NMR solution structures of Runella slithyformis RNA 2′-phosphotransferase Tpt1 provide insights into NAD+ binding and specificity

NMR solution structures of Runella slithyformis RNA 2'-phosphotransferase Tpt1 provide insights into NAD+ binding and specificity

Tpt1, an integral part of the fungal and plant tRNA splicing equipment, catalyzes switch of an inside RNA 2′-PO4 to NAD+ yielding RNA 2′-OH and ADP-ribose-1′,2′-cyclic phosphate merchandise. Right here, we report NMR buildings of the Tpt1 ortholog from the bacterium Runella slithyformis (RslTpt1), as apoenzyme and certain to NAD+. RslTpt1 consists of N- and C-terminal lobes with substantial inter-lobe dynamics within the free and NAD+-bound states. ITC measurements of RslTpt1 binding to NAD+ (KD ∼31 μM), ADP-ribose (∼96 μM) and ADP (∼123 μM) point out that substrate affinity is decided primarily by the ADP moiety; no binding of NMN or nicotinamide is noticed by ITC.

NAD+-induced chemical shift perturbations (CSPs) localize completely to the RslTpt1 C-lobe. NADP+, which accommodates an adenylate 2′-PO4 (mimicking the substrate RNA 2′-PO4), binds with decrease affinity (KD ∼1 mM) and elicits solely N-lobe CSPs. The RslTpt1·NAD+ binary advanced reveals C-lobe contacts to adenosine ribose hydroxyls (His99, Thr101), the adenine nucleobase (Asn105, Asp112, Gly113, Met117) and the nicotinamide riboside (Ser125, Gln126, Asn163, Val165), a number of of that are important for RslTpt1 exercise in vivo. Proximity of the NAD+ β-phosphate to ribose-C1″ means that it could stabilize an oxocarbenium transition-state throughout step one of the Tpt1-catalyzed response.

Reverse roles of transcription elongation components Spt4/5 and Elf1 in RNA polymerase II transcription by way of B-form versus non-B DNA buildings

Transcription elongation may be affected by quite a few forms of obstacles, resembling nucleosome, pausing sequences, DNA lesions and non-B-form DNA buildings. Spt4/5 and Elf1 are conserved transcription elongation components that promote RNA polymerase II (Pol II) bypass of nucleosome and pausing sequences. Importantly, genetic research have proven that Spt4/5 performs important roles within the transcription of expanded nucleotide repeat genes related to inherited neurological illnesses. Right here, we examine the operate of Spt4/5 and Elf1 within the transcription elongation of CTG•CAG repeat utilizing an in vitro reconstituted yeast transcription system.
We discovered that Spt4/5 helps Pol II transcribe by way of the CTG•CAG tract duplex DNA, which is in good settlement with its canonical roles in stimulating transcription elongation. In sharp distinction, surprisingly, we revealed that Spt4/5 enormously inhibits Pol II transcriptional bypass of CTG and CAG slip-out buildings. Moreover, we demonstrated that transcription elongation issue Elf1 individually and cooperatively with Spt4/5 inhibits Pol II bypass of the slip-out buildings. This research uncovers the vital purposeful interplays between template DNA buildings and the operate of transcription elongation components. This research additionally expands our understanding of the capabilities of Spt4/5 and Elf1 in transcriptional processing of trinucleotide repeat DNA.

Scoring Capabilities for Protein-RNA Advanced Construction Prediction: Advances, Functions, and Future Instructions

Protein-RNA interplay is among the many most important of organic occasions in dwelling cells, being concerned in protein synthesizing, RNA processing and transport, DNA transcription, and regulation of gene expression, and lots of different important bio-molecular actions. A radical understanding of this interplay is of paramount significance in elementary research of a wide range of important mobile processes and therapeutic utility for treatment of a broad vary of illnesses. Experimental high-resolution 3D construction willpower is the first supply of information for protein-RNA complexes.
Nevertheless, attributable to technical limitations, the prevailing methods for experimental construction willpower could not match the demand from quick rising curiosity in academia and business. This downside necessitates the choice high-throughput computational technique for protein-RNA advanced construction prediction. Just like the in silico strategies used for protein-protein and protein-DNA interactions, a dependable prediction of protein-RNA advanced construction requires a scoring operate with commensurate discriminatory energy.
Derived from decided buildings and purposed to foretell the to-be-determined buildings, the scoring operate shouldn’t be solely a predictive instrument but in addition a gauge of our data of protein-RNA interplay. On this overview, we current an outline of the standing of current scoring capabilities and the scientific precept behind their constructions in addition to their strengths and limitations. Lastly, we’ll talk about about future instructions of the scoring operate growth for protein-RNA construction prediction.

Meeting and Cryo-EM construction willpower of yeast mitochondrial RNA polymerase initiation advanced intermediates

In yeast mitochondria, transcription initiation requires meeting of mitochondrial RNA polymerase and transcription initiation issue MTF1 on the DNA promoter initiation web site. This protocol describes the purification of the part proteins and meeting of partially melted and totally melted initiation advanced states. Each states co-exist in equilibrium in the identical pattern as seen by cryoelectron microscopy (cryo-EM) and permit elucidation of MTF1’s structural roles in controlling the transition into elongation. We additional define how evaluation of the advanced by mild scattering, thermal shift assay, and ultrafiltration assay reveals reproducible outcomes. For full particulars on the use and execution of this protocol, please discuss with De Wijngaert et al. (2021).
NMR solution structures of Runella slithyformis RNA 2'-phosphotransferase Tpt1 provide insights into NAD+ binding and specificity

Construction-guided engineering of adenine base editor with minimized RNA off-targeting exercise

Each adenine base editors (ABEs) and cytosine base editors (CBEs) have been lately revealed to induce transcriptome-wide RNA off-target enhancing in a information RNA-independent method. Right here we assemble a reporter system containing E.coli Hokb gene with a tRNA-like motif for strong detection of RNA enhancing actions because the optimized ABE, ABEmax, induces extremely environment friendly A-to-I (inosine) enhancing inside an E.coli tRNA-like construction. Then, we design mutations to disrupt the potential interplay between TadA and tRNAs in structure-guided ideas and discover that Arginine 153 (R153) inside TadA is important for deaminating RNAs with core tRNA-like buildings.

Dog Pancreas Specific Transcription Factor 1a ELISA kit

E08P0154-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pancreas tumor with pancreas tissue array

T143b each
EUR 66
Description: Pancreas tumor with pancreas tissue array, including TNM and clinical stage, 6 cases/24 cores, replacing T143a

Pancreas tissue array with pancreas cancer tissue

PAN241a each
EUR 198
Description: Pancreas tissue array with pancreas cancer tissue, including TNM, clinical stage and pathology grade, 24 cases/24 cores, replacing PAN241

Pancreas tissue array with pancreas cancer tissue

PAN801 each
EUR 258
Description: Pancreas tissue array with pancreas cancer tissue, including TNM, clinical stage and pathology grade, 80 cases/80 cores, replaced by PAN801a

Multiple tumor of pancreas and pancreas tissue array

T142c each
EUR 66
Description: Multiple tumor of pancreas and pancreas tissue array, including pathology grade, TNM and clinical stage, 12 cases/24 cores, replacing T142b

Pancreas Lysate

1414 0.1 mg
EUR 229.2
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Pancreas Lysate

1469 0.1 mg
EUR 229.2
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

cDNA - Lupus: Pancreas

C1236188Lup 40 reactions
EUR 811

Pancreas disease spectrum (pancreatic cancer progression) with pancreas tissue array

PA2081c each
EUR 594
Description: Pancreas disease spectrum (pancreatic cancer progression) with pancreas tissue array, including pathology grade, TNM and clinical stage, 96 cases/192 cores, replacing PA2081b

pancreas Tissue block

16 1 unit
EUR 635

Pancreas Tumor Lysate

1334 0.1 mg
EUR 304.8
Description: Pancreas tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Pancreas Lupus Lysate

XBL-10373 0.1 mg
EUR 796.2
Description: Human pancreas tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human pancreas tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the pancreas tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The pancreas tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Trypsin (human pancreas)

GWB-2BA469 0.1 mg Ask for price

Trypsin, Human Pancreas

7292-50 each
EUR 373.2

Trypsin, Human Pancreas

TRYP15-N-50 50 ug
EUR 270

OORA00343-10U - PANCREAS

OORA00343-10U 1Each
EUR 185

OORA00343-1EA - PANCREAS

OORA00343-1EA 1Each
EUR 179

OORA00484-10U - PANCREAS

OORA00484-10U 1Each
EUR 55

OORA00484-1EA - PANCREAS

OORA00484-1EA 1Each
EUR 59

Pancreas Membrane Lysate

XBL-10780 0.1 mg
EUR 619.8
Description: Human pancreas tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Chicken Pancreas Sections

CP-313 10 slides
EUR 240

Elastase, Porcine Pancreas

P1276-250 each
EUR 261.6

Human pancreas fibroblasts

P0018025 One Frozen vial
EUR 580

Chymotrypsin, Human Pancreas

CHYT15-N-100 100 ug
EUR 343.2

Chymotrypsin, Human Pancreas

7538-100 each
EUR 438

Human Pancreas Chymotrypsin

GWB-8B63CA 0.1 mg Ask for price

Pancreas Cytoplasmic Lysate

XBL-10779 0.1 mg
EUR 273.3
Description: Human pancreas tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

Cat Pancreas Genomic DNA

FG-313 0.1mg
EUR 210

Rat Pancreas Genomic DNA

RG-313 0.1mg
EUR 177

Total RNA - Lupus: Pancreas

R1236188Lup-50 50 ug
EUR 460

Pig Pancreas Genomic DNA

PG-313 0.1mg
EUR 177

Cat Pancreas Total Protein

FT-313 1mg
EUR 176

Rat Pancreas Total Protein

RT-313 1mg
EUR 153

Human Pancreas Genomic DNA

HG-313 0.05mg
EUR 210

Rat Pancreas Tissue Lysate

IRTPCSTL100UG each
EUR 245
Description: Rat Pancreas Tissue Lysate

Pig Pancreas Total Protein

PT-313 1mg
EUR 153

Mouse Pancreas Genomic DNA

MG-313 0.1mg
EUR 177

Sheep Pancreas Genomic DNA

SG-313 0.1mg
EUR 177

43031-2 CK PANCREAS 25 EA*

43031-2 25 EA
EUR 417

Bovine Pancreas Genomic DNA

BG-313 0.1mg
EUR 177

Equine Pancreas Genomic DNA

GE-313 0.1mg
EUR 210

Rabbit Pancreas Genomic DNA

TG-313 0.1mg
EUR 177

Human Pancreas Tumor lysate

HTL-1334 1 mg
EUR 927.6

OORA00540-1U - Rat PANCREAS

OORA00540-1U 1Each
EUR 60

Trypsin from bovin pancreas

TN6872-10mg 10mg Ask for price
Description: Trypsin from bovin pancreas

Trypsin from bovin pancreas

TN6872-1g 1g Ask for price
Description: Trypsin from bovin pancreas

Trypsin from bovin pancreas

TN6872-1mg 1mg Ask for price
Description: Trypsin from bovin pancreas

Trypsin from bovin pancreas

TN6872-50mg 50mg Ask for price
Description: Trypsin from bovin pancreas

Trypsin from bovin pancreas

TN6872-5mg 5mg Ask for price
Description: Trypsin from bovin pancreas

Pancreas Tissue Lysate (Tumor)

1737-01 0.1 mg
EUR 336.3
Description: Pancreas tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Pancreas Tissue Lysate (Tumor)

1737-02 0.1 mg
EUR 336.3
Description: Pancreas tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Pancreas Tissue Lysate (Tumor)

1738-01 0.1 mg
EUR 336.3
Description: Pancreas tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Pancreas Tissue Slide (Normal)

11-101-10um 10 um
EUR 241.8

Pancreas Tissue Slide (Normal)

11-101-4um 4 um
EUR 216.6

Lipase from Porcine Pancreas

20552-02 25G
EUR 26.25

Human Pancreas Tissue Lysate

30R-AP030 150 ug
EUR 210
Description: Fresh tissue lysate isolated from human pancreas

Hamster Pancreas Genomic DNA

GA-313 0.1mg
EUR 177

Cat Pancreas Frozen Sections

FF-313 10 slides
EUR 261

Chicken Pancreas Genomic DNA

GC-313 0.1mg
EUR 177

Insulin from bovine pancreas

abx082234-25mg 25 mg
EUR 260.4

Rat Pancreas Frozen Sections

RF-313 10 slides
EUR 228

Sheep Pancreas Total Protein

ST-313 1mg
EUR 153

Mouse Pancreas Tissue Lysate

IMSPCSTL100UG each
EUR 245
Description: Mouse Pancreas Tissue Lysate

Human Pancreas Total Protein

HT-313 1mg
EUR 176

Human Pancreas Amylase alpha

GWB-759CBC 100 Units Ask for price

OORA00540-1EA - Rat PANCREAS

OORA00540-1EA 1Each
EUR 59

Pig Pancreas Frozen Sections

PF-313 10 slides
EUR 261

Total Protein - Lupus: Pancreas

P1236188Lup 1 mg
EUR 559

Insulin from bovine pancreas

abx082234-100l 100 µl
EUR 212.5

Insulin from bovine pancreas

abx082234-1ml 1 ml Ask for price

Insulin from bovine pancreas

abx082234-200l 200 µl Ask for price

Pancreas Tissue Lysate (Normal)

1736-02 0.1 mg
EUR 260.7
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Pancreas Tissue Lysate (Normal)

1736-05 0.1 mg
EUR 260.7
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Trypsin from Porcine Pancreas

35547-51 1G
EUR 19.25

Trypsin from Porcine Pancreas

35547-64 10G
EUR 92.4

Bovine Pancreas Total Protein

BT-313 1mg
EUR 153

Autopsy pancreas Tissue block

79 1 unit
EUR 500

cDNA - Liver Cirrhosis: Pancreas

C1236188Lcs 40 reactions
EUR 802

Human Pancreas Acetone Powder

IRHUPCSP1GM each
EUR 422
Description: Human Pancreas Acetone Powder

Salmon pancreas disease virus

Oneq-V263-OneqV263100R Oneq-V263-100R
EUR 1028

Salmon pancreas disease virus

Oneq-V263-OneqV263150R Oneq-V263-150R
EUR 1438

Salmon pancreas disease virus

Oneq-V263-OneqV26350R Oneq-V263-50R
EUR 728

Salmon pancreas disease virus

PCR-V263-PCRV26348R PCR-V263-48R
EUR 485

Salmon pancreas disease virus

PCR-V263-PCRV26396R PCR-V263-96R
EUR 685

Rabbit Pancreas Total Protein

TT-313 1mg
EUR 153

Pancreas Tissue Slide (Abnormal)

11-106-10um 10 um
EUR 241.8

Pancreas Tissue Slide (Abnormal)

11-106-4um 4 um
EUR 216.6

Elastase from Porcine Pancreas

14363-14 10MG
EUR 76.3

Elastase from Porcine Pancreas

14363-56 50MG
EUR 245

Elastase from Porcine Pancreas

30047 1 mg
EUR 42
Description: Elastase from Porcine Pancreas

Chicken Pancreas Total Protein

CT-313 1mg
EUR 140

Cat Pancreas Paraffin Sections

FP-313 10 slides
EUR 240

Hamster Pancreas Total Protein

AT-313 1mg
EUR 153

Rat Pancreas Paraffin Sections

RP-313 10 slides
EUR 228

Elastase from porcine pancreas

T40495-10mg 10mg Ask for price
Description: Elastase from porcine pancreas

Elastase from porcine pancreas

T40495-1g 1g Ask for price
Description: Elastase from porcine pancreas

Elastase from porcine pancreas

T40495-1mg 1mg Ask for price
Description: Elastase from porcine pancreas

Elastase from porcine pancreas

T40495-50mg 50mg Ask for price
Description: Elastase from porcine pancreas

Elastase from porcine pancreas

T40495-5mg 5mg Ask for price
Description: Elastase from porcine pancreas

Human Pancreas Frozen Sections

HF-313 10slides
EUR 240

OORA00511-1U - Rabbit PANCREAS

OORA00511-1U 1Each
EUR 255

MiniPig Pancreas Total Protein

NT-313 1mg
EUR 176

Pig Pancreas Paraffin Sections

PP-313 10 slides
EUR 240

Alpha-Amylase, Human Pancreas

P1454-100 each
EUR 444

Alpha-Amylase, Human Pancreas

P1545-100 100 units
EUR 417.6

Sheep Pancreas Frozen Sections

SF-313 10 slides
EUR 261

Pancreas Membrane Tumor Lysate

XBL-10795 0.1 mg
EUR 1023
Description: Human pancreas tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

CK PANCREAS POWDER LYOPH 1g*

43032-0 1g
EUR 147.17

59431-2 PIG PANCREAS 10 EA*

59431-2 10 EA
EUR 275

Bovine Pancreas Frozen Sections

BF-313 10 slides
EUR 261

Rabbit Pancreas Frozen Sections

TF-313 10 slides
EUR 240

OORA00511-1EA - Rabbit PANCREAS

OORA00511-1EA 1Each
EUR 239

Pancreas Liver Cirrhosis Lysate

XBL-10372 0.1 mg
EUR 796.2
Description: Human pancreas tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human pancreas tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the pancreas tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The pancreas tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Trypsin, Porcine Pancreas, ≥2500 USP U/MG

A4029-1G 1G
EUR 49.5
Description: Biotechnology

Trypsin, Porcine Pancreas, ≥2500 USP U/MG

A4029-5G 5G
EUR 198
Description: Biotechnology

41231-2 RB PANCREAS Y 25 EA*

41231-2 25 EA
EUR 287

Hamster Pancreas Frozen Sections

AF-313 10 slides
EUR 240

Chicken Pancreas Frozen Sections

CF-313 10 slides
EUR 261

Sheep Pancreas Paraffin Sections

SP-313 10 slides
EUR 240

Human Pancreas Paraffin Sections

HP-313 10 slides
EUR 228

MiniPig Pancreas Frozen Sections

NF-313 10 slides
EUR 307

OORA00458-10U - Hamster PANCREAS

OORA00458-10U 1Each
EUR 75

OORA00458-1EA - Hamster PANCREAS

OORA00458-1EA 1Each
EUR 69

Mini Pig Pancreas Genomic DNA

GN-313 0.1mg
EUR 210

Rat WS Pancreas Total Protein

RT-313-WS 1mg
EUR 153

Cancer of the pancreas, 102 cases (1.5mm)

PAC1021 1
EUR 354
Description: Pancreatic cancer tissue array contains 96 cases of pancreatic cancers and 6 cases of normal and non-malignant pancreatic tissues with grading and TNM staging data.

Pancreatic cancer with pancreas tissue array

PA2082a each
EUR 546
Description: Pancreatic cancer with pancreas tissue array, including ductal adenocarcinoma(PADC),acinar cell carcinoma(PACC),normal adjacent and pancreas tissue,pathology grade, TNM and clinical stage, 96 cases/192 cores(1.0mm), replacing PA2082

Pancreatic cancer with pancreas tissue array

PA805c each
EUR 306
Description: Pancreatic cancer with pancreas tissue array, including duct adenocarcinoma(PDAC), acinar cell carcinoma(PACC),squamous cell carcinoma,normal adjacent pancreas and normal tissuepathology grade, TNM and clinical stage, 80 cases/80 cores(1.5mm), replacing PA805b

Pancreatic cancer with pancreas tissue array

T141c each
EUR 48
Description: Pancreatic cancer with pancreas tissue array, including pathology grade, TNM and clinical stage, 6 cases/24 cores, replacing T141b

Bovine Pancreas Paraffin Sections

BP-313 10 slides
EUR 240

OCPB00058-100UG - Pancreas Lysate

OCPB00058-100UG 0.1mg
EUR 139

OCPB00073-100UG - Pancreas Lysate

OCPB00073-100UG 0.1mg
EUR 139

Fetal Pancreas Cytoplasmic Lysate

XBL-10791 0.1 mg
EUR 273.3
Description: Human pancreas tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The fetal human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

Pancreas Cytoplasmic Tumor Lysate

XBL-10794 0.1 mg
EUR 273.3
Description: Human pancreas tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

Trypsin 1:250, Porcine Pancreas

A4027-100G 100G
EUR 97.9
Description: Biotechnology

Trypsin 1:250, Porcine Pancreas

A4027-25G 25G
EUR 28.6
Description: Biotechnology

Trypsin 1:250, Porcine Pancreas

A4027-500G 500G
EUR 200.2
Description: Biotechnology

Trypsin 1:300, Porcine Pancreas

A4028-25G 25G
EUR 66
Description: Biotechnology

Rat Pancreas Whole Cell Lysate

IRTPCSTLW500UG each
EUR 327
Description: Rat Pancreas Whole Cell Lysate

Mouse C57 Pancreas Genomic DNA

MG-313-C57 0.1mg
EUR 210

Hamster Pancreas Paraffin Sections

AP-313 10 slides
EUR 228

MiniPig Pancreas Paraffin Sections

NP-313 10 slides
EUR 307

Guinea Pig Pancreas Genomic DNA

GG-313 0.1mg
EUR 177

Rat WS Pancreas Frozen Sections

RF-313-WS 10 slides
EUR 228

Human Pancreas Chymotrypsin Protein

abx060937-100ug 100 ug
EUR 577.2

Rat Pancreas PrimaCell: Normal Pancreatic Islets

2-82578 1 Kit Ask for price

cDNA - Human Tumor Tissue: Pancreas

C1235188-10 10 reactions
EUR 301

Rat Pancreas Whole tissue lysate

RAL-1469 1 mg
EUR 628.8

Mouse CD1 Pancreas Total Protein

MT-313 1mg
EUR 153

Mouse BLC Pancreas Total Protein

MT-313-BLC 1mg
EUR 180

Mouse C57 Pancreas Total Protein

MT-313-C57 1mg
EUR 180

DNase I, Bovine Pancreas, >2000 Kunitz U/MG

A4193-100MG 100MG
EUR 99
Description: Biotechnology

DNase I, Bovine Pancreas, >2000 Kunitz U/MG

A4193-1G 1G
EUR 539
Description: Biotechnology

DNase I, Bovine Pancreas, >2000 Kunitz U/MG

A4193-500MG 500MG
EUR 301.4
Description: Biotechnology

Pancreas Tissue Slide (Adenocarcinoma)

11-105-10um 10 um
EUR 241.8

Pancreas Tissue Slide (Adenocarcinoma)

11-105-4um 4 um
EUR 216.6

41331-2 RB PANCREAS MATURE 25 EA*

41331-2 25 EA
EUR 287

DNase II, Porcine Pancreas, >12000 Kunitz U/MG

A2444-20KU 20KU
EUR 462
Description: Biotechnology

Guinea Pig Pancreas Total Protein

GT-313 1mg
EUR 153

Pig (Pancreas) alpha Amylase Enzyme

abx070001-25kU 25 kU
EUR 777.6

Rat WS Pancreas Paraffin Sections

RP-313-WS 10 slides
EUR 228

Mouse Pancreas PrimaCell2: Normal Pancreatic Islets

2-82081 1 Kit Ask for price

Mouse Pancreas Whole tissue lysate

MAL-1414 1 mg
EUR 628.8

Human Lupus Pancreas Tissue Lysate

IHUPCSLUPPTL100UG each
EUR 1413
Description: Human Lupus Pancreas Tissue Lysate

Human Pancreas Tumor Tissue Lysate

IHUPCSTLT100UG each
EUR 361
Description: Human Pancreas Tumor Tissue Lysate

Total RNA - Liver Cirrhosis: Pancreas

R1236188Lcs-50 50 ug
EUR 460
Two ABEmax or mini ABEmax variants (TadA* fused with Cas9n) with deletion of R153 inside TadA and/or TadA* (named as del153/del153* and mini del153) are efficiently engineered, displaying minimized RNA off-targeting, however comparable DNA on-targeting actions. Furthermore, R153 deletion in lately reported ABE8e or ABE8s may also largely scale back their RNA off-targeting actions. Taken collectively, we develop a method to generate engineered ABEs (eABEs) with minimized RNA off-targeting actions.

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