Tpt1, an integral part of the fungal and plant tRNA splicing equipment, catalyzes switch of an inside RNA 2′-PO4 to NAD+ yielding RNA 2′-OH and ADP-ribose-1′,2′-cyclic phosphate merchandise. Right here, we report NMR buildings of the Tpt1 ortholog from the bacterium Runella slithyformis (RslTpt1), as apoenzyme and certain to NAD+. RslTpt1 consists of N- and C-terminal lobes with substantial inter-lobe dynamics within the free and NAD+-bound states. ITC measurements of RslTpt1 binding to NAD+ (KD ∼31 μM), ADP-ribose (∼96 μM) and ADP (∼123 μM) point out that substrate affinity is decided primarily by the ADP moiety; no binding of NMN or nicotinamide is noticed by ITC.
NAD+-induced chemical shift perturbations (CSPs) localize completely to the RslTpt1 C-lobe. NADP+, which accommodates an adenylate 2′-PO4 (mimicking the substrate RNA 2′-PO4), binds with decrease affinity (KD ∼1 mM) and elicits solely N-lobe CSPs. The RslTpt1·NAD+ binary advanced reveals C-lobe contacts to adenosine ribose hydroxyls (His99, Thr101), the adenine nucleobase (Asn105, Asp112, Gly113, Met117) and the nicotinamide riboside (Ser125, Gln126, Asn163, Val165), a number of of that are important for RslTpt1 exercise in vivo. Proximity of the NAD+ β-phosphate to ribose-C1″ means that it could stabilize an oxocarbenium transition-state throughout step one of the Tpt1-catalyzed response.
Reverse roles of transcription elongation components Spt4/5 and Elf1 in RNA polymerase II transcription by way of B-form versus non-B DNA buildings
Transcription elongation may be affected by quite a few forms of obstacles, resembling nucleosome, pausing sequences, DNA lesions and non-B-form DNA buildings. Spt4/5 and Elf1 are conserved transcription elongation components that promote RNA polymerase II (Pol II) bypass of nucleosome and pausing sequences. Importantly, genetic research have proven that Spt4/5 performs important roles within the transcription of expanded nucleotide repeat genes related to inherited neurological illnesses. Right here, we examine the operate of Spt4/5 and Elf1 within the transcription elongation of CTG•CAG repeat utilizing an in vitro reconstituted yeast transcription system.
We discovered that Spt4/5 helps Pol II transcribe by way of the CTG•CAG tract duplex DNA, which is in good settlement with its canonical roles in stimulating transcription elongation. In sharp distinction, surprisingly, we revealed that Spt4/5 enormously inhibits Pol II transcriptional bypass of CTG and CAG slip-out buildings. Moreover, we demonstrated that transcription elongation issue Elf1 individually and cooperatively with Spt4/5 inhibits Pol II bypass of the slip-out buildings. This research uncovers the vital purposeful interplays between template DNA buildings and the operate of transcription elongation components. This research additionally expands our understanding of the capabilities of Spt4/5 and Elf1 in transcriptional processing of trinucleotide repeat DNA.
Scoring Capabilities for Protein-RNA Advanced Construction Prediction: Advances, Functions, and Future Instructions
Protein-RNA interplay is among the many most important of organic occasions in dwelling cells, being concerned in protein synthesizing, RNA processing and transport, DNA transcription, and regulation of gene expression, and lots of different important bio-molecular actions. A radical understanding of this interplay is of paramount significance in elementary research of a wide range of important mobile processes and therapeutic utility for treatment of a broad vary of illnesses. Experimental high-resolution 3D construction willpower is the first supply of information for protein-RNA complexes.
Nevertheless, attributable to technical limitations, the prevailing methods for experimental construction willpower could not match the demand from quick rising curiosity in academia and business. This downside necessitates the choice high-throughput computational technique for protein-RNA advanced construction prediction. Just like the in silico strategies used for protein-protein and protein-DNA interactions, a dependable prediction of protein-RNA advanced construction requires a scoring operate with commensurate discriminatory energy.
Derived from decided buildings and purposed to foretell the to-be-determined buildings, the scoring operate shouldn’t be solely a predictive instrument but in addition a gauge of our data of protein-RNA interplay. On this overview, we current an outline of the standing of current scoring capabilities and the scientific precept behind their constructions in addition to their strengths and limitations. Lastly, we’ll talk about about future instructions of the scoring operate growth for protein-RNA construction prediction.
Meeting and Cryo-EM construction willpower of yeast mitochondrial RNA polymerase initiation advanced intermediates
In yeast mitochondria, transcription initiation requires meeting of mitochondrial RNA polymerase and transcription initiation issue MTF1 on the DNA promoter initiation web site. This protocol describes the purification of the part proteins and meeting of partially melted and totally melted initiation advanced states. Each states co-exist in equilibrium in the identical pattern as seen by cryoelectron microscopy (cryo-EM) and permit elucidation of MTF1’s structural roles in controlling the transition into elongation. We additional define how evaluation of the advanced by mild scattering, thermal shift assay, and ultrafiltration assay reveals reproducible outcomes. For full particulars on the use and execution of this protocol, please discuss with De Wijngaert et al. (2021).
Construction-guided engineering of adenine base editor with minimized RNA off-targeting exercise
Each adenine base editors (ABEs) and cytosine base editors (CBEs) have been lately revealed to induce transcriptome-wide RNA off-target enhancing in a information RNA-independent method. Right here we assemble a reporter system containing E.coli Hokb gene with a tRNA-like motif for strong detection of RNA enhancing actions because the optimized ABE, ABEmax, induces extremely environment friendly A-to-I (inosine) enhancing inside an E.coli tRNA-like construction. Then, we design mutations to disrupt the potential interplay between TadA and tRNAs in structure-guided ideas and discover that Arginine 153 (R153) inside TadA is important for deaminating RNAs with core tRNA-like buildings.
Pancreas Lysate |
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| 1414 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Pancreas Lysate |
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| 1469 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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cDNA - Lupus: Pancreas |
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| C1236188Lup | Biochain | 40 reactions | EUR 737 |
CK PANCREAS 25 EA* |
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| 43031-2 | Pel-Freez | 25 EA | EUR 354.66 |
Stem Cell MicroRNA Profiling Panel |
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| RA620A-1 | SBI | 10 profiles | EUR 1406.4 |
Pancreas Tumor Lysate |
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| 1334 | ProSci | 0.1 mg | EUR 304.8 |
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Description: Pancreas tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Pancreas Lupus Lysate |
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| XBL-10373 | ProSci | 0.1 mg | EUR 796.2 |
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Description: Human pancreas tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human pancreas tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the pancreas tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The pancreas tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Pancreas disease spectrum (pancreatic cancer progression) with pancreas tissue array |
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| PA2081c | TissueArray | each | EUR 594 |
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Description: Pancreas disease spectrum (pancreatic cancer progression) with pancreas tissue array, including pathology grade, TNM and clinical stage, 96 cases/192 cores, replacing PA2081b |
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Pig PANCREAS 10 EA* |
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| 59431-2 | Pel-Freez | 10 EA | EUR 382.07 |
Trypsin, Human Pancreas |
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| 7292-50 | Biovision | each | EUR 373.2 |
Trypsin, Human Pancreas |
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| TRYP15-N-50 | Alpha Diagnostics | 50 ug | EUR 270 |
Pancreas Membrane Lysate |
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| XBL-10780 | ProSci | 0.1 mg | EUR 619.8 |
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Description: Human pancreas tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
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Elastase, Porcine Pancreas |
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| P1276-250 | Biovision | each | EUR 261.6 |
MicroRNA Isolation Kit |
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| KS341025 | Biochain | 1 kit | EUR 247 |
Chymotrypsin, Human Pancreas |
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| 7538-100 | Biovision | each | EUR 438 |
Chymotrypsin, Human Pancreas |
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| CHYT15-N-100 | Alpha Diagnostics | 100 ug | EUR 343.2 |
Pancreas Cytoplasmic Lysate |
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| XBL-10779 | ProSci | 0.1 mg | EUR 273.3 |
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Description: Human pancreas tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot. |
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Total RNA - Lupus: Pancreas |
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| R1236188Lup-50 | Biochain | 50 ug | EUR 421.2 |
Rabbit PANCREAS Y 25 EA* |
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| 41231-2 | Pel-Freez | 25 EA | EUR 409.48 |
Human Pancreas Tumor lysate |
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| HTL-1334 | Alpha Diagnostics | 1 mg | EUR 927.6 |
Pancreas Tissue Slide (Normal) |
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| 11-101-10um | ProSci | 10 um | EUR 241.8 |
Pancreas Tissue Slide (Normal) |
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| 11-101-4um | ProSci | 4 um | EUR 216.6 |
Pancreas Tissue Lysate (Tumor) |
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| 1737-01 | ProSci | 0.1 mg | EUR 336.3 |
|
Description: Pancreas tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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Pancreas Tissue Lysate (Tumor) |
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| 1737-02 | ProSci | 0.1 mg | EUR 336.3 |
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Description: Pancreas tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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Pancreas Tissue Lysate (Tumor) |
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| 1738-01 | ProSci | 0.1 mg | EUR 336.3 |
|
Description: Pancreas tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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Human Pancreas Tissue Lysate |
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| 30R-AP030 | Fitzgerald | 150 ug | EUR 262.8 |
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Description: Fresh tissue lysate isolated from human pancreas |
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Insulin from bovine pancreas |
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| abx082234-25mg | Abbexa | 25 mg | EUR 260.4 |
Total Protein - Lupus: Pancreas |
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| P1236188Lup | Biochain | 1 mg | EUR 486 |
Pancreas Tissue Lysate (Normal) |
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| 1736-02 | ProSci | 0.1 mg | EUR 260.7 |
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Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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Pancreas Tissue Lysate (Normal) |
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| 1736-05 | ProSci | 0.1 mg | EUR 260.7 |
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Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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cDNA - Liver Cirrhosis: Pancreas |
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| C1236188Lcs | Biochain | 40 reactions | EUR 729 |
CK PANCREAS POWDER LYOPH 1g* |
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| 43032-0 | Pel-Freez | 1g | EUR 147.17 |
Cancer MicroRNA qPCR Array |
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| RA610A-1 | SBI | 10 profiles | EUR 1406.4 |
Pancreas Tissue Slide (Abnormal) |
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| 11-106-10um | ProSci | 10 um | EUR 241.8 |
Pancreas Tissue Slide (Abnormal) |
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| 11-106-4um | ProSci | 4 um | EUR 216.6 |
Elastase from Porcine Pancreas |
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| 30047 | Chondrex | 1 mg | EUR 119.16 |
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Description: Elastase from Porcine Pancreas |
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Alpha-Amylase, Human Pancreas |
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| P1454-100 | Biovision | each | EUR 444 |
Alpha-Amylase, Human Pancreas |
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| P1545-100 | Biovision | 100 units | EUR 417.6 |
Pancreas Membrane Tumor Lysate |
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| XBL-10795 | ProSci | 0.1 mg | EUR 1023 |
|
Description: Human pancreas tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
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CK PANCREAS POWDER LYOPH 10g* |
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| 43032-1 | Pel-Freez | 10g | EUR 400.33 |
CK PANCREAS POWDER LYOPH 100g* |
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| 43032-2 | Pel-Freez | 100g | EUR 2981.63 |
Rabbit PANCREAS MATURE 25 EA* |
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| 41331-2 | Pel-Freez | 25 EA | EUR 409.48 |
Human microRNA-210(miR-210)ELISA |
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| QY-E05678 | Qayee Biotechnology | 96T | EUR 433.2 |
Pancreas Liver Cirrhosis Lysate |
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| XBL-10372 | ProSci | 0.1 mg | EUR 796.2 |
|
Description: Human pancreas tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human pancreas tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the pancreas tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The pancreas tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Cancer of the pancreas, 102 cases (1.5mm) |
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| PAC1021 | Pantomics | 1 | EUR 354 |
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Description: Pancreatic cancer tissue array contains 96 cases of pancreatic cancers and 6 cases of normal and non-malignant pancreatic tissues with grading and TNM staging data. |
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Fetal Pancreas Cytoplasmic Lysate |
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| XBL-10791 | ProSci | 0.1 mg | EUR 273.3 |
|
Description: Human pancreas tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The fetal human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot. |
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Pancreas Cytoplasmic Tumor Lysate |
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| XBL-10794 | ProSci | 0.1 mg | EUR 273.3 |
|
Description: Human pancreas tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot. |
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Pancreatic cancer with pancreas tissue array |
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| PA2082a | TissueArray | each | EUR 546 |
|
Description: Pancreatic cancer with pancreas tissue array, including ductal adenocarcinoma(PADC),acinar cell carcinoma(PACC),normal adjacent and pancreas tissue,pathology grade, TNM and clinical stage, 96 cases/192 cores(1.0mm), replacing PA2082 |
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Pancreatic cancer with pancreas tissue array |
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| PA805c | TissueArray | each | EUR 306 |
|
Description: Pancreatic cancer with pancreas tissue array, including duct adenocarcinoma(PDAC), acinar cell carcinoma(PACC),squamous cell carcinoma,normal adjacent pancreas and normal tissuepathology grade, TNM and clinical stage, 80 cases/80 cores(1.5mm), replacing PA805b |
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Pancreatic cancer with pancreas tissue array |
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| T141c | TissueArray | each | EUR 48 |
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Description: Pancreatic cancer with pancreas tissue array, including pathology grade, TNM and clinical stage, 6 cases/24 cores, replacing T141b |
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Human Pancreas Chymotrypsin Protein |
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| abx060937-100ug | Abbexa | 100 ug | EUR 577.2 |
cDNA - Human Tumor Tissue: Pancreas |
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| C1235188-10 | Biochain | 10 reactions | EUR 274 |
Rat Pancreas Whole tissue lysate |
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| RAL-1469 | Alpha Diagnostics | 1 mg | EUR 628.8 |
Rat Pancreas PrimaCell: Normal Pancreatic Islets |
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| 2-82578 | CHI Scientific | 1 Kit | Ask for price |
Pancreas Tissue Slide (Adenocarcinoma) |
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| 11-105-10um | ProSci | 10 um | EUR 241.8 |
Pancreas Tissue Slide (Adenocarcinoma) |
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| 11-105-4um | ProSci | 4 um | EUR 216.6 |
Pig (Pancreas) alpha Amylase Enzyme |
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| abx070001-25kU | Abbexa | 25 kU | EUR 777.6 |
Mouse Pancreas Whole tissue lysate |
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| MAL-1414 | Alpha Diagnostics | 1 mg | EUR 628.8 |
Total RNA - Liver Cirrhosis: Pancreas |
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| R1236188Lcs-50 | Biochain | 50 ug | EUR 421.2 |
Mouse Pancreas PrimaCell2: Normal Pancreatic Islets |
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| 2-82081 | CHI Scientific | 1 Kit | Ask for price |
Human (Pancreas) Amylase alpha Enzyme |
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| abx070003-100Units | Abbexa | 100 Units | EUR 543.6 |
Total RNA - Diabetic Disease: Pancreas |
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| R1236188Dia-50 | Biochain | 50 ug | EUR 421.2 |
Pancreas intraepithelial neoplasia(PanIN) |
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| BIC14011b | TissueArray | each | EUR 222 |
|
Description: Pancreas intraepithelial neoplasia(PanIN), pancreatitis and cancer tissue array, including pathology grade, TNM and clinical stage, 24 cases/48 cores, replacing BIC14011a |
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MicroRNA scramble in CD511 - Virus |
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| PMIRH000VA-1 | SBI | 1x10^6 IFUs | EUR 715 |
Pancreatic adenocarcinoma with pancreas tissue array |
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| PA484a | TissueArray | each | EUR 168 |
|
Description: Pancreatic adenocarcinoma with pancreas tissue array, including pathology grade, TNM and clinical stage, 24 cases/48 cores (core size2.0mm), replacing PA484 |
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Pancreatic adenocarcinoma with pancreas tissue array |
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| PA1001c | TissueArray | each | EUR 306 |
|
Description: Pancreatic adenocarcinoma with pancreas tissue array, including pathology grade, TNM and clinical stage (reference AJCC 8th version), 50 cases/100 cores, replacing PA1001b |
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Ribonuclease A from bovine pancreas |
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| abx082524-25mg | Abbexa | 25 mg | EUR 226.8 |
Frozen Tissue Section - Lupus: Pancreas |
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| T1236188Lup | Biochain | 5 slides | EUR 515 |
Pancreatic cancer with normal pancreas tissue array |
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| PA961f | TissueArray | each | EUR 306 |
|
Description: Pancreatic cancer with normal pancreas tissue array, including pathology grade, TNM and clinical stage, 96 cases/96 cores, replacing PA961e |
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Total Protein - Liver Cirrhosis: Pancreas |
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| P1236188Lcs | Biochain | 1 mg | EUR 471 |
Paraffin Tissue Section - Lupus: Pancreas |
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| T2236188Lup | Biochain | 5 slides | EUR 243 |
Total RNA - Rat Normal Tissue: Pancreas |
|||
| R1434188-50 | Biochain | 50 ug | EUR 313 |
Rat Pancreas PrimaCell 2: Pancreatic Epithelial Cells |
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| 2-82597 | CHI Scientific | 1 Kit | Ask for price |
Pancreas Duodenum Homeobox-1 Antibody |
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| 20-abx137204 | Abbexa |
|
|
Salmon pancreas disease virus PCR kit |
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| PCR-V263-48R | Bioingentech | 50T | EUR 987.6 |
Salmon pancreas disease virus PCR kit |
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| PCR-V263-96R | Bioingentech | 100T | EUR 1335.6 |
SanPrep Column microRNA Miniprep Kit |
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| SK8811 | Bio Basic | 50prep | EUR 151.32 |
Bovine pancreas Insulin (~30 USP U/mg) |
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| INSL15-N-10 | Alpha Diagnostics | 10 mg | EUR 489.6 |
Mouse Pancreas, Peptide Aptamer, unlabeled |
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| AP-322-U | Alpha Diagnostics | 5 mg | Ask for price |
Phospholipase A2, Pancreas (pPLA2) Antibody |
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| 20-abx174050 | Abbexa |
|
|
Phospholipase A2, Pancreas (pPLA2) Antibody |
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| 20-abx178008 | Abbexa |
|
|
Phospholipase A2, Pancreas (pPLA2) Antibody |
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| 20-abx128460 | Abbexa |
|
|
Mouse Pancreas PrimaCell 2: Pancreatic Epithelial Cells |
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| 2-82102 | CHI Scientific | 1 Kit | Ask for price |
Human Pancreas PrimaCell 2: Pancreatic Epithelial Cells |
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| 2-96123 | CHI Scientific | 1 Kit | Ask for price |
Total RNA - Mouse Normal Tissue: Pancreas |
|||
| R1334188-50 | Biochain | 50 ug | EUR 369 |
Membrane Protein - Liver Cirrhosis: Pancreas |
|||
| P3236188Lcs | Biochain | 0.1 mg | EUR 410 |
Pancreas Membrane Diabetic Disease Lysate |
|||
| XBL-10785 | ProSci | 0.1 mg | EUR 752.1 |
|
Description: Human pancreas tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
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cDNA - Human Adult Normal Tissue: Pancreas |
|||
| C1234188 | Biochain | 40 reactions | EUR 385 |
Membrane Protein - Diabetic Disease: Pancreas |
|||
| P3236188Dia | Biochain | 0.1 mg | EUR 410 |
Dog Pancreastatin ELISA kit |
|||
| E08P0605-192T | BlueGene | 192 tests | EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Canine Pancreastatin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Pancreastatin ELISA kit |
|||
| E08P0605-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Canine Pancreastatin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Pancreastatin ELISA kit |
|||
| E08P0605-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Canine Pancreastatin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pancreas Dissociation System 8 (Duct), Rat |
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| 4-20378 | CHI Scientific | ea | Ask for price |
Mouse Pancreas, Peptide Aptamer, Biotinylated |
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| AP-322-B | Alpha Diagnostics | 1 mg | Ask for price |
Recombinant Phospholipase A2, Pancreas (pPLA2) |
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| 4-RPD826Hu01 | Cloud-Clone |
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Description: Recombinant Human Phospholipase A2, Pancreas expressed in: E.coli |
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cDNA - Monkey (Rhesus) Normal Tissue: Pancreas |
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| C1534188 | Biochain | 40 reactions | EUR 385 |
Total Protein - Human Tumor Tissue: Pancreas |
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| P1235188 | Biochain | 1 mg | EUR 471 |
Salmon pancreas disease virus RT PCR kit |
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| RTq-V263-100R | Bioingentech | 100T | EUR 1573.2 |
Salmon pancreas disease virus RT PCR kit |
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| RTq-V263-150R | Bioingentech | 150T | EUR 2144.4 |
Salmon pancreas disease virus RT PCR kit |
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| RTq-V263-50R | Bioingentech | 50T | EUR 1155.6 |
Pancreas Dissociation System 3 (Duct), Mouse |
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| 4-20373 | CHI Scientific | ea | Ask for price |
Deoxyribonuclease (1000 U/g), Bovine Pancreas |
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| DNS-01 | Alpha Diagnostics | 1 g | EUR 781.2 |
Total Protein - Mouse Normal Tissue: Pancreas |
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| P1334188 | Biochain | 1 mg | EUR 188 |
Frozen Tissue Section - Human Tumor: Pancreas |
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| T1235188 | Biochain | 5 slides | EUR 330 |
MicroRNA Precursor Pooled Plasmid Library |
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| PMIRHPLPA-1 | SBI | 1 aliquot plasmid DNA | EUR 60 |
Pig Phospholipase A2, Pancreas (pPLA2) Protein |
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| 20-abx654732 | Abbexa |
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rno-miRNome MicroRNA Profiling Kit [Rat] |
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| RA680B-1 | SBI | 20 complete profiles | EUR 2006.4 |
Mouse Pancreas, Peptide Aptamer, FITC labelled |
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| AP-322-F | Alpha Diagnostics | 1 mg | Ask for price |
cDNA - Human Diabetic Diseased Tissue: Pancreas |
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| C1236188Dia | Biochain | 40 reactions | EUR 729 |
Membrane Protein - Human Tumor Tissue: Pancreas |
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| P3235188 | Biochain | 0.1 mg | EUR 668 |
Paraffin Tissue Section - Human Pancreas Tumor |
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| T2235188 | Biochain | 5 slides | EUR 238 |
Rat Pancreas PrimaCell: Normal Pancreatic Islets Growth Medium |
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| 9-25078 | CHI Scientific | 5 x 100 ml | Ask for price |
cDNA - Monkey (Cynomolgus) Normal Tissue: Pancreas |
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| C1534188-Cy | Biochain | 40 reactions | EUR 385 |
Human Phospholipase A2, Pancreas (pPLA2) Protein |
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| 20-abx166479 | Abbexa |
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Monkey (Cynomolgus) cDNA Normal Tissue: Pancreas |
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| MC34-188 | Alpha Diagnostics | 10 rxn | EUR 498 |
hsa-miRNome MicroRNA Profiling Kit [Human] |
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| RA660A-1 | SBI | 20 profiles | EUR 2212.8 |
mmu-miRNome MicroRNA Profiling Kit [Mouse] |
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| RA670A-1 | SBI | 20 profiles | EUR 2046 |
Mouse Pancreas PrimaCell2: Normal Pancreatic Islets Growth Medium |
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| 9-32081 | CHI Scientific | 5 x 100 ml | Ask for price |
Rat Pancreas Tissue Preparation Buffer: Normal Pancreatic Islets |
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| 9-80285 | CHI Scientific | 1 x 100 ml | Ask for price |
Cancer of the pancreas, 24 cases (2mm), set 1 |
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| PAC481 | Pantomics | 1 | EUR 300 |
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Description: Pancreatic cancer tissue array, set 1, contains 20 cases of pancreatic cancers and 4 cases of normal and non-malignant pancreatic tissues in duplicates with grading and TNM staging data. |
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Cancer of the pancreas, 24 cases (2mm), set 2 |
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| PAC482 | Pantomics | 1 | EUR 300 |
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Description: Pancreatic cancer tissue array, set 2, contains 20 cases of pancreatic cancers and 4 cases of normal pancreatic tissues in duplicates, non-overlapping with PAC481, with grading and TNM staging data. |
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Pig Phospholipase A2, Pancreas (pPLA2) CLIA Kit |
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| 20-abx494402 | Abbexa |
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FFPE Genomic DNA - Human Tumor Tissue: Pancreas |
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| D2235188 | Biochain | 2 ug | EUR 960 |
Total RNA - Human Adult Normal Tissue: Pancreas |
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| R1234188-50 | Biochain | 50 ug | EUR 170 |
Multiple Species Tissue Slides - Pancreas Tissue |
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| 11-100-MSTA | ProSci | 1 pack | EUR 241.8 |
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Description: The Multiple Species Tissue Array (MSTA) slides were designed to study protein expression patterns in different cells and tissues from multiple species. Tissue slices from three different species are mounted on each MSTA slide which can then be treated as a single histological slide for H&E staining, immunohistochemistry, or in situ hybridization. This format allows a rapid analysis of protein expression and localization across different species. MSTA slides can also be used to quickly determine the species reactivity of a given antibody. |
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Rat Pancreas OptiTDS: Tissue Dissociation System |
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| 4-28285 | CHI Scientific | 1 Kit | Ask for price |
Pig Phospholipase A2, Pancreas (pPLA2) ELISA Kit |
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| 20-abx154993 | Abbexa |
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Pig Phospholipase A2, Pancreas (pPLA2) ELISA Kit |
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| SED826Po-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 6777.36 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Phospholipase A2, Pancreas (pPLA2) in serum, plasma, tissue homogenates and other biological fluids. |
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Pig Phospholipase A2, Pancreas (pPLA2) ELISA Kit |
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| SED826Po-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 663.31 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Phospholipase A2, Pancreas (pPLA2) in serum, plasma, tissue homogenates and other biological fluids. |
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Pig Phospholipase A2, Pancreas (pPLA2) ELISA Kit |
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| SED826Po-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 896.16 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Phospholipase A2, Pancreas (pPLA2) in serum, plasma, tissue homogenates and other biological fluids. |
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Pig Phospholipase A2, Pancreas (pPLA2) ELISA Kit |
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| SED826Po-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 3672.72 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Phospholipase A2, Pancreas (pPLA2) in serum, plasma, tissue homogenates and other biological fluids. |
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Pig Phospholipase A2, Pancreas (pPLA2) ELISA Kit |
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| 4-SED826Po | Cloud-Clone |
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Pig Phospholipase A2, Pancreas (pPLA2) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. |
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Rat Phospholipase A2(Pancreas(pPLA2)ELISA kit |
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| QY-E10541 | Qayee Biotechnology | 96T | EUR 480 |
Paraffin Tissue Section - Liver Cirrhosis: Pancreas |
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| T2236188Lcs | Biochain | 5 slides | EUR 238 |
Cholesterol esterase (35 U/mg), Porcine Pancreas |
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| CES-02 | Alpha Diagnostics | 50000 U | EUR 6554.4 |
Ribonuclease (70 U/mg material), Bovine pancreas |
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| RNU-01 | Alpha Diagnostics | 500 mg | EUR 416.4 |
Ribonuclease (90 U/mg material), Bovine pancreas |
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| RNU-02 | Alpha Diagnostics | 500 mg | EUR 562.8 |
Total RNA - Monkey (Rhesus) Normal Tissue: Pancreas |
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| R1534188-50 | Biochain | 50 ug | EUR 191 |
Trypsin (5000 NF U/mg material), Porcine pancreas |
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| TRY-01 | Alpha Diagnostics | 5 g | EUR 562.8 |
miRZip-1 anti-miR-1 microRNA construct |
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| MZIP1-PA-1 | SBI | Bacterial Streak | EUR 620 |
miRZip-7 anti-miR-7 microRNA construct |
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| MZIP7-PA-1 | SBI | Bacterial Streak | EUR 620 |
miRZip-9 anti-miR-9 microRNA construct |
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| MZIP9-PA-1 | SBI | Bacterial Streak | EUR 620 |
Human Phospholipase A2, Pancreas (pPLA2)ELISA kit |
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| 201-12-2531 | SunredBio | 96 tests | EUR 528 |
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Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
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Human Phospholipase A2, Pancreas (pPLA2) ELISA Kit |
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| 20-abx052755 | Abbexa |
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Human Phospholipase A2, Pancreas (pPLA2) ELISA Kit |
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| abx570720-96tests | Abbexa | 96 tests | EUR 943.2 |
Human Adult Pancreas (Normal) Whole tissue lysate |
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| HAL-1307 | Alpha Diagnostics | 1 mg | EUR 628.8 |
Monkey (Cyno.) Normal Pancreas Whole tissue lysate |
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| MCL-1198 | Alpha Diagnostics | 1 mg | EUR 628.8 |
Human Phospholipase A2, Pancreas(pPLA2)ELISA Kit |
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| QY-E00482 | Qayee Biotechnology | 96T | EUR 433.2 |
Mouse Phospholipase A2(Pancreas(pPLA2)ELISA kit |
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| QY-E21174 | Qayee Biotechnology | 96T | EUR 433.2 |
Human Phospholipase A2, Pancreas (pPLA2)ELISA kit |
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| YLA3198HU-48T | Shanghai YL Biotech | 48T | EUR 435 |
Human Phospholipase A2, Pancreas (pPLA2)ELISA kit |
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| YLA3198HU-96T | Shanghai YL Biotech | 96T | EUR 562.5 |
Mouse Pancreas OptiTDS2: Tissue Dissociation System |
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| 4-28195 | CHI Scientific | 1 Kit | Ask for price |
Total Protein - Human Adult Normal Tissue: Pancreas |
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| P1234188 | Biochain | 1 mg | EUR 188 |
Genomic DNA - Lupus: Pancreas, from a single donor |
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| D1236188Lup | Biochain | 50 ug | EUR 504 |
Human Pancreas Tissue Slide (normal) (5 slides/pk) |
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| HTS-11101 | Alpha Diagnostics | 1 pk | EUR 343.2 |
OncoMir pooled microRNA precursor virus library |
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| PMIRHOPLVA-1 | SBI | 1 virus aliquot | EUR 1429.2 |
miRZip-16 anti-miR-16 microRNA construct |
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| MZIP16-PA-1 | SBI | Bacterial Streak | EUR 620 |
miRZip-17 anti-miR-17 microRNA construct |
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| MZIP17-AA-1 | SBI | Miniprep DNA | EUR 620 |
miRZip-17 anti-miR-17 microRNA construct |
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| MZIP17-PA-1 | SBI | Bacterial Streak | EUR 620 |
miRZip-21 anti-miR-21 microRNA construct |
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| MZIP21-PA-1 | SBI | Bacterial Streak | EUR 620 |
miRZip-22 anti-miR-22 microRNA construct |
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| MZIP22-PA-1 | SBI | Bacterial Streak | EUR 620 |
miRZip-24 anti-miR-24 microRNA construct |
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| MZIP24-PA-1 | SBI | Bacterial Streak | EUR 620 |
miRZip-25 anti-miR-25 microRNA construct |
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| MZIP25-PA-1 | SBI | Bacterial Streak | EUR 620 |
miRZip-31 anti-miR-31 microRNA construct |
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| MZIP31-PA-1 | SBI | Bacterial Streak | EUR 620 |
miRZip-32 anti-miR-32 microRNA construct |
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| MZIP32-PA-1 | SBI | Bacterial Streak | EUR 620 |
miRZip-93 anti-miR-93 microRNA construct |
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| MZIP93-PA-1 | SBI | Bacterial Streak | EUR 620 |
Two ABEmax or mini ABEmax variants (TadA* fused with Cas9n) with deletion of R153 inside TadA and/or TadA* (named as del153/del153* and mini del153) are efficiently engineered, displaying minimized RNA off-targeting, however comparable DNA on-targeting actions. Furthermore, R153 deletion in lately reported ABE8e or ABE8s may also largely scale back their RNA off-targeting actions. Taken collectively, we develop a method to generate engineered ABEs (eABEs) with minimized RNA off-targeting actions.
