Tpt1, an integral part of the fungal and plant tRNA splicing equipment, catalyzes switch of an inside RNA 2′-PO4 to NAD+ yielding RNA 2′-OH and ADP-ribose-1′,2′-cyclic phosphate merchandise. Right here, we report NMR buildings of the Tpt1 ortholog from the bacterium Runella slithyformis (RslTpt1), as apoenzyme and certain to NAD+. RslTpt1 consists of N- and C-terminal lobes with substantial inter-lobe dynamics within the free and NAD+-bound states. ITC measurements of RslTpt1 binding to NAD+ (KD ∼31 μM), ADP-ribose (∼96 μM) and ADP (∼123 μM) point out that substrate affinity is decided primarily by the ADP moiety; no binding of NMN or nicotinamide is noticed by ITC.
NAD+-induced chemical shift perturbations (CSPs) localize completely to the RslTpt1 C-lobe. NADP+, which accommodates an adenylate 2′-PO4 (mimicking the substrate RNA 2′-PO4), binds with decrease affinity (KD ∼1 mM) and elicits solely N-lobe CSPs. The RslTpt1·NAD+ binary advanced reveals C-lobe contacts to adenosine ribose hydroxyls (His99, Thr101), the adenine nucleobase (Asn105, Asp112, Gly113, Met117) and the nicotinamide riboside (Ser125, Gln126, Asn163, Val165), a number of of that are important for RslTpt1 exercise in vivo. Proximity of the NAD+ β-phosphate to ribose-C1″ means that it could stabilize an oxocarbenium transition-state throughout step one of the Tpt1-catalyzed response.
Reverse roles of transcription elongation components Spt4/5 and Elf1 in RNA polymerase II transcription by way of B-form versus non-B DNA buildings
Transcription elongation may be affected by quite a few forms of obstacles, resembling nucleosome, pausing sequences, DNA lesions and non-B-form DNA buildings. Spt4/5 and Elf1 are conserved transcription elongation components that promote RNA polymerase II (Pol II) bypass of nucleosome and pausing sequences. Importantly, genetic research have proven that Spt4/5 performs important roles within the transcription of expanded nucleotide repeat genes related to inherited neurological illnesses. Right here, we examine the operate of Spt4/5 and Elf1 within the transcription elongation of CTG•CAG repeat utilizing an in vitro reconstituted yeast transcription system.
We discovered that Spt4/5 helps Pol II transcribe by way of the CTG•CAG tract duplex DNA, which is in good settlement with its canonical roles in stimulating transcription elongation. In sharp distinction, surprisingly, we revealed that Spt4/5 enormously inhibits Pol II transcriptional bypass of CTG and CAG slip-out buildings. Moreover, we demonstrated that transcription elongation issue Elf1 individually and cooperatively with Spt4/5 inhibits Pol II bypass of the slip-out buildings. This research uncovers the vital purposeful interplays between template DNA buildings and the operate of transcription elongation components. This research additionally expands our understanding of the capabilities of Spt4/5 and Elf1 in transcriptional processing of trinucleotide repeat DNA.
Scoring Capabilities for Protein-RNA Advanced Construction Prediction: Advances, Functions, and Future Instructions
Protein-RNA interplay is among the many most important of organic occasions in dwelling cells, being concerned in protein synthesizing, RNA processing and transport, DNA transcription, and regulation of gene expression, and lots of different important bio-molecular actions. A radical understanding of this interplay is of paramount significance in elementary research of a wide range of important mobile processes and therapeutic utility for treatment of a broad vary of illnesses. Experimental high-resolution 3D construction willpower is the first supply of information for protein-RNA complexes.
Nevertheless, attributable to technical limitations, the prevailing methods for experimental construction willpower could not match the demand from quick rising curiosity in academia and business. This downside necessitates the choice high-throughput computational technique for protein-RNA advanced construction prediction. Just like the in silico strategies used for protein-protein and protein-DNA interactions, a dependable prediction of protein-RNA advanced construction requires a scoring operate with commensurate discriminatory energy.
Derived from decided buildings and purposed to foretell the to-be-determined buildings, the scoring operate shouldn’t be solely a predictive instrument but in addition a gauge of our data of protein-RNA interplay. On this overview, we current an outline of the standing of current scoring capabilities and the scientific precept behind their constructions in addition to their strengths and limitations. Lastly, we’ll talk about about future instructions of the scoring operate growth for protein-RNA construction prediction.
Meeting and Cryo-EM construction willpower of yeast mitochondrial RNA polymerase initiation advanced intermediates
In yeast mitochondria, transcription initiation requires meeting of mitochondrial RNA polymerase and transcription initiation issue MTF1 on the DNA promoter initiation web site. This protocol describes the purification of the part proteins and meeting of partially melted and totally melted initiation advanced states. Each states co-exist in equilibrium in the identical pattern as seen by cryoelectron microscopy (cryo-EM) and permit elucidation of MTF1’s structural roles in controlling the transition into elongation. We additional define how evaluation of the advanced by mild scattering, thermal shift assay, and ultrafiltration assay reveals reproducible outcomes. For full particulars on the use and execution of this protocol, please discuss with De Wijngaert et al. (2021).
Construction-guided engineering of adenine base editor with minimized RNA off-targeting exercise
Each adenine base editors (ABEs) and cytosine base editors (CBEs) have been lately revealed to induce transcriptome-wide RNA off-target enhancing in a information RNA-independent method. Right here we assemble a reporter system containing E.coli Hokb gene with a tRNA-like motif for strong detection of RNA enhancing actions because the optimized ABE, ABEmax, induces extremely environment friendly A-to-I (inosine) enhancing inside an E.coli tRNA-like construction. Then, we design mutations to disrupt the potential interplay between TadA and tRNAs in structure-guided ideas and discover that Arginine 153 (R153) inside TadA is important for deaminating RNAs with core tRNA-like buildings.
Dog Pancreas Specific Transcription Factor 1a ELISA kit |
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| E08P0154-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Canine Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pancreas tumor with pancreas tissue array |
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| T143b | TissueArray | each | EUR 66 |
|
Description: Pancreas tumor with pancreas tissue array, including TNM and clinical stage, 6 cases/24 cores, replacing T143a |
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Pancreas tissue array with pancreas cancer tissue |
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| PAN241a | TissueArray | each | EUR 198 |
|
Description: Pancreas tissue array with pancreas cancer tissue, including TNM, clinical stage and pathology grade, 24 cases/24 cores, replacing PAN241 |
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Pancreas tissue array with pancreas cancer tissue |
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| PAN801 | TissueArray | each | EUR 258 |
|
Description: Pancreas tissue array with pancreas cancer tissue, including TNM, clinical stage and pathology grade, 80 cases/80 cores, replaced by PAN801a |
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Multiple tumor of pancreas and pancreas tissue array |
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| T142c | TissueArray | each | EUR 66 |
|
Description: Multiple tumor of pancreas and pancreas tissue array, including pathology grade, TNM and clinical stage, 12 cases/24 cores, replacing T142b |
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Pancreas Lysate |
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| 1469 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Pancreas Lysate |
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| 1414 | ProSci | 0.1 mg | EUR 229.2 |
|
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Pancreas, Rabbit |
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| MBS639373-25Each | MyBiosource | 25Each | EUR 380 |
Pancreas, Rabbit |
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| MBS639373-5x25Each | MyBiosource | 5x25Each | EUR 1480 |
Pancreas Lysate |
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| MBS151609-01mg | MyBiosource | 0.1mg | EUR 200 |
Pancreas Lysate |
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| MBS151609-5x01mg | MyBiosource | 5x0.1mg | EUR 880 |
Pancreas Lysate |
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| MBS151660-01mg | MyBiosource | 0.1mg | EUR 200 |
Pancreas Lysate |
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| MBS151660-5x01mg | MyBiosource | 5x0.1mg | EUR 880 |
Pancreas Lysate |
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| MBS151662-01mg | MyBiosource | 0.1mg | EUR 200 |
Pancreas Lysate |
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| MBS151662-5x01mg | MyBiosource | 5x0.1mg | EUR 880 |
Pancreatin, Porcine Pancreas |
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| MBS653745-1g | MyBiosource | 1g | EUR 175 |
Pancreatin, Porcine Pancreas |
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| MBS653745-25g | MyBiosource | 25g | EUR 325 |
Pancreatin, Porcine Pancreas |
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| MBS653745-50g | MyBiosource | 50g | EUR 505 |
Pancreatin, Porcine Pancreas |
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| MBS653745-5g | MyBiosource | 5g | EUR 225 |
Pancreatin, Porcine Pancreas |
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| MBS653745-5x50g | MyBiosource | 5x50g | EUR 2125 |
Pancreas disease spectrum (pancreatic cancer progression) with pancreas tissue array |
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| PA2081c | TissueArray | each | EUR 594 |
|
Description: Pancreas disease spectrum (pancreatic cancer progression) with pancreas tissue array, including pathology grade, TNM and clinical stage, 96 cases/192 cores, replacing PA2081b |
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microRNA mimics |
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| GC0092 | GenCefe Co Ltd | 5 nmol/2 OD | EUR 48.95 |
microRNA agomiR |
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| GC0096 | GenCefe Co Ltd | 5 nmol/2 OD | EUR 87.45 |
cDNA - Lupus: Pancreas |
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| C1236188Lup | Biochain | 40 reactions | EUR 596.05 |
Pancreas Tumor Lysate |
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| 1334 | ProSci | 0.1 mg | EUR 304.8 |
|
Description: Pancreas tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Lipase, Human Pancreas |
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| MBS173153-1000Units | MyBiosource | 1000Units | EUR 1200 |
Lipase, Human Pancreas |
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| MBS173153-100Units | MyBiosource | 100Units | EUR 350 |
Lipase, Human Pancreas |
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| MBS173153-5x1000Units | MyBiosource | 5x1000Units | EUR 5200 |
Pancreas Tumor Lysate |
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| MBS151622-01mg | MyBiosource | 0.1mg | EUR 260 |
Pancreas Tumor Lysate |
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| MBS151622-5x01mg | MyBiosource | 5x0.1mg | EUR 1140 |
Lipase, Human Pancreas |
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| MBS653675-0025mg | MyBiosource | 0.025mg | EUR 530 |
Lipase, Human Pancreas |
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| MBS653675-5x0025mg | MyBiosource | 5x0.025mg | EUR 2235 |
Pancreas Lupus Lysate |
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| XBL-10373 | ProSci | 0.1 mg | EUR 796.2 |
|
Description: Human pancreas tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human pancreas tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the pancreas tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The pancreas tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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microRNA inhibitor |
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| GC0093 | GenCefe Co Ltd | 10 nmol/2 OD | EUR 48.95 |
microRNA antagomiR |
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| GC0097 | GenCefe Co Ltd | 10 nmol/2 OD | EUR 87.45 |
Stem Cell MicroRNA Profiling Panel |
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| RA620A-1 | SBI | 10 profiles | EUR 1406.4 |
Trypsin, Human Pancreas |
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| 16-19-032000-100ug | Athens Research & Technology | 100ug | EUR 205 |
Trypsin, Human Pancreas |
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| 16-19-032000-1mg | Athens Research & Technology | 1mg | EUR 1200 |
Trypsin, Human Pancreas |
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| 7292-50 | Biovision | each | EUR 373.2 |
Trypsin, Human Pancreas |
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| MBS135619-01mg | MyBiosource | 0.1mg | EUR 495 |
Trypsin, Human Pancreas |
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| MBS135619-05mg | MyBiosource | 0.5mg | EUR 1450 |
Trypsin, Human Pancreas |
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| MBS135619-5x05mg | MyBiosource | 5x0.5mg | EUR 6330 |
Trypsin (human pancreas) |
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| GWB-2BA469 | GenWay Biotech | 0.1 mg | Ask for price |
Trypsin, Human Pancreas |
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| TRYP15-N-50 | Alpha Diagnostics | 50 ug | EUR 270 |
Trypsin, Bovine Pancreas |
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| MBS653654-1g | MyBiosource | 1g | EUR 760 |
Trypsin, Bovine Pancreas |
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| MBS653654-5x1g | MyBiosource | 5x1g | EUR 3265 |
Insulin, Porcine Pancreas |
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| MBS170511-10mg | MyBiosource | 10mg | EUR 245 |
Insulin, Porcine Pancreas |
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| MBS170511-50mg | MyBiosource | 50mg | EUR 530 |
Insulin, Porcine Pancreas |
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| MBS170511-5x50mg | MyBiosource | 5x50mg | EUR 2175 |
OORA00343-10U - PANCREAS |
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| OORA00343-10U | Aviva Systems Biology | 1Each | EUR 185 |
OORA00343-1EA - PANCREAS |
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| OORA00343-1EA | Aviva Systems Biology | 1Each | EUR 179 |
OORA00484-10U - PANCREAS |
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| OORA00484-10U | Aviva Systems Biology | 1Each | EUR 55 |
OORA00484-1EA - PANCREAS |
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| OORA00484-1EA | Aviva Systems Biology | 1Each | EUR 59 |
Pancreas Membrane Lysate |
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| XBL-10780 | ProSci | 0.1 mg | EUR 619.8 |
|
Description: Human pancreas tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
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microRNA-21-IN-2 |
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| HY-151516 | MedChemExpress | 10 mg | EUR 346.33 |
|
Description: microRNA-21-IN-2 is a potential miR-21 inhibitor with an AC50 value of 3.29 μM. microRNA-21-IN-2 can be used for the research of cancer[1]. |
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microRNA-21-IN-3 |
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| HY-153534 | MedChemExpress | 1 mg | EUR 2056.31 |
|
Description: microRNA-21-IN-3 (compound 45) can specifically bind to the precursor of oncogenic and pro-inflammatory microRNA-21 with medium nanomolar affinity, reduce cancer cell proliferation and miR-21 levels, and can be used in antitumor research[1]. |
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microRNA-21-IN-1 |
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| HY-146411 | MedChemExpress | 1 mg | EUR 2943.77 |
|
Description: microRNA-21-IN-1 (compound 7A) is an efficient microRNA inhibitor. microRNA-21-IN-1 has antiproliferative activity against Hela and HCT-116 cells with IC50s of 5.5 μM and 2.8 μM respectively, as well as promotes apoptosis of Hela cells. microRNA-21-IN-1 upregulates the expression of microRNA-21 downstream functional targets (PTEN, EGR1 and SLIT2). microRNA-21-IN-1 can be used for researching anticancer[1]. |
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microRNA-21-IN-1 |
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| T63879-10mg | TargetMol Chemicals | 10mg | Ask for price |
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Description: microRNA-21-IN-1 |
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microRNA-21-IN-1 |
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| T63879-1g | TargetMol Chemicals | 1g | Ask for price |
|
Description: microRNA-21-IN-1 |
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microRNA-21-IN-1 |
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| T63879-1mg | TargetMol Chemicals | 1mg | Ask for price |
|
Description: microRNA-21-IN-1 |
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microRNA-21-IN-1 |
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| T63879-50mg | TargetMol Chemicals | 50mg | Ask for price |
|
Description: microRNA-21-IN-1 |
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microRNA-21-IN-1 |
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| T63879-5mg | TargetMol Chemicals | 5mg | Ask for price |
|
Description: microRNA-21-IN-1 |
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microRNA-21-IN-2 |
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| T61093-10mg | TargetMol Chemicals | 10mg | Ask for price |
|
Description: microRNA-21-IN-2 |
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microRNA-21-IN-2 |
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| T61093-1g | TargetMol Chemicals | 1g | Ask for price |
|
Description: microRNA-21-IN-2 |
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microRNA-21-IN-2 |
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| T61093-1mg | TargetMol Chemicals | 1mg | Ask for price |
|
Description: microRNA-21-IN-2 |
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microRNA-21-IN-2 |
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| T61093-50mg | TargetMol Chemicals | 50mg | Ask for price |
|
Description: microRNA-21-IN-2 |
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microRNA-21-IN-2 |
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| T61093-5mg | TargetMol Chemicals | 5mg | Ask for price |
|
Description: microRNA-21-IN-2 |
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Chicken Pancreas Sections |
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| CP-313 | Zyagen | 10 slides | EUR 240 |
Elastase, Porcine Pancreas |
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| MBS634645-10mg | MyBiosource | 10mg | EUR 395 |
Elastase, Porcine Pancreas |
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| MBS634645-5x10mg | MyBiosource | 5x10mg | EUR 1640 |
Colipase, Porcine Pancreas |
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| MBS170510-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Elastase, Porcine Pancreas |
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| MBS173124-1000Units | MyBiosource | 1000Units | EUR 215 |
Elastase, Porcine Pancreas |
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| MBS173124-10KUnits | MyBiosource | 10KUnits | EUR 865 |
Elastase, Porcine Pancreas |
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| MBS173124-25KUnits | MyBiosource | 25KUnits | EUR 1585 |
Elastase, Porcine Pancreas |
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| MBS173124-5x25KUnits | MyBiosource | 5x25KUnits | EUR 6920 |
Elastase, Porcine Pancreas |
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| P1276-250 | Biovision | each | EUR 261.6 |
Human pancreas fibroblasts |
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| P0018025 | Addexbio | One Frozen vial | EUR 580 |
microRNA mimics N.C |
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| GC0094 | GenCefe Co Ltd | 5 nmol/2 OD | EUR 31.05 |
microRNA agomiR N.C |
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| GC0098 | GenCefe Co Ltd | 5 nmol/2 OD | EUR 58.05 |
Chymotrypsin, Human Pancreas |
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| 16-19-030820-100ug | Athens Research & Technology | 100ug | EUR 135 |
Chymotrypsin, Human Pancreas |
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| 16-19-030820-1mg | Athens Research & Technology | 1mg | EUR 950 |
Chymotrypsin, Human Pancreas |
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| 7538-100 | Biovision | each | EUR 438 |
Chymotrypsin, Human Pancreas |
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| CHYT15-N-100 | Alpha Diagnostics | 100 ug | EUR 343.2 |
Human Pancreas Chymotrypsin |
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| GWB-8B63CA | GenWay Biotech | 0.1 mg | Ask for price |
Kallikrein, Porcine Pancreas |
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| MBS173091-1000Units | MyBiosource | 1000Units | EUR 610 |
Kallikrein, Porcine Pancreas |
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| MBS173091-5x1000Units | MyBiosource | 5x1000Units | EUR 2545 |
Chymotrypsin, Human Pancreas |
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| MBS606650-02mg | MyBiosource | 0.2mg | EUR 705 |
Chymotrypsin, Human Pancreas |
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| MBS606650-5x02mg | MyBiosource | 5x0.2mg | EUR 3030 |
Chymotrypsin, Human Pancreas |
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| MBS612394-1mL | MyBiosource | 1mL | EUR 575 |
Chymotrypsin, Human Pancreas |
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| MBS612394-5x1mL | MyBiosource | 5x1mL | EUR 2440 |
Chymotrypsin, Human Pancreas |
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| MBS655335-01mg | MyBiosource | 0.1mg | EUR 540 |
Chymotrypsin, Human Pancreas |
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| MBS655335-5x01mg | MyBiosource | 5x0.1mg | EUR 2275 |
Chymotrypsin, Human Pancreas |
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| MBS655348-01mg | MyBiosource | 0.1mg | EUR 665 |
Chymotrypsin, Human Pancreas |
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| MBS655348-5x01mg | MyBiosource | 5x0.1mg | EUR 2850 |
Pancreas Cytoplasmic Lysate |
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| XBL-10779 | ProSci | 0.1 mg | EUR 273.3 |
|
Description: Human pancreas tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot. |
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DNase I, Bovine pancreas |
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| HY-108882 | MedChemExpress | 100 mg | EUR 32.47 |
|
Description: DNase I (EC 3.1.21.1) is an enzyme that degrade DNA, it plays a key role in the cleavage of extracellular DNA is crucial for limiting the inflammatory response and maintaining homeostasis. Exogenous deoxyribonuclease shows beneficial effects in inflammatory diseases and cancer[1]. |
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RNase A, bovine pancreas |
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| HY-129046 | MedChemExpress | 25 mg | EUR 54.11 |
|
Description: Ribonuclease A (EC 3.1.27.5) cleaves RNA 3′ to pyrimidines and actively cleaves RNA at every pyrimidine residue. Ribonuclease A catalyzes the hydrolysis of single stranded RNA in the absence of metal ions or cofactors[1][2][3]. |
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Cat Pancreas Genomic DNA |
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| FG-313 | Zyagen | 0.1mg | EUR 210 |
Pig Pancreas Genomic DNA |
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| PG-313 | Zyagen | 0.1mg | EUR 177 |
Rat Pancreas Genomic DNA |
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| RG-313 | Zyagen | 0.1mg | EUR 177 |
Pancreatin 4NF ex. Porcine Pancreas, 100U/mg |
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| 41421 | Sisco Laboratories | 100 Gms | EUR 21.75 |
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Description: Part B |
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microRNA inhibitor N.C |
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| GC0095 | GenCefe Co Ltd | 10 nmol/2 OD | EUR 31.05 |
microRNA antagomiR N.C |
|||
| GC0099 | GenCefe Co Ltd | 10 nmol/2 OD | EUR 58.05 |
Dog Pancreastatin ELISA kit |
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| E08P0605-192T | BlueGene | 192 tests | EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Canine Pancreastatin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Pancreastatin ELISA kit |
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| E08P0605-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Canine Pancreastatin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Pancreastatin ELISA kit |
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| E08P0605-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Canine Pancreastatin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Pancreas Tissue Lysate |
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| IRTPCSTL100UG | Innovative research | each | EUR 245 |
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Description: Rat Pancreas Tissue Lysate |
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Human Pancreas Genomic DNA |
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| HG-313 | Zyagen | 0.05mg | EUR 210 |
Cat Pancreas Total Protein |
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| FT-313 | Zyagen | 1mg | EUR 176 |
a-Amylase, Human Pancreas |
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| MBS173014-1000Units | MyBiosource | 1000Units | EUR 1200 |
a-Amylase, Human Pancreas |
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| MBS173014-5x1000Units | MyBiosource | 5x1000Units | EUR 5200 |
Rat Pancreas Tissue Lysate |
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| MBS8421517-01mg | MyBiosource | 0.1mg | EUR 385 |
Rat Pancreas Tissue Lysate |
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| MBS8421517-5x01mg | MyBiosource | 5x0.1mg | EUR 1540 |
Pig Pancreas Total Protein |
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| PT-313 | Zyagen | 1mg | EUR 153 |
Mouse Pancreas Genomic DNA |
|||
| MG-313 | Zyagen | 0.1mg | EUR 177 |
Rat Pancreas Total Protein |
|||
| RT-313 | Zyagen | 1mg | EUR 153 |
Sheep Pancreas Genomic DNA |
|||
| SG-313 | Zyagen | 0.1mg | EUR 177 |
Bovine Pancreas Genomic DNA |
|||
| BG-313 | Zyagen | 0.1mg | EUR 177 |
Equine Pancreas Genomic DNA |
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| GE-313 | Zyagen | 0.1mg | EUR 210 |
Human Pancreas Tumor lysate |
|||
| HTL-1334 | Alpha Diagnostics | 1 mg | EUR 927.6 |
Trypsin from bovin pancreas |
|||
| MBS5788862-INQUIRE | MyBiosource | INQUIRE | Ask for price |
OORA00540-1U - Rat PANCREAS |
|||
| OORA00540-1U | Aviva Systems Biology | 1Each | EUR 60 |
Rabbit Pancreas Genomic DNA |
|||
| TG-313 | Zyagen | 0.1mg | EUR 177 |
Trypsin from bovin pancreas |
|||
| TN6872-10mg | TargetMol Chemicals | 10mg | Ask for price |
|
Description: Trypsin from bovin pancreas |
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Trypsin from bovin pancreas |
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| TN6872-1g | TargetMol Chemicals | 1g | Ask for price |
|
Description: Trypsin from bovin pancreas |
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Trypsin from bovin pancreas |
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| TN6872-1mg | TargetMol Chemicals | 1mg | Ask for price |
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Description: Trypsin from bovin pancreas |
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Trypsin from bovin pancreas |
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| TN6872-50mg | TargetMol Chemicals | 50mg | Ask for price |
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Description: Trypsin from bovin pancreas |
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Trypsin from bovin pancreas |
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| TN6872-5mg | TargetMol Chemicals | 5mg | Ask for price |
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Description: Trypsin from bovin pancreas |
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Human Pancreas Tissue Lysate |
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| 30R-AP030 | Fitzgerald | 150 ug | EUR 210 |
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Description: Fresh tissue lysate isolated from human pancreas |
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Lipase from Porcine Pancreas |
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| 20552-02 | NACALAI TESQUE | 25G | EUR 26.25 |
Pancreas Tissue Lysate (Tumor) |
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| 1737-01 | ProSci | 0.1 mg | EUR 336.3 |
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Description: Pancreas tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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Pancreas Tissue Lysate (Tumor) |
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| 1737-02 | ProSci | 0.1 mg | EUR 336.3 |
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Description: Pancreas tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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Pancreas Tissue Lysate (Tumor) |
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| 1738-01 | ProSci | 0.1 mg | EUR 336.3 |
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Description: Pancreas tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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Insulin from bovine pancreas |
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| abx082234-100l | Abbexa | 100 µl | EUR 212.5 |
Insulin from bovine pancreas |
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| abx082234-1ml | Abbexa | 1 ml | Ask for price |
Insulin from bovine pancreas |
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| abx082234-200l | Abbexa | 200 µl | Ask for price |
Insulin from bovine pancreas |
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| abx082234-25mg | Abbexa | 25 mg | EUR 260.4 |
Pancreas Tissue Slide (Normal) |
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| 11-101-10um | ProSci | 10 um | EUR 241.8 |
Pancreas Tissue Slide (Normal) |
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| 11-101-4um | ProSci | 4 um | EUR 216.6 |
Hamster Pancreas Genomic DNA |
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| GA-313 | Zyagen | 0.1mg | EUR 177 |
Chicken Pancreas Genomic DNA |
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| GC-313 | Zyagen | 0.1mg | EUR 177 |
Human Pancreas Total Protein |
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| HT-313 | Zyagen | 1mg | EUR 176 |
Human Pancreas Amylase alpha |
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| GWB-759CBC | GenWay Biotech | 100 Units | Ask for price |
Mouse Pancreas Tissue Lysate |
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| IMSPCSTL100UG | Innovative research | each | EUR 245 |
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Description: Mouse Pancreas Tissue Lysate |
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Cat Pancreas Frozen Sections |
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| FF-313 | Zyagen | 10 slides | EUR 261 |
Human Pancreas Tissue Lysate |
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| MBS537637-015mg | MyBiosource | 0.15mg | EUR 380 |
Human Pancreas Tissue Lysate |
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| MBS537637-5x015mg | MyBiosource | 5x0.15mg | EUR 1550 |
Pancreas Tissue Slide (Normal) |
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| MBS154294-10um | MyBiosource | 10um | EUR 210 |
Pancreas Tissue Slide (Normal) |
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| MBS154294-4um | MyBiosource | 4um | EUR 195 |
Pancreas Tissue Slide (Normal) |
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| MBS154294-5x10um | MyBiosource | 5x10um | EUR 920 |
Amylase, alpha, Human Pancreas |
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| MBS653881-100Units | MyBiosource | 100Units | EUR 490 |
Amylase, alpha, Human Pancreas |
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| MBS653881-5x100Units | MyBiosource | 5x100Units | EUR 2050 |
Mouse Pancreas Tissue Lysate |
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| MBS8420796-01mg | MyBiosource | 0.1mg | EUR 385 |
Mouse Pancreas Tissue Lysate |
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| MBS8420796-5x01mg | MyBiosource | 5x0.1mg | EUR 1540 |
OORA00540-1EA - Rat PANCREAS |
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| OORA00540-1EA | Aviva Systems Biology | 1Each | EUR 59 |
Pig Pancreas Frozen Sections |
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| PF-313 | Zyagen | 10 slides | EUR 261 |
Total-RNA---Lupus:-Pancreas |
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| R1236188Lup-50 | Biochain | 50 ug | EUR 338.1 |
Sheep Pancreas Total Protein |
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| ST-313 | Zyagen | 1mg | EUR 153 |
Rat Pancreas Frozen Sections |
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| RF-313 | Zyagen | 10 slides | EUR 228 |
43031-2 CK PANCREAS 25 EA* |
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| 43031-2 | Pel-Freez | 25 EA | EUR 417 |
microRNA Purification Kit |
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| 21300 | Norgen Biotek Corp | 25 Preps | EUR 149.4 |
Trypsin from Porcine Pancreas |
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| 35547-51 | NACALAI TESQUE | 1G | EUR 19.25 |
Trypsin from Porcine Pancreas |
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| 35547-64 | NACALAI TESQUE | 10G | EUR 92.4 |
Pancreas Tissue Lysate (Normal) |
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| 1736-02 | ProSci | 0.1 mg | EUR 260.7 |
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Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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Pancreas Tissue Lysate (Normal) |
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| 1736-05 | ProSci | 0.1 mg | EUR 260.7 |
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Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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Anti-Trypsin, Human Pancreas |
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| 01-19-032000-1ml | Athens Research & Technology | 1ml | EUR 125 |
Two ABEmax or mini ABEmax variants (TadA* fused with Cas9n) with deletion of R153 inside TadA and/or TadA* (named as del153/del153* and mini del153) are efficiently engineered, displaying minimized RNA off-targeting, however comparable DNA on-targeting actions. Furthermore, R153 deletion in lately reported ABE8e or ABE8s may also largely scale back their RNA off-targeting actions. Taken collectively, we develop a method to generate engineered ABEs (eABEs) with minimized RNA off-targeting actions.
