA-260 10 rxn
EUR 415

Cancer MicroRNA qPCR Array
RA610A-1 10 profiles
EUR 1172

Human Cancer/testis antigen 1 (CTAG1A)
1-CSB-YP006125HU
  • EUR 430.00
  • EUR 234.00
  • EUR 1508.00
  • EUR 642.00
  • EUR 1009.00
  • EUR 291.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Cancer/testis antigen 1(CTAG1A) expressed in Yeast

Human Cancer/testis antigen 2 (CTAG2)
1-CSB-YP006127HUa4
  • EUR 679.00
  • EUR 335.00
  • EUR 2172.00
  • EUR 1051.00
  • EUR 1442.00
  • EUR 435.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Cancer/testis antigen 2(CTAG2) expressed in Yeast

Recombinant Cancer/Testis Antigen 1B (CTAG1B)
4-RPJ316Hu01
  • EUR 490.66
  • EUR 234.00
  • EUR 1564.96
  • EUR 588.32
  • EUR 1076.64
  • EUR 391.00
  • EUR 3762.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Cancer/Testis Antigen 1B expressed in: E.coli

Recombinant Testis Anion Transporter 1 (TAT1)
4-RPE478Hu01
  • EUR 494.24
  • EUR 235.00
  • EUR 1578.40
  • EUR 592.80
  • EUR 1085.60
  • EUR 394.00
  • EUR 3796.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Testis Anion Transporter 1 expressed in: E.coli

Recombinant Testis Specific Kinase 1 (TESK1)
4-RPC789Hu01
  • EUR 472.74
  • EUR 229.00
  • EUR 1497.76
  • EUR 565.92
  • EUR 1031.84
  • EUR 379.00
  • EUR 3594.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Testis Specific Kinase 1 expressed in: E.coli

Recombinant Testis Specific Kinase 1 (TESK1)
4-RPC789Mu01
  • EUR 499.62
  • EUR 236.00
  • EUR 1598.56
  • EUR 599.52
  • EUR 1099.04
  • EUR 397.00
  • EUR 3846.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Testis Specific Kinase 1 expressed in: E.coli

Human Cancer/testis antigen 1 (CTAG1A)
1-CSB-EP006125HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Cancer/testis antigen 1(CTAG1A) expressed in E.coli

Human Cancer/testis antigen 2 (CTAG2)
1-CSB-EP006127HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Cancer/testis antigen 2(CTAG2) expressed in E.coli

Testis Derived Transcript Human Recombinant Protein
PROTQ9UGI8 Regular: 10ug
EUR 317
Description: TES Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 441amino acids (1-421 a.a.) and having a molecular mass of 50.1 kDa. The TES protein is fused to 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Cancer/Testis Antigen 1A (CTAG1A) Antibody
20-abx126874
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul

Cancer/Testis Antigen 1B (CTAG1B) Antibody
20-abx128209
  • EUR 425.00
  • EUR 133.00
  • EUR 1205.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Testis Anion Transporter 1 (TAT1) Antibody
20-abx129200
  • EUR 425.00
  • EUR 133.00
  • EUR 1205.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Testis Specific Kinase 1 (TESK1) Antibody
20-abx130977
  • EUR 439.00
  • EUR 133.00
  • EUR 1233.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Testis Specific Kinase 1 (TESK1) Antibody
20-abx130978
  • EUR 425.00
  • EUR 133.00
  • EUR 1205.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Testis-expressed sequence 12 protein Antibody
20-abx110259
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Phosphorylase Kinase, gamma 2 (Testis) Antibody
20-abx114507
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Cancer/Testis Antigen 1A (CTAG1A) Antibody
20-abx111352
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Human Testis PrimaCell: Normal Sertoli Cells
2-96105 1 Kit Ask for price

Testis Specific 13 (TSGA13) Antibody (HRP)
20-abx309618
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Testis Specific 13 (TSGA13) Antibody (FITC)
20-abx309619
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Testis Specific 13 (TSGA13) Antibody (Biotin)
20-abx309620
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Testis Specific 10 (TSGA10) Antibody (HRP)
20-abx310740
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Testis Specific 10 (TSGA10) Antibody (FITC)
20-abx310741
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Testis Specific 10 (TSGA10) Antibody (Biotin)
20-abx310742
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Testis Expressed 13A (TEX13A) Antibody (HRP)
20-abx311758
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Testis Expressed 13A (TEX13A) Antibody (FITC)
20-abx311759
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Testis Expressed 13A (TEX13A) Antibody (Biotin)
20-abx311760
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Cancer/Testis Antigen 5.1 (SSX1) Antibody
20-abx323587
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

Testis-Expressed Protein 35 (Tex35) Antibody
20-abx307697
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Human Testis OptiTDS: Tissue Dissociation System
4-28105 1 Kit Ask for price

Cancer/Testis Antigen 5.1 (SSX1) Antibody
abx145140-100ug 100 ug
EUR 391

Cancer/testis Antigen 2 (CTAG2) Antibody
20-abx149592
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug

Cancer/Testis Antigen 1B (CTAG1B) Antibody
20-abx135754
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Testis Specific Kinase 1 (TESK1) Antibody
20-abx008652
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Bromodomain Testis-Specific Protein (BRDT) Antibody
abx025117-400ul 400 ul
EUR 523

Bromodomain Testis-Specific Protein (BRDT) Antibody
abx025117-80l 80 µl
EUR 286

Testis-Expressed Protein 9 (TEX9) Antibody
abx025800-400ul 400 ul
EUR 523

Testis-Expressed Protein 9 (TEX9) Antibody
abx025800-80l 80 µl
EUR 286

Testis-Expressed Protein 13B (TEX13B) Antibody
abx025974-400ul 400 ul
EUR 523

Testis-Expressed Protein 13B (TEX13B) Antibody
abx025974-80l 80 µl
EUR 286

Testis Serine Protease 1 (TESSP1) Antibody
abx026869-400ul 400 ul
EUR 523

Testis Serine Protease 1 (TESSP1) Antibody
abx026869-80l 80 µl
EUR 286

Cancer/Testis Antigen 1A (CTAG1A) Antibody
abx027441-400ul 400 ul
EUR 523

Cancer/Testis Antigen 1A (CTAG1A) Antibody
abx027441-80l 80 µl
EUR 286

Testis-Expressed Protein 33 (EAN57) Antibody
abx029059-400ul 400 ul
EUR 523

Testis-Expressed Protein 33 (EAN57) Antibody
abx029059-80l 80 µl
EUR 286

Testis Specific Kinase 1 (TESK1) Antibody
abx029069-400ul 400 ul
EUR 523

Testis Specific Kinase 1 (TESK1) Antibody
abx029069-80l 80 µl
EUR 286

Testis-Expressed Protein 2 (TEX2) Antibody
abx029879-400ul 400 ul
EUR 523

Testis-Expressed Protein 2 (TEX2) Antibody
abx029879-80l 80 µl
EUR 286

Cancer/testis Antigen 2 (CTAG2) Antibody
abx027593-400ul 400 ul
EUR 523

Cancer/testis Antigen 2 (CTAG2) Antibody
abx027593-80l 80 µl
EUR 286

Cancer/Testis Antigen 5.1 (SSX1) Antibody
abx238257-100ug 100 ug
EUR 551

Testis Anion Transporter 1 (TAT1) Antibody
20-abx174745
  • EUR 857.00
  • EUR 439.00
  • 1 mg
  • 200 ug
For Pol III sort 2 promoters, we discovered that the shorter poly-U tract lengths of four uracils had been ample to induce termination. These findings reveal a key function for sequence and structural parts inside Pol III-transcribed nascent RNA for environment friendly transcription termination, and reveal a generalizable assay for characterizing Pol III transcription in human cells.

Jade

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