NMR solution structures of Runella slithyformis RNA 2′-phosphotransferase Tpt1 provide insights into NAD+ binding and specificity

NMR solution structures of Runella slithyformis RNA 2'-phosphotransferase Tpt1 provide insights into NAD+ binding and specificity

Tpt1, an integral part of the fungal and plant tRNA splicing equipment, catalyzes switch of an inside RNA 2′-PO4 to NAD+ yielding RNA 2′-OH and ADP-ribose-1′,2′-cyclic phosphate merchandise. Right here, we report NMR buildings of the Tpt1 ortholog from the bacterium Runella slithyformis (RslTpt1), as apoenzyme and certain to NAD+. RslTpt1 consists of N- and C-terminal lobes with substantial inter-lobe dynamics within the free and NAD+-bound states. ITC measurements of RslTpt1 binding to NAD+ (KD ∼31 μM), ADP-ribose (∼96 μM) and ADP (∼123 μM) point out that substrate affinity is decided primarily by the ADP moiety; no binding of NMN or nicotinamide is noticed by ITC.

NAD+-induced chemical shift perturbations (CSPs) localize completely to the RslTpt1 C-lobe. NADP+, which accommodates an adenylate 2′-PO4 (mimicking the substrate RNA 2′-PO4), binds with decrease affinity (KD ∼1 mM) and elicits solely N-lobe CSPs. The RslTpt1·NAD+ binary advanced reveals C-lobe contacts to adenosine ribose hydroxyls (His99, Thr101), the adenine nucleobase (Asn105, Asp112, Gly113, Met117) and the nicotinamide riboside (Ser125, Gln126, Asn163, Val165), a number of of that are important for RslTpt1 exercise in vivo. Proximity of the NAD+ β-phosphate to ribose-C1″ means that it could stabilize an oxocarbenium transition-state throughout step one of the Tpt1-catalyzed response.

Reverse roles of transcription elongation components Spt4/5 and Elf1 in RNA polymerase II transcription by way of B-form versus non-B DNA buildings

Transcription elongation may be affected by quite a few forms of obstacles, resembling nucleosome, pausing sequences, DNA lesions and non-B-form DNA buildings. Spt4/5 and Elf1 are conserved transcription elongation components that promote RNA polymerase II (Pol II) bypass of nucleosome and pausing sequences. Importantly, genetic research have proven that Spt4/5 performs important roles within the transcription of expanded nucleotide repeat genes related to inherited neurological illnesses. Right here, we examine the operate of Spt4/5 and Elf1 within the transcription elongation of CTG•CAG repeat utilizing an in vitro reconstituted yeast transcription system.
We discovered that Spt4/5 helps Pol II transcribe by way of the CTG•CAG tract duplex DNA, which is in good settlement with its canonical roles in stimulating transcription elongation. In sharp distinction, surprisingly, we revealed that Spt4/5 enormously inhibits Pol II transcriptional bypass of CTG and CAG slip-out buildings. Moreover, we demonstrated that transcription elongation issue Elf1 individually and cooperatively with Spt4/5 inhibits Pol II bypass of the slip-out buildings. This research uncovers the vital purposeful interplays between template DNA buildings and the operate of transcription elongation components. This research additionally expands our understanding of the capabilities of Spt4/5 and Elf1 in transcriptional processing of trinucleotide repeat DNA.

Scoring Capabilities for Protein-RNA Advanced Construction Prediction: Advances, Functions, and Future Instructions

Protein-RNA interplay is among the many most important of organic occasions in dwelling cells, being concerned in protein synthesizing, RNA processing and transport, DNA transcription, and regulation of gene expression, and lots of different important bio-molecular actions. A radical understanding of this interplay is of paramount significance in elementary research of a wide range of important mobile processes and therapeutic utility for treatment of a broad vary of illnesses. Experimental high-resolution 3D construction willpower is the first supply of information for protein-RNA complexes.
Nevertheless, attributable to technical limitations, the prevailing methods for experimental construction willpower could not match the demand from quick rising curiosity in academia and business. This downside necessitates the choice high-throughput computational technique for protein-RNA advanced construction prediction. Just like the in silico strategies used for protein-protein and protein-DNA interactions, a dependable prediction of protein-RNA advanced construction requires a scoring operate with commensurate discriminatory energy.
Derived from decided buildings and purposed to foretell the to-be-determined buildings, the scoring operate shouldn’t be solely a predictive instrument but in addition a gauge of our data of protein-RNA interplay. On this overview, we current an outline of the standing of current scoring capabilities and the scientific precept behind their constructions in addition to their strengths and limitations. Lastly, we’ll talk about about future instructions of the scoring operate growth for protein-RNA construction prediction.

Meeting and Cryo-EM construction willpower of yeast mitochondrial RNA polymerase initiation advanced intermediates

In yeast mitochondria, transcription initiation requires meeting of mitochondrial RNA polymerase and transcription initiation issue MTF1 on the DNA promoter initiation web site. This protocol describes the purification of the part proteins and meeting of partially melted and totally melted initiation advanced states. Each states co-exist in equilibrium in the identical pattern as seen by cryoelectron microscopy (cryo-EM) and permit elucidation of MTF1’s structural roles in controlling the transition into elongation. We additional define how evaluation of the advanced by mild scattering, thermal shift assay, and ultrafiltration assay reveals reproducible outcomes. For full particulars on the use and execution of this protocol, please discuss with De Wijngaert et al. (2021).
NMR solution structures of Runella slithyformis RNA 2'-phosphotransferase Tpt1 provide insights into NAD+ binding and specificity

Construction-guided engineering of adenine base editor with minimized RNA off-targeting exercise

Each adenine base editors (ABEs) and cytosine base editors (CBEs) have been lately revealed to induce transcriptome-wide RNA off-target enhancing in a information RNA-independent method. Right here we assemble a reporter system containing E.coli Hokb gene with a tRNA-like motif for strong detection of RNA enhancing actions because the optimized ABE, ABEmax, induces extremely environment friendly A-to-I (inosine) enhancing inside an E.coli tRNA-like construction. Then, we design mutations to disrupt the potential interplay between TadA and tRNAs in structure-guided ideas and discover that Arginine 153 (R153) inside TadA is important for deaminating RNAs with core tRNA-like buildings.

Chymotrypsin, Human Pancreas

CHYT15-N-100 100 ug
EUR 286

Trypsin, Human Pancreas

7292-50
EUR 311

Chymotrypsin, Human Pancreas

7538-100
EUR 365

Elastase, Porcine Pancreas

P1276-250
EUR 218

Pancreas Tumor Lysate

1334 0.1 mg
EUR 254
Description: Pancreas tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Pancreas Lupus Lysate

XBL-10373 0.1 mg
EUR 663.5
Description: Human pancreas tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human pancreas tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the pancreas tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The pancreas tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Pancreas Cytoplasmic Lysate

XBL-10779 0.1 mg
EUR 227.75
Description: Human pancreas tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

Pancreas Membrane Lysate

XBL-10780 0.1 mg
EUR 516.5
Description: Human pancreas tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Human Pancreas Chymotrypsin Protein

abx060937-100ug 100 ug
EUR 481

Insulin from bovine pancreas

abx082234-25mg 25 mg
EUR 217

Elastase from Porcine Pancreas

30047 1 mg
EUR 99.3
Description: Elastase from Porcine Pancreas

CK PANCREAS 25 EA*

43031-2 25 EA
EUR 295.55

Pig PANCREAS 10 EA*

59431-2 10 EA
EUR 318.39

Human Pancreas Tumor lysate

HTL-1334 1 mg
EUR 773

Alpha-Amylase, Human Pancreas

P1454-100
EUR 370

Alpha-Amylase, Human Pancreas

P1545-100 100 units
EUR 348

Human Pancreas Tissue Lysate

30R-AP030 150 ug
EUR 219
Description: Fresh tissue lysate isolated from human pancreas

cDNA from Lupus: Pancreas

C1236188Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Pancreas Tissue Slide (Normal)

11-101-10um 10 um
EUR 201.5

Pancreas Tissue Slide (Normal)

11-101-4um 4 um
EUR 180.5

Pancreas Tissue Slide (Adenocarcinoma)

11-105-10um 10 um
EUR 201.5

Pancreas Tissue Slide (Adenocarcinoma)

11-105-4um 4 um
EUR 180.5

Pancreas Tissue Slide (Abnormal)

11-106-10um 10 um
EUR 201.5

Pancreas Tissue Slide (Abnormal)

11-106-4um 4 um
EUR 180.5

Pancreas Tissue Lysate (Normal)

1736-02 0.1 mg
EUR 217.25
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Pancreas Tissue Lysate (Normal)

1736-05 0.1 mg
EUR 217.25
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Pancreas Tissue Lysate (Tumor)

1737-01 0.1 mg
EUR 280.25
Description: Pancreas tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Pancreas Tissue Lysate (Tumor)

1737-02 0.1 mg
EUR 280.25
Description: Pancreas tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Pancreas Tissue Lysate (Tumor)

1738-01 0.1 mg
EUR 280.25
Description: Pancreas tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Pancreas Liver Cirrhosis Lysate

XBL-10372 0.1 mg
EUR 663.5
Description: Human pancreas tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human pancreas tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the pancreas tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The pancreas tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Fetal Pancreas Cytoplasmic Lysate

XBL-10791 0.1 mg
EUR 227.75
Description: Human pancreas tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The fetal human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

Pancreas Cytoplasmic Tumor Lysate

XBL-10794 0.1 mg
EUR 227.75
Description: Human pancreas tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

Pancreas Membrane Tumor Lysate

XBL-10795 0.1 mg
EUR 852.5
Description: Human pancreas tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Recombinant Phospholipase A2, Pancreas (pPLA2)

4-RPD826Hu01
  • EUR 449.44
  • EUR 223.00
  • EUR 1410.40
  • EUR 536.80
  • EUR 973.60
  • EUR 364.00
  • EUR 3376.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Phospholipase A2, Pancreas expressed in: E.coli

Phospholipase A2, Pancreas (pPLA2) Antibody

20-abx128460
  • EUR 425.00
  • EUR 133.00
  • EUR 1205.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Pig (Pancreas) alpha Amylase Enzyme

abx070001-25kU 25 kU
EUR 648

Human (Pancreas) Amylase alpha Enzyme

abx070003-100Units 100 Units
EUR 453

Ribonuclease A from bovine pancreas

abx082524-25mg 25 mg
EUR 189

Rabbit PANCREAS Y 25 EA*

41231-2 25 EA
EUR 341.23

Rabbit PANCREAS MATURE 25 EA*

41331-2 25 EA
EUR 341.23

CK PANCREAS POWDER LYOPH 1g*

43032-0 1g
EUR 122.64

CK PANCREAS POWDER LYOPH 10g*

43032-1 10g
EUR 333.61

CK PANCREAS POWDER LYOPH 100g*

43032-2 100g
EUR 2484.69

Pancreas Duodenum Homeobox-1 Antibody

20-abx137204
  • EUR 453.00
  • EUR 230.00
  • EUR 328.00
  • 100 ul
  • 10 ul
  • 50 ul

Phospholipase A2, Pancreas (pPLA2) Antibody

20-abx178008
  • EUR 1414.00
  • EUR 662.00
  • 1 mg
  • 200 ug

Phospholipase A2, Pancreas (pPLA2) Antibody

20-abx174050
  • EUR 982.00
  • EUR 495.00
  • 1 mg
  • 200 ug

Rat Pancreas Whole tissue lysate

RAL-1469 1 mg
EUR 524

Mouse Pancreas Whole tissue lysate

MAL-1414 1 mg
EUR 524

Mouse Pancreas, Peptide Aptamer, Biotinylated

AP-322-B 1 mg Ask for price

Mouse Pancreas, Peptide Aptamer, unlabeled

AP-322-U 5 mg Ask for price

cDNA from Liver Cirrhosis: Pancreas

C1236188Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total Protein from Lupus: Pancreas

P1236188Lup 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Lupus: Pancreas

R1236188Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Frozen Tissue Section - Lupus: Pancreas

T1236188Lup 5 slides
EUR 474
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Lupus: Pancreas

T2236188Lup 5 slides
EUR 249
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Pancreas Membrane Diabetic Disease Lysate

XBL-10785 0.1 mg
EUR 626.75
Description: Human pancreas tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Salmon pancreas disease virus PCR kit

PCR-V263-48R 50T
EUR 823

Salmon pancreas disease virus PCR kit

PCR-V263-96R 100T
EUR 1113

Mouse Pancreas PrimaCell2: Normal Pancreatic Islets

2-82081 1 Kit Ask for price

Rat Pancreas PrimaCell: Normal Pancreatic Islets

2-82578 1 Kit Ask for price

Pig Phospholipase A2, Pancreas (pPLA2) Protein

20-abx654732
  • EUR 648.00
  • EUR 272.00
  • EUR 1998.00
  • EUR 773.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Pancreas Dissociation System 3 (Duct), Mouse

4-20373 ea Ask for price

Pancreas Dissociation System 8 (Duct), Rat

4-20378 ea Ask for price

Mouse Pancreas OptiTDS2: Tissue Dissociation System

4-28195 1 Kit Ask for price

Rat Pancreas OptiTDS: Tissue Dissociation System

4-28285 1 Kit Ask for price

Human Phospholipase A2, Pancreas (pPLA2) Protein

20-abx166479
  • EUR 634.00
  • EUR 272.00
  • EUR 1901.00
  • EUR 746.00
  • EUR 453.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Monkey (Cynomolgus) cDNA Normal Tissue: Pancreas

MC34-188 10 rxn
EUR 415

Mouse Pancreas, Peptide Aptamer, FITC labelled

AP-322-F 1 mg Ask for price

Deoxyribonuclease (1000 U/g), Bovine Pancreas

DNS-01 1 g
EUR 651

cDNA from Human Tumor Tissue: Pancreas

C1235188-10 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Diabetic Tissue: Pancreas

C1236188Dia 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total Protein from Liver Cirrhosis: Pancreas

P1236188Lcs 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein from Diabetic Disease: Pancreas

P3236188Dia 0.1 mg
EUR 408
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein from Liver Cirrhosis: Pancreas

P3236188Lcs 0.1 mg
EUR 408
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Diabetic Disease: Pancreas

R1236188Dia-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Pancreas

R1236188Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Frozen Tissue Section - Human Tumor: Pancreas

T1235188 5 slides
EUR 338
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Pancreas Tumor

T2235188 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Liver Cirrhosis: Pancreas

T2236188Lcs 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Multiple Species Tissue Slides - Pancreas Tissue

11-100-MSTA 1 pack
EUR 201.5
Description: The Multiple Species Tissue Array (MSTA) slides were designed to study protein expression patterns in different cells and tissues from multiple species. Tissue slices from three different species are mounted on each MSTA slide which can then be treated as a single histological slide for H&E staining, immunohistochemistry, or in situ hybridization. This format allows a rapid analysis of protein expression and localization across different species. MSTA slides can also be used to quickly determine the species reactivity of a given antibody.

MicroRNA Isolation Kit

KS341025 1 kit
EUR 256
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Phospholipase A2, Pancreas (pPLA2) Polyclonal Antibody (Human)

4-PAD826Hu01
  • EUR 247.00
  • EUR 2510.00
  • EUR 625.00
  • EUR 310.00
  • EUR 214.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Phospholipase A2, Pancreas (pPLA2)

Pig Phospholipase A2, Pancreas (pPLA2) ELISA Kit

SED826Po-10x96wellstestplate 10x96-wells test plate
EUR 5647.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Phospholipase A2, Pancreas (pPLA2) in serum, plasma, tissue homogenates and other biological fluids.

Pig Phospholipase A2, Pancreas (pPLA2) ELISA Kit

SED826Po-1x48wellstestplate 1x48-wells test plate
EUR 552.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Phospholipase A2, Pancreas (pPLA2) in serum, plasma, tissue homogenates and other biological fluids.

Pig Phospholipase A2, Pancreas (pPLA2) ELISA Kit

SED826Po-1x96wellstestplate 1x96-wells test plate
EUR 746.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Phospholipase A2, Pancreas (pPLA2) in serum, plasma, tissue homogenates and other biological fluids.

Pig Phospholipase A2, Pancreas (pPLA2) ELISA Kit

SED826Po-5x96wellstestplate 5x96-wells test plate
EUR 3060.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Phospholipase A2, Pancreas (pPLA2) in serum, plasma, tissue homogenates and other biological fluids.

Pig Phospholipase A2, Pancreas (pPLA2) ELISA Kit

4-SED826Po
  • EUR 5698.00
  • EUR 3011.00
  • EUR 747.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Pig Phospholipase A2, Pancreas (pPLA2) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.

Recombinant Pancreas Specific Transcription Factor 1a (PTF1a)

4-RPC738Hu01
  • EUR 530.08
  • EUR 245.00
  • EUR 1712.80
  • EUR 637.60
  • EUR 1175.20
  • EUR 418.00
  • EUR 4132.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Pancreas Specific Transcription Factor 1a expressed in: E.coli

Recombinant Pancreas Specific Transcription Factor 1a (PTF1a)

4-RPC738Hu02
  • EUR 530.08
  • EUR 245.00
  • EUR 1712.80
  • EUR 637.60
  • EUR 1175.20
  • EUR 418.00
  • EUR 4132.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Pancreas Specific Transcription Factor 1a expressed in: E.coli

Recombinant Pancreas Specific Transcription Factor 1a (PTF1a)

4-RPC738Hu03
  • EUR 413.60
  • EUR 214.00
  • EUR 1276.00
  • EUR 492.00
  • EUR 884.00
  • EUR 340.00
  • EUR 3040.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Pancreas Specific Transcription Factor 1a expressed in: E.coli

Salmon pancreas disease virus RT PCR kit

RTq-V263-100R 100T
EUR 1311

Salmon pancreas disease virus RT PCR kit

RTq-V263-150R 150T
EUR 1787
Two ABEmax or mini ABEmax variants (TadA* fused with Cas9n) with deletion of R153 inside TadA and/or TadA* (named as del153/del153* and mini del153) are efficiently engineered, displaying minimized RNA off-targeting, however comparable DNA on-targeting actions. Furthermore, R153 deletion in lately reported ABE8e or ABE8s may also largely scale back their RNA off-targeting actions. Taken collectively, we develop a method to generate engineered ABEs (eABEs) with minimized RNA off-targeting actions.

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