NMR solution structures of Runella slithyformis RNA 2′-phosphotransferase Tpt1 provide insights into NAD+ binding and specificity

NMR solution structures of Runella slithyformis RNA 2'-phosphotransferase Tpt1 provide insights into NAD+ binding and specificity

Tpt1, an integral part of the fungal and plant tRNA splicing equipment, catalyzes switch of an inside RNA 2′-PO4 to NAD+ yielding RNA 2′-OH and ADP-ribose-1′,2′-cyclic phosphate merchandise. Right here, we report NMR buildings of the Tpt1 ortholog from the bacterium Runella slithyformis (RslTpt1), as apoenzyme and certain to NAD+. RslTpt1 consists of N- and C-terminal lobes with substantial inter-lobe dynamics within the free and NAD+-bound states. ITC measurements of RslTpt1 binding to NAD+ (KD ∼31 μM), ADP-ribose (∼96 μM) and ADP (∼123 μM) point out that substrate affinity is decided primarily by the ADP moiety; no binding of NMN or nicotinamide is noticed by ITC.

NAD+-induced chemical shift perturbations (CSPs) localize completely to the RslTpt1 C-lobe. NADP+, which accommodates an adenylate 2′-PO4 (mimicking the substrate RNA 2′-PO4), binds with decrease affinity (KD ∼1 mM) and elicits solely N-lobe CSPs. The RslTpt1·NAD+ binary advanced reveals C-lobe contacts to adenosine ribose hydroxyls (His99, Thr101), the adenine nucleobase (Asn105, Asp112, Gly113, Met117) and the nicotinamide riboside (Ser125, Gln126, Asn163, Val165), a number of of that are important for RslTpt1 exercise in vivo. Proximity of the NAD+ β-phosphate to ribose-C1″ means that it could stabilize an oxocarbenium transition-state throughout step one of the Tpt1-catalyzed response.

Reverse roles of transcription elongation components Spt4/5 and Elf1 in RNA polymerase II transcription by way of B-form versus non-B DNA buildings

Transcription elongation may be affected by quite a few forms of obstacles, resembling nucleosome, pausing sequences, DNA lesions and non-B-form DNA buildings. Spt4/5 and Elf1 are conserved transcription elongation components that promote RNA polymerase II (Pol II) bypass of nucleosome and pausing sequences. Importantly, genetic research have proven that Spt4/5 performs important roles within the transcription of expanded nucleotide repeat genes related to inherited neurological illnesses. Right here, we examine the operate of Spt4/5 and Elf1 within the transcription elongation of CTG•CAG repeat utilizing an in vitro reconstituted yeast transcription system.
We discovered that Spt4/5 helps Pol II transcribe by way of the CTG•CAG tract duplex DNA, which is in good settlement with its canonical roles in stimulating transcription elongation. In sharp distinction, surprisingly, we revealed that Spt4/5 enormously inhibits Pol II transcriptional bypass of CTG and CAG slip-out buildings. Moreover, we demonstrated that transcription elongation issue Elf1 individually and cooperatively with Spt4/5 inhibits Pol II bypass of the slip-out buildings. This research uncovers the vital purposeful interplays between template DNA buildings and the operate of transcription elongation components. This research additionally expands our understanding of the capabilities of Spt4/5 and Elf1 in transcriptional processing of trinucleotide repeat DNA.

Scoring Capabilities for Protein-RNA Advanced Construction Prediction: Advances, Functions, and Future Instructions

Protein-RNA interplay is among the many most important of organic occasions in dwelling cells, being concerned in protein synthesizing, RNA processing and transport, DNA transcription, and regulation of gene expression, and lots of different important bio-molecular actions. A radical understanding of this interplay is of paramount significance in elementary research of a wide range of important mobile processes and therapeutic utility for treatment of a broad vary of illnesses. Experimental high-resolution 3D construction willpower is the first supply of information for protein-RNA complexes.
Nevertheless, attributable to technical limitations, the prevailing methods for experimental construction willpower could not match the demand from quick rising curiosity in academia and business. This downside necessitates the choice high-throughput computational technique for protein-RNA advanced construction prediction. Just like the in silico strategies used for protein-protein and protein-DNA interactions, a dependable prediction of protein-RNA advanced construction requires a scoring operate with commensurate discriminatory energy.
Derived from decided buildings and purposed to foretell the to-be-determined buildings, the scoring operate shouldn’t be solely a predictive instrument but in addition a gauge of our data of protein-RNA interplay. On this overview, we current an outline of the standing of current scoring capabilities and the scientific precept behind their constructions in addition to their strengths and limitations. Lastly, we’ll talk about about future instructions of the scoring operate growth for protein-RNA construction prediction.

Meeting and Cryo-EM construction willpower of yeast mitochondrial RNA polymerase initiation advanced intermediates

In yeast mitochondria, transcription initiation requires meeting of mitochondrial RNA polymerase and transcription initiation issue MTF1 on the DNA promoter initiation web site. This protocol describes the purification of the part proteins and meeting of partially melted and totally melted initiation advanced states. Each states co-exist in equilibrium in the identical pattern as seen by cryoelectron microscopy (cryo-EM) and permit elucidation of MTF1’s structural roles in controlling the transition into elongation. We additional define how evaluation of the advanced by mild scattering, thermal shift assay, and ultrafiltration assay reveals reproducible outcomes. For full particulars on the use and execution of this protocol, please discuss with De Wijngaert et al. (2021).
NMR solution structures of Runella slithyformis RNA 2'-phosphotransferase Tpt1 provide insights into NAD+ binding and specificity

Construction-guided engineering of adenine base editor with minimized RNA off-targeting exercise

Each adenine base editors (ABEs) and cytosine base editors (CBEs) have been lately revealed to induce transcriptome-wide RNA off-target enhancing in a information RNA-independent method. Right here we assemble a reporter system containing E.coli Hokb gene with a tRNA-like motif for strong detection of RNA enhancing actions because the optimized ABE, ABEmax, induces extremely environment friendly A-to-I (inosine) enhancing inside an E.coli tRNA-like construction. Then, we design mutations to disrupt the potential interplay between TadA and tRNAs in structure-guided ideas and discover that Arginine 153 (R153) inside TadA is important for deaminating RNAs with core tRNA-like buildings.

Pancreas Lysate

1414 0.1 mg
EUR 229.2
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Pancreas Lysate

1469 0.1 mg
EUR 229.2
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

cDNA - Lupus: Pancreas

C1236188Lup 40 reactions
EUR 737

CK PANCREAS 25 EA*

43031-2 25 EA
EUR 354.66

Stem Cell MicroRNA Profiling Panel

RA620A-1 10 profiles
EUR 1406.4

Pancreas Tumor Lysate

1334 0.1 mg
EUR 304.8
Description: Pancreas tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Pancreas Lupus Lysate

XBL-10373 0.1 mg
EUR 796.2
Description: Human pancreas tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human pancreas tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the pancreas tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The pancreas tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Pancreas disease spectrum (pancreatic cancer progression) with pancreas tissue array

PA2081c each
EUR 594
Description: Pancreas disease spectrum (pancreatic cancer progression) with pancreas tissue array, including pathology grade, TNM and clinical stage, 96 cases/192 cores, replacing PA2081b

Pig PANCREAS 10 EA*

59431-2 10 EA
EUR 382.07

Trypsin, Human Pancreas

7292-50 each
EUR 373.2

Trypsin, Human Pancreas

TRYP15-N-50 50 ug
EUR 270

Pancreas Membrane Lysate

XBL-10780 0.1 mg
EUR 619.8
Description: Human pancreas tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Elastase, Porcine Pancreas

P1276-250 each
EUR 261.6

MicroRNA Isolation Kit

KS341025 1 kit
EUR 247

Chymotrypsin, Human Pancreas

7538-100 each
EUR 438

Chymotrypsin, Human Pancreas

CHYT15-N-100 100 ug
EUR 343.2

Pancreas Cytoplasmic Lysate

XBL-10779 0.1 mg
EUR 273.3
Description: Human pancreas tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

Total RNA - Lupus: Pancreas

R1236188Lup-50 50 ug
EUR 421.2

Rabbit PANCREAS Y 25 EA*

41231-2 25 EA
EUR 409.48

Human Pancreas Tumor lysate

HTL-1334 1 mg
EUR 927.6

Pancreas Tissue Slide (Normal)

11-101-10um 10 um
EUR 241.8

Pancreas Tissue Slide (Normal)

11-101-4um 4 um
EUR 216.6

Pancreas Tissue Lysate (Tumor)

1737-01 0.1 mg
EUR 336.3
Description: Pancreas tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Pancreas Tissue Lysate (Tumor)

1737-02 0.1 mg
EUR 336.3
Description: Pancreas tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Pancreas Tissue Lysate (Tumor)

1738-01 0.1 mg
EUR 336.3
Description: Pancreas tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Human Pancreas Tissue Lysate

30R-AP030 150 ug
EUR 262.8
Description: Fresh tissue lysate isolated from human pancreas

Insulin from bovine pancreas

abx082234-25mg 25 mg
EUR 260.4

Total Protein - Lupus: Pancreas

P1236188Lup 1 mg
EUR 486

Pancreas Tissue Lysate (Normal)

1736-02 0.1 mg
EUR 260.7
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Pancreas Tissue Lysate (Normal)

1736-05 0.1 mg
EUR 260.7
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

cDNA - Liver Cirrhosis: Pancreas

C1236188Lcs 40 reactions
EUR 729

CK PANCREAS POWDER LYOPH 1g*

43032-0 1g
EUR 147.17

Cancer MicroRNA qPCR Array

RA610A-1 10 profiles
EUR 1406.4

Pancreas Tissue Slide (Abnormal)

11-106-10um 10 um
EUR 241.8

Pancreas Tissue Slide (Abnormal)

11-106-4um 4 um
EUR 216.6

Elastase from Porcine Pancreas

30047 1 mg
EUR 119.16
Description: Elastase from Porcine Pancreas

Alpha-Amylase, Human Pancreas

P1454-100 each
EUR 444

Alpha-Amylase, Human Pancreas

P1545-100 100 units
EUR 417.6

Pancreas Membrane Tumor Lysate

XBL-10795 0.1 mg
EUR 1023
Description: Human pancreas tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

CK PANCREAS POWDER LYOPH 10g*

43032-1 10g
EUR 400.33

CK PANCREAS POWDER LYOPH 100g*

43032-2 100g
EUR 2981.63

Rabbit PANCREAS MATURE 25 EA*

41331-2 25 EA
EUR 409.48

Human microRNA-210(miR-210)ELISA

QY-E05678 96T
EUR 433.2

Pancreas Liver Cirrhosis Lysate

XBL-10372 0.1 mg
EUR 796.2
Description: Human pancreas tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human pancreas tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the pancreas tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The pancreas tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Cancer of the pancreas, 102 cases (1.5mm)

PAC1021 1
EUR 354
Description: Pancreatic cancer tissue array contains 96 cases of pancreatic cancers and 6 cases of normal and non-malignant pancreatic tissues with grading and TNM staging data.

Fetal Pancreas Cytoplasmic Lysate

XBL-10791 0.1 mg
EUR 273.3
Description: Human pancreas tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The fetal human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

Pancreas Cytoplasmic Tumor Lysate

XBL-10794 0.1 mg
EUR 273.3
Description: Human pancreas tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

Pancreatic cancer with pancreas tissue array

PA2082a each
EUR 546
Description: Pancreatic cancer with pancreas tissue array, including ductal adenocarcinoma(PADC),acinar cell carcinoma(PACC),normal adjacent and pancreas tissue,pathology grade, TNM and clinical stage, 96 cases/192 cores(1.0mm), replacing PA2082

Pancreatic cancer with pancreas tissue array

PA805c each
EUR 306
Description: Pancreatic cancer with pancreas tissue array, including duct adenocarcinoma(PDAC), acinar cell carcinoma(PACC),squamous cell carcinoma,normal adjacent pancreas and normal tissuepathology grade, TNM and clinical stage, 80 cases/80 cores(1.5mm), replacing PA805b

Pancreatic cancer with pancreas tissue array

T141c each
EUR 48
Description: Pancreatic cancer with pancreas tissue array, including pathology grade, TNM and clinical stage, 6 cases/24 cores, replacing T141b

Human Pancreas Chymotrypsin Protein

abx060937-100ug 100 ug
EUR 577.2

cDNA - Human Tumor Tissue: Pancreas

C1235188-10 10 reactions
EUR 274

Rat Pancreas Whole tissue lysate

RAL-1469 1 mg
EUR 628.8

Rat Pancreas PrimaCell: Normal Pancreatic Islets

2-82578 1 Kit Ask for price

Pancreas Tissue Slide (Adenocarcinoma)

11-105-10um 10 um
EUR 241.8

Pancreas Tissue Slide (Adenocarcinoma)

11-105-4um 4 um
EUR 216.6

Pig (Pancreas) alpha Amylase Enzyme

abx070001-25kU 25 kU
EUR 777.6

Mouse Pancreas Whole tissue lysate

MAL-1414 1 mg
EUR 628.8

Total RNA - Liver Cirrhosis: Pancreas

R1236188Lcs-50 50 ug
EUR 421.2

Mouse Pancreas PrimaCell2: Normal Pancreatic Islets

2-82081 1 Kit Ask for price

Human (Pancreas) Amylase alpha Enzyme

abx070003-100Units 100 Units
EUR 543.6

Total RNA - Diabetic Disease: Pancreas

R1236188Dia-50 50 ug
EUR 421.2

Pancreas intraepithelial neoplasia(PanIN)

BIC14011b each
EUR 222
Description: Pancreas intraepithelial neoplasia(PanIN), pancreatitis and cancer tissue array, including pathology grade, TNM and clinical stage, 24 cases/48 cores, replacing BIC14011a

MicroRNA scramble in CD511 - Virus

PMIRH000VA-1 1x10^6 IFUs
EUR 715

Pancreatic adenocarcinoma with pancreas tissue array

PA484a each
EUR 168
Description: Pancreatic adenocarcinoma with pancreas tissue array, including pathology grade, TNM and clinical stage, 24 cases/48 cores (core size2.0mm), replacing PA484

Pancreatic adenocarcinoma with pancreas tissue array

PA1001c each
EUR 306
Description: Pancreatic adenocarcinoma with pancreas tissue array, including pathology grade, TNM and clinical stage (reference AJCC 8th version), 50 cases/100 cores, replacing PA1001b

Ribonuclease A from bovine pancreas

abx082524-25mg 25 mg
EUR 226.8

Frozen Tissue Section - Lupus: Pancreas

T1236188Lup 5 slides
EUR 515

Pancreatic cancer with normal pancreas tissue array

PA961f each
EUR 306
Description: Pancreatic cancer with normal pancreas tissue array, including pathology grade, TNM and clinical stage, 96 cases/96 cores, replacing PA961e

Total Protein - Liver Cirrhosis: Pancreas

P1236188Lcs 1 mg
EUR 471

Paraffin Tissue Section - Lupus: Pancreas

T2236188Lup 5 slides
EUR 243

Total RNA - Rat Normal Tissue: Pancreas

R1434188-50 50 ug
EUR 313

Rat Pancreas PrimaCell 2: Pancreatic Epithelial Cells

2-82597 1 Kit Ask for price

Pancreas Duodenum Homeobox-1 Antibody

20-abx137204
  • EUR 543.60
  • EUR 276.00
  • EUR 393.60
  • 100 ul
  • 10 ul
  • 50 ul

Salmon pancreas disease virus PCR kit

PCR-V263-48R 50T
EUR 987.6

Salmon pancreas disease virus PCR kit

PCR-V263-96R 100T
EUR 1335.6

SanPrep Column microRNA Miniprep Kit

SK8811 50prep
EUR 151.32

Bovine pancreas Insulin (~30 USP U/mg)

INSL15-N-10 10 mg
EUR 489.6

Mouse Pancreas, Peptide Aptamer, unlabeled

AP-322-U 5 mg Ask for price

Phospholipase A2, Pancreas (pPLA2) Antibody

20-abx174050
  • EUR 1178.40
  • EUR 594.00
  • 1 mg
  • 200 ug

Phospholipase A2, Pancreas (pPLA2) Antibody

20-abx178008
  • EUR 1696.80
  • EUR 794.40
  • 1 mg
  • 200 ug

Phospholipase A2, Pancreas (pPLA2) Antibody

20-abx128460
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Pancreas PrimaCell 2: Pancreatic Epithelial Cells

2-82102 1 Kit Ask for price

Human Pancreas PrimaCell 2: Pancreatic Epithelial Cells

2-96123 1 Kit Ask for price

Total RNA - Mouse Normal Tissue: Pancreas

R1334188-50 50 ug
EUR 369

Membrane Protein - Liver Cirrhosis: Pancreas

P3236188Lcs 0.1 mg
EUR 410

Pancreas Membrane Diabetic Disease Lysate

XBL-10785 0.1 mg
EUR 752.1
Description: Human pancreas tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

cDNA - Human Adult Normal Tissue: Pancreas

C1234188 40 reactions
EUR 385

Membrane Protein - Diabetic Disease: Pancreas

P3236188Dia 0.1 mg
EUR 410

Dog Pancreastatin ELISA kit

E08P0605-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Pancreastatin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Pancreastatin ELISA kit

E08P0605-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Pancreastatin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Pancreastatin ELISA kit

E08P0605-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Pancreastatin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pancreas Dissociation System 8 (Duct), Rat

4-20378 ea Ask for price

Mouse Pancreas, Peptide Aptamer, Biotinylated

AP-322-B 1 mg Ask for price

Recombinant Phospholipase A2, Pancreas (pPLA2)

4-RPD826Hu01
  • EUR 539.33
  • EUR 267.60
  • EUR 1692.48
  • EUR 644.16
  • EUR 1168.32
  • EUR 436.80
  • EUR 4051.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Phospholipase A2, Pancreas expressed in: E.coli

cDNA - Monkey (Rhesus) Normal Tissue: Pancreas

C1534188 40 reactions
EUR 385

Total Protein - Human Tumor Tissue: Pancreas

P1235188 1 mg
EUR 471

Salmon pancreas disease virus RT PCR kit

RTq-V263-100R 100T
EUR 1573.2

Salmon pancreas disease virus RT PCR kit

RTq-V263-150R 150T
EUR 2144.4

Salmon pancreas disease virus RT PCR kit

RTq-V263-50R 50T
EUR 1155.6

Pancreas Dissociation System 3 (Duct), Mouse

4-20373 ea Ask for price

Deoxyribonuclease (1000 U/g), Bovine Pancreas

DNS-01 1 g
EUR 781.2

Total Protein - Mouse Normal Tissue: Pancreas

P1334188 1 mg
EUR 188

Frozen Tissue Section - Human Tumor: Pancreas

T1235188 5 slides
EUR 330

MicroRNA Precursor Pooled Plasmid Library

PMIRHPLPA-1 1 aliquot plasmid DNA
EUR 60

Pig Phospholipase A2, Pancreas (pPLA2) Protein

20-abx654732
  • EUR 777.60
  • EUR 326.40
  • EUR 2397.60
  • EUR 927.60
  • EUR 560.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

rno-miRNome MicroRNA Profiling Kit [Rat]

RA680B-1 20 complete profiles
EUR 2006.4

Mouse Pancreas, Peptide Aptamer, FITC labelled

AP-322-F 1 mg Ask for price

cDNA - Human Diabetic Diseased Tissue: Pancreas

C1236188Dia 40 reactions
EUR 729

Membrane Protein - Human Tumor Tissue: Pancreas

P3235188 0.1 mg
EUR 668

Paraffin Tissue Section - Human Pancreas Tumor

T2235188 5 slides
EUR 238

Rat Pancreas PrimaCell: Normal Pancreatic Islets Growth Medium

9-25078 5 x 100 ml Ask for price

cDNA - Monkey (Cynomolgus) Normal Tissue: Pancreas

C1534188-Cy 40 reactions
EUR 385

Human Phospholipase A2, Pancreas (pPLA2) Protein

20-abx166479
  • EUR 760.80
  • EUR 326.40
  • EUR 2281.20
  • EUR 895.20
  • EUR 543.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Monkey (Cynomolgus) cDNA Normal Tissue: Pancreas

MC34-188 10 rxn
EUR 498

hsa-miRNome MicroRNA Profiling Kit [Human]

RA660A-1 20 profiles
EUR 2212.8

mmu-miRNome MicroRNA Profiling Kit [Mouse]

RA670A-1 20 profiles
EUR 2046

Mouse Pancreas PrimaCell2: Normal Pancreatic Islets Growth Medium

9-32081 5 x 100 ml Ask for price

Rat Pancreas Tissue Preparation Buffer: Normal Pancreatic Islets

9-80285 1 x 100 ml Ask for price

Cancer of the pancreas, 24 cases (2mm), set 1

PAC481 1
EUR 300
Description: Pancreatic cancer tissue array, set 1, contains 20 cases of pancreatic cancers and 4 cases of normal and non-malignant pancreatic tissues in duplicates with grading and TNM staging data.

Cancer of the pancreas, 24 cases (2mm), set 2

PAC482 1
EUR 300
Description: Pancreatic cancer tissue array, set 2, contains 20 cases of pancreatic cancers and 4 cases of normal pancreatic tissues in duplicates, non-overlapping with PAC481, with grading and TNM staging data.

Pig Phospholipase A2, Pancreas (pPLA2) CLIA Kit

20-abx494402
  • EUR 10282.80
  • EUR 5472.00
  • EUR 1262.40
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

FFPE Genomic DNA - Human Tumor Tissue: Pancreas

D2235188 2 ug
EUR 960

Total RNA - Human Adult Normal Tissue: Pancreas

R1234188-50 50 ug
EUR 170

Multiple Species Tissue Slides - Pancreas Tissue

11-100-MSTA 1 pack
EUR 241.8
Description: The Multiple Species Tissue Array (MSTA) slides were designed to study protein expression patterns in different cells and tissues from multiple species. Tissue slices from three different species are mounted on each MSTA slide which can then be treated as a single histological slide for H&E staining, immunohistochemistry, or in situ hybridization. This format allows a rapid analysis of protein expression and localization across different species. MSTA slides can also be used to quickly determine the species reactivity of a given antibody.

Rat Pancreas OptiTDS: Tissue Dissociation System

4-28285 1 Kit Ask for price

Pig Phospholipase A2, Pancreas (pPLA2) ELISA Kit

20-abx154993
  • EUR 9493.20
  • EUR 5058.00
  • EUR 1167.60
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Pig Phospholipase A2, Pancreas (pPLA2) ELISA Kit

SED826Po-10x96wellstestplate 10x96-wells test plate
EUR 6777.36
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Phospholipase A2, Pancreas (pPLA2) in serum, plasma, tissue homogenates and other biological fluids.

Pig Phospholipase A2, Pancreas (pPLA2) ELISA Kit

SED826Po-1x48wellstestplate 1x48-wells test plate
EUR 663.31
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Phospholipase A2, Pancreas (pPLA2) in serum, plasma, tissue homogenates and other biological fluids.

Pig Phospholipase A2, Pancreas (pPLA2) ELISA Kit

SED826Po-1x96wellstestplate 1x96-wells test plate
EUR 896.16
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Phospholipase A2, Pancreas (pPLA2) in serum, plasma, tissue homogenates and other biological fluids.

Pig Phospholipase A2, Pancreas (pPLA2) ELISA Kit

SED826Po-5x96wellstestplate 5x96-wells test plate
EUR 3672.72
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Phospholipase A2, Pancreas (pPLA2) in serum, plasma, tissue homogenates and other biological fluids.

Pig Phospholipase A2, Pancreas (pPLA2) ELISA Kit

4-SED826Po
  • EUR 6837.60
  • EUR 3613.20
  • EUR 896.40
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Pig Phospholipase A2, Pancreas (pPLA2) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.

Rat Phospholipase A2(Pancreas(pPLA2)ELISA kit

QY-E10541 96T
EUR 480

Paraffin Tissue Section - Liver Cirrhosis: Pancreas

T2236188Lcs 5 slides
EUR 238

Cholesterol esterase (35 U/mg), Porcine Pancreas

CES-02 50000 U
EUR 6554.4

Ribonuclease (70 U/mg material), Bovine pancreas

RNU-01 500 mg
EUR 416.4

Ribonuclease (90 U/mg material), Bovine pancreas

RNU-02 500 mg
EUR 562.8

Total RNA - Monkey (Rhesus) Normal Tissue: Pancreas

R1534188-50 50 ug
EUR 191

Trypsin (5000 NF U/mg material), Porcine pancreas

TRY-01 5 g
EUR 562.8

miRZip-1 anti-miR-1 microRNA construct

MZIP1-PA-1 Bacterial Streak
EUR 620

miRZip-7 anti-miR-7 microRNA construct

MZIP7-PA-1 Bacterial Streak
EUR 620

miRZip-9 anti-miR-9 microRNA construct

MZIP9-PA-1 Bacterial Streak
EUR 620

Human Phospholipase A2, Pancreas (pPLA2)ELISA kit

201-12-2531 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Phospholipase A2, Pancreas (pPLA2) ELISA Kit

20-abx052755
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Phospholipase A2, Pancreas (pPLA2) ELISA Kit

abx570720-96tests 96 tests
EUR 943.2

Human Adult Pancreas (Normal) Whole tissue lysate

HAL-1307 1 mg
EUR 628.8

Monkey (Cyno.) Normal Pancreas Whole tissue lysate

MCL-1198 1 mg
EUR 628.8

Human Phospholipase A2, Pancreas(pPLA2)ELISA Kit

QY-E00482 96T
EUR 433.2

Mouse Phospholipase A2(Pancreas(pPLA2)ELISA kit

QY-E21174 96T
EUR 433.2

Human Phospholipase A2, Pancreas (pPLA2)ELISA kit

YLA3198HU-48T 48T
EUR 435

Human Phospholipase A2, Pancreas (pPLA2)ELISA kit

YLA3198HU-96T 96T
EUR 562.5

Mouse Pancreas OptiTDS2: Tissue Dissociation System

4-28195 1 Kit Ask for price

Total Protein - Human Adult Normal Tissue: Pancreas

P1234188 1 mg
EUR 188

Genomic DNA - Lupus: Pancreas, from a single donor

D1236188Lup 50 ug
EUR 504

Human Pancreas Tissue Slide (normal) (5 slides/pk)

HTS-11101 1 pk
EUR 343.2

OncoMir pooled microRNA precursor virus library

PMIRHOPLVA-1 1 virus aliquot
EUR 1429.2

miRZip-16 anti-miR-16 microRNA construct

MZIP16-PA-1 Bacterial Streak
EUR 620

miRZip-17 anti-miR-17 microRNA construct

MZIP17-AA-1 Miniprep DNA
EUR 620

miRZip-17 anti-miR-17 microRNA construct

MZIP17-PA-1 Bacterial Streak
EUR 620

miRZip-21 anti-miR-21 microRNA construct

MZIP21-PA-1 Bacterial Streak
EUR 620

miRZip-22 anti-miR-22 microRNA construct

MZIP22-PA-1 Bacterial Streak
EUR 620

miRZip-24 anti-miR-24 microRNA construct

MZIP24-PA-1 Bacterial Streak
EUR 620

miRZip-25 anti-miR-25 microRNA construct

MZIP25-PA-1 Bacterial Streak
EUR 620

miRZip-31 anti-miR-31 microRNA construct

MZIP31-PA-1 Bacterial Streak
EUR 620

miRZip-32 anti-miR-32 microRNA construct

MZIP32-PA-1 Bacterial Streak
EUR 620

miRZip-93 anti-miR-93 microRNA construct

MZIP93-PA-1 Bacterial Streak
EUR 620
Two ABEmax or mini ABEmax variants (TadA* fused with Cas9n) with deletion of R153 inside TadA and/or TadA* (named as del153/del153* and mini del153) are efficiently engineered, displaying minimized RNA off-targeting, however comparable DNA on-targeting actions. Furthermore, R153 deletion in lately reported ABE8e or ABE8s may also largely scale back their RNA off-targeting actions. Taken collectively, we develop a method to generate engineered ABEs (eABEs) with minimized RNA off-targeting actions.

Leave a Reply

Your email address will not be published. Required fields are marked *

Recent Posts

Categories