NMR solution structures of Runella slithyformis RNA 2′-phosphotransferase Tpt1 provide insights into NAD+ binding and specificity

NMR solution structures of Runella slithyformis RNA 2'-phosphotransferase Tpt1 provide insights into NAD+ binding and specificity

Tpt1, an integral part of the fungal and plant tRNA splicing equipment, catalyzes switch of an inside RNA 2′-PO4 to NAD+ yielding RNA 2′-OH and ADP-ribose-1′,2′-cyclic phosphate merchandise. Right here, we report NMR buildings of the Tpt1 ortholog from the bacterium Runella slithyformis (RslTpt1), as apoenzyme and certain to NAD+. RslTpt1 consists of N- and C-terminal lobes with substantial inter-lobe dynamics within the free and NAD+-bound states. ITC measurements of RslTpt1 binding to NAD+ (KD ∼31 μM), ADP-ribose (∼96 μM) and ADP (∼123 μM) point out that substrate affinity is decided primarily by the ADP moiety; no binding of NMN or nicotinamide is noticed by ITC.

NAD+-induced chemical shift perturbations (CSPs) localize completely to the RslTpt1 C-lobe. NADP+, which accommodates an adenylate 2′-PO4 (mimicking the substrate RNA 2′-PO4), binds with decrease affinity (KD ∼1 mM) and elicits solely N-lobe CSPs. The RslTpt1·NAD+ binary advanced reveals C-lobe contacts to adenosine ribose hydroxyls (His99, Thr101), the adenine nucleobase (Asn105, Asp112, Gly113, Met117) and the nicotinamide riboside (Ser125, Gln126, Asn163, Val165), a number of of that are important for RslTpt1 exercise in vivo. Proximity of the NAD+ β-phosphate to ribose-C1″ means that it could stabilize an oxocarbenium transition-state throughout step one of the Tpt1-catalyzed response.

Reverse roles of transcription elongation components Spt4/5 and Elf1 in RNA polymerase II transcription by way of B-form versus non-B DNA buildings

Transcription elongation may be affected by quite a few forms of obstacles, resembling nucleosome, pausing sequences, DNA lesions and non-B-form DNA buildings. Spt4/5 and Elf1 are conserved transcription elongation components that promote RNA polymerase II (Pol II) bypass of nucleosome and pausing sequences. Importantly, genetic research have proven that Spt4/5 performs important roles within the transcription of expanded nucleotide repeat genes related to inherited neurological illnesses. Right here, we examine the operate of Spt4/5 and Elf1 within the transcription elongation of CTG•CAG repeat utilizing an in vitro reconstituted yeast transcription system.
We discovered that Spt4/5 helps Pol II transcribe by way of the CTG•CAG tract duplex DNA, which is in good settlement with its canonical roles in stimulating transcription elongation. In sharp distinction, surprisingly, we revealed that Spt4/5 enormously inhibits Pol II transcriptional bypass of CTG and CAG slip-out buildings. Moreover, we demonstrated that transcription elongation issue Elf1 individually and cooperatively with Spt4/5 inhibits Pol II bypass of the slip-out buildings. This research uncovers the vital purposeful interplays between template DNA buildings and the operate of transcription elongation components. This research additionally expands our understanding of the capabilities of Spt4/5 and Elf1 in transcriptional processing of trinucleotide repeat DNA.

Scoring Capabilities for Protein-RNA Advanced Construction Prediction: Advances, Functions, and Future Instructions

Protein-RNA interplay is among the many most important of organic occasions in dwelling cells, being concerned in protein synthesizing, RNA processing and transport, DNA transcription, and regulation of gene expression, and lots of different important bio-molecular actions. A radical understanding of this interplay is of paramount significance in elementary research of a wide range of important mobile processes and therapeutic utility for treatment of a broad vary of illnesses. Experimental high-resolution 3D construction willpower is the first supply of information for protein-RNA complexes.
Nevertheless, attributable to technical limitations, the prevailing methods for experimental construction willpower could not match the demand from quick rising curiosity in academia and business. This downside necessitates the choice high-throughput computational technique for protein-RNA advanced construction prediction. Just like the in silico strategies used for protein-protein and protein-DNA interactions, a dependable prediction of protein-RNA advanced construction requires a scoring operate with commensurate discriminatory energy.
Derived from decided buildings and purposed to foretell the to-be-determined buildings, the scoring operate shouldn’t be solely a predictive instrument but in addition a gauge of our data of protein-RNA interplay. On this overview, we current an outline of the standing of current scoring capabilities and the scientific precept behind their constructions in addition to their strengths and limitations. Lastly, we’ll talk about about future instructions of the scoring operate growth for protein-RNA construction prediction.

Meeting and Cryo-EM construction willpower of yeast mitochondrial RNA polymerase initiation advanced intermediates

In yeast mitochondria, transcription initiation requires meeting of mitochondrial RNA polymerase and transcription initiation issue MTF1 on the DNA promoter initiation web site. This protocol describes the purification of the part proteins and meeting of partially melted and totally melted initiation advanced states. Each states co-exist in equilibrium in the identical pattern as seen by cryoelectron microscopy (cryo-EM) and permit elucidation of MTF1’s structural roles in controlling the transition into elongation. We additional define how evaluation of the advanced by mild scattering, thermal shift assay, and ultrafiltration assay reveals reproducible outcomes. For full particulars on the use and execution of this protocol, please discuss with De Wijngaert et al. (2021).
NMR solution structures of Runella slithyformis RNA 2'-phosphotransferase Tpt1 provide insights into NAD+ binding and specificity

Construction-guided engineering of adenine base editor with minimized RNA off-targeting exercise

Each adenine base editors (ABEs) and cytosine base editors (CBEs) have been lately revealed to induce transcriptome-wide RNA off-target enhancing in a information RNA-independent method. Right here we assemble a reporter system containing E.coli Hokb gene with a tRNA-like motif for strong detection of RNA enhancing actions because the optimized ABE, ABEmax, induces extremely environment friendly A-to-I (inosine) enhancing inside an E.coli tRNA-like construction. Then, we design mutations to disrupt the potential interplay between TadA and tRNAs in structure-guided ideas and discover that Arginine 153 (R153) inside TadA is important for deaminating RNAs with core tRNA-like buildings.

Dog Pancreas Specific Transcription Factor 1a ELISA kit

E08P0154-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pancreas tumor with pancreas tissue array

T143b each
EUR 66
Description: Pancreas tumor with pancreas tissue array, including TNM and clinical stage, 6 cases/24 cores, replacing T143a

Pancreas tissue array with pancreas cancer tissue

PAN241a each
EUR 198
Description: Pancreas tissue array with pancreas cancer tissue, including TNM, clinical stage and pathology grade, 24 cases/24 cores, replacing PAN241

Pancreas tissue array with pancreas cancer tissue

PAN801 each
EUR 258
Description: Pancreas tissue array with pancreas cancer tissue, including TNM, clinical stage and pathology grade, 80 cases/80 cores, replaced by PAN801a

Multiple tumor of pancreas and pancreas tissue array

T142c each
EUR 66
Description: Multiple tumor of pancreas and pancreas tissue array, including pathology grade, TNM and clinical stage, 12 cases/24 cores, replacing T142b

Pancreas Lysate

1414 0.1 mg
EUR 229.2
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Pancreas Lysate

1469 0.1 mg
EUR 229.2
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Pancreas, Rabbit

MBS639373-25Each 25Each
EUR 380

Pancreas, Rabbit

MBS639373-5x25Each 5x25Each
EUR 1480

Pancreas Lysate

MBS151609-01mg 0.1mg
EUR 200

Pancreas Lysate

MBS151609-5x01mg 5x0.1mg
EUR 880

Pancreas Lysate

MBS151660-01mg 0.1mg
EUR 200

Pancreas Lysate

MBS151660-5x01mg 5x0.1mg
EUR 880

Pancreas Lysate

MBS151662-01mg 0.1mg
EUR 200

Pancreas Lysate

MBS151662-5x01mg 5x0.1mg
EUR 880

Pancreatin, Porcine Pancreas

MBS653745-1g 1g
EUR 175

Pancreatin, Porcine Pancreas

MBS653745-25g 25g
EUR 325

Pancreatin, Porcine Pancreas

MBS653745-50g 50g
EUR 505

Pancreatin, Porcine Pancreas

MBS653745-5g 5g
EUR 225

Pancreatin, Porcine Pancreas

MBS653745-5x50g 5x50g
EUR 2125

Pancreas disease spectrum (pancreatic cancer progression) with pancreas tissue array

PA2081c each
EUR 594
Description: Pancreas disease spectrum (pancreatic cancer progression) with pancreas tissue array, including pathology grade, TNM and clinical stage, 96 cases/192 cores, replacing PA2081b

cDNA - Lupus: Pancreas

C1236188Lup 40 reactions
EUR 811

pancreas Tissue block

16 1 unit
EUR 635

Pancreas Tumor Lysate

1334 0.1 mg
EUR 304.8
Description: Pancreas tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Pancreas Lupus Lysate

XBL-10373 0.1 mg
EUR 796.2
Description: Human pancreas tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human pancreas tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the pancreas tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The pancreas tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Lipase, Human Pancreas

MBS653675-0025mg 0.025mg
EUR 530

Lipase, Human Pancreas

MBS653675-5x0025mg 5x0.025mg
EUR 2235

Lipase, Human Pancreas

MBS173153-1000Units 1000Units
EUR 1200

Lipase, Human Pancreas

MBS173153-100Units 100Units
EUR 350

Lipase, Human Pancreas

MBS173153-5x1000Units 5x1000Units
EUR 5200

Pancreas Tumor Lysate

MBS151622-01mg 0.1mg
EUR 260

Pancreas Tumor Lysate

MBS151622-5x01mg 5x0.1mg
EUR 1140

Trypsin, Human Pancreas

7292-50 each
EUR 373.2

Trypsin (human pancreas)

GWB-2BA469 0.1 mg Ask for price

Trypsin, Human Pancreas

TRYP15-N-50 50 ug
EUR 270

Trypsin, Human Pancreas

MBS135619-01mg 0.1mg
EUR 495

Trypsin, Human Pancreas

MBS135619-05mg 0.5mg
EUR 1450

Trypsin, Human Pancreas

MBS135619-5x05mg 5x0.5mg
EUR 6330

Trypsin, Human Pancreas

16-19-032000-100ug 100ug
EUR 205

Trypsin, Human Pancreas

16-19-032000-1mg 1mg
EUR 1200

Stem Cell MicroRNA Profiling Panel

RA620A-1 10 profiles
EUR 1406.4

Trypsin, Bovine Pancreas

MBS653654-1g 1g
EUR 760

Trypsin, Bovine Pancreas

MBS653654-5x1g 5x1g
EUR 3265

Pancreas Membrane Lysate

XBL-10780 0.1 mg
EUR 619.8
Description: Human pancreas tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

OORA00343-10U - PANCREAS

OORA00343-10U 1Each
EUR 185

OORA00343-1EA - PANCREAS

OORA00343-1EA 1Each
EUR 179

OORA00484-10U - PANCREAS

OORA00484-10U 1Each
EUR 55

OORA00484-1EA - PANCREAS

OORA00484-1EA 1Each
EUR 59

Insulin, Porcine Pancreas

MBS170511-10mg 10mg
EUR 245

Insulin, Porcine Pancreas

MBS170511-50mg 50mg
EUR 530

Insulin, Porcine Pancreas

MBS170511-5x50mg 5x50mg
EUR 2175

Chicken Pancreas Sections

CP-313 10 slides
EUR 240

Elastase, Porcine Pancreas

P1276-250 each
EUR 261.6

Elastase, Porcine Pancreas

MBS634645-10mg 10mg
EUR 395

Elastase, Porcine Pancreas

MBS634645-5x10mg 5x10mg
EUR 1640

Colipase, Porcine Pancreas

MBS170510-INQUIRE INQUIRE Ask for price

Elastase, Porcine Pancreas

MBS173124-1000Units 1000Units
EUR 215

Elastase, Porcine Pancreas

MBS173124-10KUnits 10KUnits
EUR 865

Elastase, Porcine Pancreas

MBS173124-25KUnits 25KUnits
EUR 1585

Elastase, Porcine Pancreas

MBS173124-5x25KUnits 5x25KUnits
EUR 6920

microRNA-21-IN-2

T61093-10mg 10mg Ask for price
Description: microRNA-21-IN-2

microRNA-21-IN-2

T61093-1g 1g Ask for price
Description: microRNA-21-IN-2

microRNA-21-IN-2

T61093-1mg 1mg Ask for price
Description: microRNA-21-IN-2

microRNA-21-IN-2

T61093-50mg 50mg Ask for price
Description: microRNA-21-IN-2

microRNA-21-IN-2

T61093-5mg 5mg Ask for price
Description: microRNA-21-IN-2

microRNA-21-IN-1

T63879-10mg 10mg Ask for price
Description: microRNA-21-IN-1

microRNA-21-IN-1

T63879-1g 1g Ask for price
Description: microRNA-21-IN-1

microRNA-21-IN-1

T63879-1mg 1mg Ask for price
Description: microRNA-21-IN-1

microRNA-21-IN-1

T63879-50mg 50mg Ask for price
Description: microRNA-21-IN-1

microRNA-21-IN-1

T63879-5mg 5mg Ask for price
Description: microRNA-21-IN-1

microRNA-21-IN-2

HY-151516 10 mg
EUR 346.33
Description: microRNA-21-IN-2 is a potential miR-21 inhibitor with an AC50 value of 3.29 μM. microRNA-21-IN-2 can be used for the research of cancer[1].

microRNA-21-IN-1

HY-146411 1 mg
EUR 2943.77
Description: microRNA-21-IN-1 (compound 7A) is an efficient microRNA inhibitor. microRNA-21-IN-1 has antiproliferative activity against Hela and HCT-116 cells with IC50s of 5.5 μM and 2.8 μM respectively, as well as promotes apoptosis of Hela cells. microRNA-21-IN-1 upregulates the expression of microRNA-21 downstream functional targets (PTEN, EGR1 and SLIT2). microRNA-21-IN-1 can be used for researching anticancer[1].

microRNA-21-IN-3

HY-153534 1 mg
EUR 2056.31
Description: microRNA-21-IN-3 (compound 45) can specifically bind to the precursor of oncogenic and pro-inflammatory microRNA-21 with medium nanomolar affinity, reduce cancer cell proliferation and miR-21 levels, and can be used in antitumor research[1].

Human pancreas fibroblasts

P0018025 One Frozen vial
EUR 580

Chymotrypsin, Human Pancreas

7538-100 each
EUR 438

Chymotrypsin, Human Pancreas

CHYT15-N-100 100 ug
EUR 343.2

Pancreas Cytoplasmic Lysate

XBL-10779 0.1 mg
EUR 273.3
Description: Human pancreas tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

Human Pancreas Chymotrypsin

GWB-8B63CA 0.1 mg Ask for price

Chymotrypsin, Human Pancreas

MBS606650-02mg 0.2mg
EUR 705

Chymotrypsin, Human Pancreas

MBS606650-5x02mg 5x0.2mg
EUR 3030

Chymotrypsin, Human Pancreas

MBS612394-1mL 1mL
EUR 575

Chymotrypsin, Human Pancreas

MBS612394-5x1mL 5x1mL
EUR 2440

Chymotrypsin, Human Pancreas

MBS655335-01mg 0.1mg
EUR 540

Chymotrypsin, Human Pancreas

MBS655335-5x01mg 5x0.1mg
EUR 2275

Chymotrypsin, Human Pancreas

MBS655348-01mg 0.1mg
EUR 665

Chymotrypsin, Human Pancreas

MBS655348-5x01mg 5x0.1mg
EUR 2850

Kallikrein, Porcine Pancreas

MBS173091-1000Units 1000Units
EUR 610

Kallikrein, Porcine Pancreas

MBS173091-5x1000Units 5x1000Units
EUR 2545

Chymotrypsin, Human Pancreas

16-19-030820-100ug 100ug
EUR 135

Chymotrypsin, Human Pancreas

16-19-030820-1mg 1mg
EUR 950

Cat Pancreas Genomic DNA

FG-313 0.1mg
EUR 210

Pig Pancreas Genomic DNA

PG-313 0.1mg
EUR 177

Total RNA - Lupus: Pancreas

R1236188Lup-50 50 ug
EUR 460

Rat Pancreas Genomic DNA

RG-313 0.1mg
EUR 177

RNase A, bovine pancreas

HY-129046 25 mg
EUR 54.11
Description: Ribonuclease A (EC 3.1.27.5) cleaves RNA 3′ to pyrimidines and actively cleaves RNA at every pyrimidine residue. Ribonuclease A catalyzes the hydrolysis of single stranded RNA in the absence of metal ions or cofactors[1][2][3].

DNase I, Bovine pancreas

HY-108882 100 mg
EUR 32.47
Description: DNase I (EC 3.1.21.1) is an enzyme that degrade DNA, it plays a key role in the cleavage of extracellular DNA is crucial for limiting the inflammatory response and maintaining homeostasis. Exogenous deoxyribonuclease shows beneficial effects in inflammatory diseases and cancer[1].

Pancreatin 4NF ex. Porcine Pancreas, 100U/mg

41421 100 Gms
EUR 21.75
Description: Part B

Dog Pancreastatin ELISA kit

E08P0605-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Pancreastatin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Pancreastatin ELISA kit

E08P0605-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Pancreastatin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Pancreastatin ELISA kit

E08P0605-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Pancreastatin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Cat Pancreas Total Protein

FT-313 1mg
EUR 176

Human Pancreas Genomic DNA

HG-313 0.05mg
EUR 210

Rat Pancreas Tissue Lysate

IRTPCSTL100UG each
EUR 245
Description: Rat Pancreas Tissue Lysate

Pig Pancreas Total Protein

PT-313 1mg
EUR 153

Mouse Pancreas Genomic DNA

MG-313 0.1mg
EUR 177

Rat Pancreas Total Protein

RT-313 1mg
EUR 153

Sheep Pancreas Genomic DNA

SG-313 0.1mg
EUR 177

Rat Pancreas Tissue Lysate

MBS8421517-01mg 0.1mg
EUR 385

Rat Pancreas Tissue Lysate

MBS8421517-5x01mg 5x0.1mg
EUR 1540

a-Amylase, Human Pancreas

MBS173014-1000Units 1000Units
EUR 1200

a-Amylase, Human Pancreas

MBS173014-5x1000Units 5x1000Units
EUR 5200

Bovine Pancreas Genomic DNA

BG-313 0.1mg
EUR 177

Human Pancreas Tumor lysate

HTL-1334 1 mg
EUR 927.6

Equine Pancreas Genomic DNA

GE-313 0.1mg
EUR 210

OORA00540-1U - Rat PANCREAS

OORA00540-1U 1Each
EUR 60

Rabbit Pancreas Genomic DNA

TG-313 0.1mg
EUR 177

Trypsin from bovin pancreas

TN6872-10mg 10mg Ask for price
Description: Trypsin from bovin pancreas

Trypsin from bovin pancreas

TN6872-1g 1g Ask for price
Description: Trypsin from bovin pancreas

Trypsin from bovin pancreas

TN6872-1mg 1mg Ask for price
Description: Trypsin from bovin pancreas

Trypsin from bovin pancreas

TN6872-50mg 50mg Ask for price
Description: Trypsin from bovin pancreas

Trypsin from bovin pancreas

TN6872-5mg 5mg Ask for price
Description: Trypsin from bovin pancreas

Trypsin from bovin pancreas

MBS5788862-INQUIRE INQUIRE Ask for price

MicroRNA Isolation Kit

KS341025 1 kit
EUR 284

Insulin from bovine pancreas

abx082234-25mg 25 mg
EUR 260.4

Pancreas Tissue Slide (Normal)

11-101-10um 10 um
EUR 241.8

Pancreas Tissue Slide (Normal)

11-101-4um 4 um
EUR 216.6

Pancreas Tissue Lysate (Tumor)

1737-01 0.1 mg
EUR 336.3
Description: Pancreas tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Pancreas Tissue Lysate (Tumor)

1737-02 0.1 mg
EUR 336.3
Description: Pancreas tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Pancreas Tissue Lysate (Tumor)

1738-01 0.1 mg
EUR 336.3
Description: Pancreas tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Lipase from Porcine Pancreas

20552-02 25G
EUR 26.25

Human Pancreas Tissue Lysate

30R-AP030 150 ug
EUR 210
Description: Fresh tissue lysate isolated from human pancreas

Hamster Pancreas Genomic DNA

GA-313 0.1mg
EUR 177

Cat Pancreas Frozen Sections

FF-313 10 slides
EUR 261

Human Pancreas Total Protein

HT-313 1mg
EUR 176

Human Pancreas Amylase alpha

GWB-759CBC 100 Units Ask for price

OORA00540-1EA - Rat PANCREAS

OORA00540-1EA 1Each
EUR 59

Pig Pancreas Frozen Sections

PF-313 10 slides
EUR 261

Mouse Pancreas Tissue Lysate

IMSPCSTL100UG each
EUR 245
Description: Mouse Pancreas Tissue Lysate

Chicken Pancreas Genomic DNA

GC-313 0.1mg
EUR 177

Total Protein - Lupus: Pancreas

P1236188Lup 1 mg
EUR 559

Rat Pancreas Frozen Sections

RF-313 10 slides
EUR 228

Sheep Pancreas Total Protein

ST-313 1mg
EUR 153

Insulin from bovine pancreas

abx082234-100l 100 µl
EUR 212.5

Insulin from bovine pancreas

abx082234-1ml 1 ml Ask for price

Insulin from bovine pancreas

abx082234-200l 200 µl Ask for price

Amylase, alpha, Human Pancreas

MBS653881-5x100Units 5x100Units
EUR 2050

Amylase, alpha, Human Pancreas

MBS653881-100Units 100Units
EUR 490

Mouse Pancreas Tissue Lysate

MBS8420796-01mg 0.1mg
EUR 385

Mouse Pancreas Tissue Lysate

MBS8420796-5x01mg 5x0.1mg
EUR 1540

Pancreas Tissue Slide (Normal)

MBS154294-10um 10um
EUR 210

Pancreas Tissue Slide (Normal)

MBS154294-4um 4um
EUR 195

Pancreas Tissue Slide (Normal)

MBS154294-5x10um 5x10um
EUR 920

Human Pancreas Tissue Lysate

MBS537637-015mg 0.15mg
EUR 380

Human Pancreas Tissue Lysate

MBS537637-5x015mg 5x0.15mg
EUR 1550

43031-2 CK PANCREAS 25 EA*

43031-2 25 EA
EUR 417

Autopsy pancreas Tissue block

79 1 unit
EUR 500

Pancreas Tissue Lysate (Normal)

1736-02 0.1 mg
EUR 260.7
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Pancreas Tissue Lysate (Normal)

1736-05 0.1 mg
EUR 260.7
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Trypsin from Porcine Pancreas

35547-51 1G
EUR 19.25

Trypsin from Porcine Pancreas

35547-64 10G
EUR 92.4

Bovine Pancreas Total Protein

BT-313 1mg
EUR 153

cDNA - Liver Cirrhosis: Pancreas

C1236188Lcs 40 reactions
EUR 802

Human Pancreas Acetone Powder

IRHUPCSP1GM each
EUR 422
Description: Human Pancreas Acetone Powder

Salmon pancreas disease virus

PCR-V263-PCRV26348R PCR-V263-48R
EUR 485

Salmon pancreas disease virus

PCR-V263-PCRV26396R PCR-V263-96R
EUR 685

Salmon pancreas disease virus

Oneq-V263-OneqV263100R Oneq-V263-100R
EUR 1028
Two ABEmax or mini ABEmax variants (TadA* fused with Cas9n) with deletion of R153 inside TadA and/or TadA* (named as del153/del153* and mini del153) are efficiently engineered, displaying minimized RNA off-targeting, however comparable DNA on-targeting actions. Furthermore, R153 deletion in lately reported ABE8e or ABE8s may also largely scale back their RNA off-targeting actions. Taken collectively, we develop a method to generate engineered ABEs (eABEs) with minimized RNA off-targeting actions.

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